{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T01:39:35Z","timestamp":1771033175240,"version":"3.50.1"},"reference-count":33,"publisher":"Institute of Physiology of the Czech Academy of Sciences","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Physiol Res"],"abstract":"<jats:p>Sex-related differences were observed not only in human but also in experimental hypertension. The aim of our study was to compare blood pressure (BP) of aged male and female heterozygous transgenic rats (TGR) harboring Ren-2 mouse gene, with their normotensive Hannover Sprague-Dawley (HanSD) controls. At the age of 9 months, systolic (SBP) and diastolic blood pressure (DBP) were measured by a direct puncture of carotid artery in rats awaking from isoflurane anesthesia. Thiobarbituric acid-reactive species (TBARS) formation was monitored as indicator of lipid peroxidation damage in heart, kidney and liver, whereas intracellular content of reduced glutathione was determined in the same organs as the main intracellular antioxidant. Furthermore, plasma triglycerides and total cholesterol as well as high-density lipoprotein (HDL) and low-density lipoprotein (LDL) fractions of cholesterol were measured. As compared to HanSD rats, we found significantly elevated BP only in male TGR (MAP: 123\u00b11 vs. 171\u00b15, SBP: 150\u00b12 vs. 208\u00b17, and DBP: 99\u00b13 vs. 140\u00b14 mm Hg), but not between TGR and HanSD females, which were both normotensive. We also did not find any significant differences in TBARS and reduced glutathione in the three above mentioned organs as well as in plasma cholesterol or its HDL and LDL fractions between transgene-negative HanSD and TGR animals of either sex. However, we found significant sex differences in TBARS, glutathione and plasma lipids in both rat strains. Our results confirmed that aged TGR exhibit a marked sexual BP dimorphism, which does not seem to be dependent on oxidative stress or abnormal cholesterol metabolism.<\/jats:p>","DOI":"10.33549\/physiolres.934369","type":"journal-article","created":{"date-parts":[[2020,5,8]],"date-time":"2020-05-08T19:05:02Z","timestamp":1588964702000},"page":"245-252","source":"Crossref","is-referenced-by-count":8,"title":["Sex differences in blood pressure of aged Ren-2 transgenic rats"],"prefix":"10.33549","author":[{"given":"H.","family":"Rauchov\u00e1","sequence":"first","affiliation":[{"name":"Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic. 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Physiol Res 64: 303-312, 2015a. https:\/\/doi.org\/10.33549\/physiolres.933054","DOI":"10.33549\/physiolres.933054"},{"key":"ref29","doi-asserted-by":"publisher","unstructured":"VOKURKOV\u00c1 M, RAUCHOV\u00c1 H, \u0158EZ\u00c1\u010cOV\u00c1 L, VAN\u011a\u010cKOV\u00c1 I, ZICHA J: NADPH oxidase activity and reactive oxygen species production in brain and kidney of adult male hypertensive Ren-2 transgenic rats. Physiol Res 64: 849-856, 2015b. https:\/\/doi.org\/10.33549\/physiolres.933254","DOI":"10.33549\/physiolres.933254"},{"key":"ref30","doi-asserted-by":"publisher","unstructured":"WEI Y, CLARK SE, MORRIS EM, THYFAULT JP, UPTERGROVE GM, WHALEY-CONNELL AT, FERRARIO CM, SOWERS JR, IBDAH JA: Angiotensin II-induced non-alcoholic fatty liver disease is mediated by oxidative stress in transgenic TG(mRen2)27(Ren2) rats. J Hepatol 49: 417-428, 2008. https:\/\/doi.org\/10.1016\/j.jhep.2008.03.018","DOI":"10.1016\/j.jhep.2008.03.018"},{"key":"ref31","doi-asserted-by":"publisher","unstructured":"WEI Y, CLARK SE, THYFAULT JP, UPTERGROVE GM, LI W, WHALEY-CONNELL AT, FERRARIO CM, SOWERS JR, IBDAH JA: Oxidative stress-mediated mitochondrial dysfunction contributes to angiotensin II-induced nonalcoholic fatty liver disease in transgenic Ren2 rats. Am J Pathol 174: 1329-1337, 2009. https:\/\/doi.org\/10.2353\/ajpath.2009.080697","DOI":"10.2353\/ajpath.2009.080697"},{"key":"ref32","doi-asserted-by":"publisher","unstructured":"ZICHA J, DOBE\u0160OV\u00c1 Z, VOKURKOV\u00c1 M, RAUCHOV\u00c1 H, HOJN\u00c1 S, KADLECOV\u00c1 M, BEHULIAK M, VAN\u011a\u010cKOV\u00c1 I, KUNE\u0160 J: Age-dependent salt hypertension in Dahl rats: fifty years of research. 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