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Short tandem repeat profile, the doubling time, cell diameter, glutathione concentration, and intracellular dehydrogenase activity were measured in HK-2 cells at each tested passage. The results showed that HK-2 cells exhibit stable morphology, cell size, and cell renewal during passaging. Mean doubling time was determined to be 54 h. On the other hand, we observed a significant effect of passaging on the susceptibility of HK-2 cells to toxic compounds. The largest difference in results was found in both cadmium and cisplatin treated cells across passages. We conclude that the outcomes of scientific studies on HK-2 cells can be affected by the number of passages even after medium-term cultivation and passaging for 13 weeks.<\/jats:p>","DOI":"10.33549\/physiolres.934491","type":"journal-article","created":{"date-parts":[[2020,8,27]],"date-time":"2020-08-27T13:39:42Z","timestamp":1598535582000},"page":"731-738","source":"Crossref","is-referenced-by-count":11,"title":["The effect of repeated passaging on the susceptibility of human proximal tubular HK-2 cells to toxic compounds"],"prefix":"10.33549","author":[{"given":"J","family":"Handl","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"\u010capek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P","family":"Majtnerov\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"B\u00e1\u010dov\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Rou\u0161ar","sequence":"additional","affiliation":[{"name":"Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice, Pardubice, Czech Republic. 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