{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T13:54:08Z","timestamp":1770990848767,"version":"3.50.1"},"reference-count":23,"publisher":"Institute of Physiology of the Czech Academy of Sciences","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Physiol Res"],"published-print":{"date-parts":[[2021,12,30]]},"abstract":"<jats:p>In functional arterial studies using wire myography, the\ndetermination of a vessel\u2019s standardized normalization factor\n(factor k) is an essential step to ensure optimal contraction and\nrelaxation by the arteries when stimulated with their respective\nvasoactive agents and to obtain reproducible results. The optimal\nfactor k for several arteries have been determined, however, the\noptimal initial tension and factor k for the arteries involved in\nerection remains unknown. Hence, in the present study we set\nout to determine the optimal factor k for the internal iliac artery,\nproximal and distal internal pudendal artery (IPA), and dorsal\npenile artery. After isolating, harvesting, and mounting the\narteries from male Sprague-Dawley rats on a multi wire\nmyograph, we tested arterial responsivity to high K+-stimulation\nwhen the factor k was set at 0.7, 0.8, 0.85, 0.9, 0.95, 1.0, 1.1,\nand 1.2 to determine the factor k setting that results in the\ngreatest K+-induced active force production for each vessel type.\nThe data showed the optimal factor k is 0.90-0.95 for the dorsal\npenile, distal internal pudendal and internal iliac arteries while it\nis 0.85-0.90 for proximal internal pudendal artery. These optimal\nvalues corresponded to initial passive tension settings of\n1.10\u00b10.16 - 1.46\u00b10.23, 1.28\u00b10.20 - 1.69\u00b10.34, 1.03\u00b10.27 -\n1.33\u00b10.31, and 1.33\u00b10.31 - 1.77\u00b10.43 mN\/mm for the dorsal\npenile, distal IP, proximal IP, and internal iliac arteries,\nrespectively.<\/jats:p>","DOI":"10.33549\/physiolres.934714","type":"journal-article","created":{"date-parts":[[2022,3,2]],"date-time":"2022-03-02T20:16:24Z","timestamp":1646252184000},"page":"931-937","source":"Crossref","is-referenced-by-count":8,"title":["Optimal Wire Myography Normalization for the Rat Dorsal Penile, Internal Pudendal and Internal Iliac Arteries"],"prefix":"10.33549","author":[{"given":"TA","family":"Azeez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MR","family":"Andrade","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JD","family":"La Favor","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"19169","published-online":{"date-parts":[[2021,12,30]]},"reference":[{"key":"ref0","doi-asserted-by":"publisher","unstructured":"ALVES-LOPES R, NEVES KB, SILVA MAB, OLIVON VC, RUGINSK SG, ANTUNES-RODRIGUES J, RAMALHO LNZ, TOSTES RC, CARNEIRO FS: Functional and structural changes in internal pudendal arteries underlie erectile dysfunction induced by androgen deprivation. Asian J Androl 18: 526-532, 2016. https:\/\/doi.org\/10.4103\/1008-682X.173935","DOI":"10.4103\/1008-682x.173935"},{"key":"ref1","doi-asserted-by":"publisher","unstructured":"BURNETT AL, LOWENSTEIN CJ, BREDT DS, CHANG TSK, SNYDER SH: Nitric oxide: A physiologic mediator of penile erection. Science 257: 401-403, 1992. https:\/\/doi.org\/10.1126\/science.1378650","DOI":"10.1126\/science.1378650"},{"key":"ref2","doi-asserted-by":"publisher","unstructured":"CONTRERAS C, S\u00c1NCHEZ A, MART\u00cdNEZ P, CLIMENT B, BENEDITO S, GARC\u00cdA-SACRIST\u00c1N A, HERN\u00c1NDEZ M, PRIETO D: Impaired endothelin calcium signaling coupled to endothelin type b receptors in penile arteries from insulin-resistant obese Zucker rats. J Sex Med 10: 2141-2153, 2013. https:\/\/doi.org\/10.1111\/jsm.12234","DOI":"10.1111\/jsm.12234"},{"key":"ref3","doi-asserted-by":"crossref","unstructured":"GIULIANO F: Neurophysiology of Erection and Ejaculation. J Sex Med 8: 310-315, 2011. https:\/\/doi.org\/10.1111\/j.1743-6109.2011.02450.x","DOI":"10.1111\/j.1743-6109.2011.02450.x"},{"key":"ref4","doi-asserted-by":"publisher","unstructured":"GRANN M, COMERMA-STEFFENSEN S, ARCANJO DDR, SIMONSEN U: Mechanisms Involved in Thromboxane A2 -induced Vasoconstriction of Rat Intracavernous Small Penile Arteries. Basic Clinical Pharmacol Toxicol 119: 86-95, 2016. https:\/\/doi.org\/10.1111\/bcpt.12544","DOI":"10.1111\/bcpt.12544"},{"key":"ref5","doi-asserted-by":"publisher","unstructured":"HANNAN JL, BLASER MC, PANG JJ, ADAMS SM, PANG SC, ADAMS MA: Impact of hypertension, aging, and antihypertensive treatment on the morphology of the pudendal artery. J Sex Med 8: 1027-1038, 2011. https:\/\/doi.org\/10.1111\/j.1743-6109.2010.02191.x","DOI":"10.1111\/j.1743-6109.2010.02191.x"},{"key":"ref6","doi-asserted-by":"publisher","unstructured":"HANNAN JL, BLASER MC, OLDFIELD L, PANG JJ, ADAMS SM, PANG SC, ADAMS MA: Morphological and functional evidence for the contribution of the pudendal artery in aging-induced erectile dysfunction. J Sex Med 7: 3373-3384, 2010. https:\/\/doi.org\/10.1111\/j.1743-6109.2010.01920.x","DOI":"10.1111\/j.1743-6109.2010.01920.x"},{"key":"ref7","doi-asserted-by":"publisher","unstructured":"KASSAN M, LASKER GF, SIKKA SC, MANDAVA SH, GOKCE A, MATROUGUI K, HELLSTROM WJG, KADOWITZ PJ, SEREFOGLU EC: Chronic escitalopram treatment induces erectile dysfunction by decreasing nitric oxide bioavailability mediated by increased nicotinamide adenine dinucleotide phosphate oxidase activity and reactive oxygen species production. Urology 82: 1188.e1-1188.e7, 2013. https:\/\/doi.org\/10.1016\/j.urology.2013.07.037","DOI":"10.1016\/j.urology.2013.07.037"},{"key":"ref8","doi-asserted-by":"publisher","unstructured":"MAIO MT, MCCABE KM, PRUSS CM, PANG JJ, LAVERTY K, HOLDEN RM, ADAMS MA: Calcification of the internal pudendal artery and development of erectile dysfunction in adenine-induced chronic kidney disease: A sentinel of systemic vascular changes. J Sex Med 11: 2449-2465, 2014. https:\/\/doi.org\/10.1111\/jsm.12648","DOI":"10.1111\/jsm.12648"},{"key":"ref9","doi-asserted-by":"publisher","unstructured":"MULVANY MJ, HALPERN W: Contractile properties of small arterial resistance vessels in spontaneously hypertensive and normotensive rats. Circ Res 41: 19-26, 1977. https:\/\/doi.org\/10.1161\/01.RES.41.1.19","DOI":"10.1161\/01.res.41.1.19"},{"key":"ref10","doi-asserted-by":"publisher","unstructured":"MULVANY MJ, WARSHAW DM: The active tension-length curve of vascular smooth muscle related to its cellular components. J Gen Physiol 74: 85-104, 1979. https:\/\/doi.org\/10.1085\/jgp.74.1.85","DOI":"10.1085\/jgp.74.1.85"},{"key":"ref11","doi-asserted-by":"publisher","unstructured":"ODOM MR, PAK ES, BROWN DA, HANNAN JL: Enhanced electrical field stimulated nitrergic and purinergic vasoreactivity in distal vs proximal internal pudendal arteries. J Sex Med 14: 1285-1296, 2017. https:\/\/doi.org\/10.1016\/j.jsxm.2017.09.013","DOI":"10.1016\/j.jsxm.2017.09.013"},{"key":"ref12","doi-asserted-by":"publisher","unstructured":"PING NN, CAO L, XIAO X, LI S, CAO YX: The determination of optimal initial tension in rat coronary artery using wire myography. Physiol Res 63: 143-146, 2014. https:\/\/doi.org\/10.33549\/physiolres.932631","DOI":"10.33549\/physiolres.932631"},{"key":"ref13","doi-asserted-by":"publisher","unstructured":"RODRIGUES FL, LOPES RAM, FAIS RS, DE OLIVEIRA L, PRADO CM, TOSTES RC, CARNEIRO FS: Erectile dysfunction in heart failure rats is associated with increased neurogenic contractions in cavernous tissue and internal pudendal artery. Life Sci, 145: 9-18, 2016. https:\/\/doi.org\/10.1016\/j.lfs.2015.12.005","DOI":"10.1016\/j.lfs.2015.12.005"},{"key":"ref14","doi-asserted-by":"publisher","unstructured":"S\u00c1NCHEZ A, VILLALBA N, MART\u00cdNEZ AC, GARC\u00cdA-SACRIST\u00c1N A, HERN\u00c1NDEZ M, PRIETO D: Mechanisms of the relaxant effect of vardenafil in rat penile arteries. Eur J Pharmacol 586: 283-287, 2008. https:\/\/doi.org\/10.1016\/j.ejphar.2008.03.002","DOI":"10.1016\/j.ejphar.2008.03.002"},{"key":"ref15","doi-asserted-by":"publisher","unstructured":"S\u00c1NCHEZ A, CONTRERAS C, MART\u00cdNEZ MP, CLIMENT B, BENEDITO S, GARC\u00cdA-SACRIST\u00c1N A, HERN\u00c1NDEZ M, PRIETO D: Role of neural no synthase (NNOS) uncoupling in the dysfunctional nitrergic vasorelaxation of penile arteries from insulin-resistant obese zucker rats. PLoS ONE 7: e36027, 2012. https:\/\/doi.org\/10.1371\/journal.pone.0036027","DOI":"10.1371\/journal.pone.0036027"},{"key":"ref16","doi-asserted-by":"publisher","unstructured":"SILVA DF, WENCESLAU CF, MCCARTHY CG, SZASZ T, OGBI S, WEBB RC: TRPM8 channel activation triggers relaxation of pudendal artery with increased sensitivity in the hypertensive rats. Pharmacol Res 147: 104329, 2019. https:\/\/doi.org\/10.1016\/j.phrs.2019.104329","DOI":"10.1016\/j.phrs.2019.104329"},{"key":"ref17","doi-asserted-by":"publisher","unstructured":"SLEZ\u00c1K P, WACZUL\u00cdKOV\u00c1 I, BALI\u0160 P, P\u00daZSEROV\u00c1 A: Accurate normalization factor for wire myography of rat femoral artery. Physiol Res 59: 1033-1036, 2010. https:\/\/doi.org\/10.33549\/physiolres.932043","DOI":"10.33549\/physiolres.932043"},{"key":"ref18","doi-asserted-by":"publisher","unstructured":"SUN J, YANG GM, TAO T, WEI LS, PAN Y, ZHU MS: Isometric contractility measurement of the mouse mesenteric artery using wire myography. J Vis Exp 138: 58064, 2018. https:\/\/doi.org\/10.3791\/58064","DOI":"10.3791\/58064"},{"key":"ref19","doi-asserted-by":"publisher","unstructured":"VILLALBA N, STANKEVICIUS E, GARCIA-SACRIST\u00c1N A, SIMONSEN U, PRIETO D: Contribution of both Ca2+ entry and Ca2+ sensitization to the \u03b11-adrenergic vasoconstriction of rat penile small arteries. Am J Physiol Heart Circ Physiol 292: H1157-1169, 2007. https:\/\/doi.org\/10.1152\/ajpheart.01034.2006","DOI":"10.1152\/ajpheart.01034.2006"},{"key":"ref20","doi-asserted-by":"publisher","unstructured":"XIAO X, PING NN, LI S, CAO L, CAO YX: An optimal initial tension for rat basilar artery in wire myography. Microvasc Res 97: 156-158, 2015. https:\/\/doi.org\/10.1016\/j.mvr.2014.11.001","DOI":"10.1016\/j.mvr.2014.11.001"},{"key":"ref21","doi-asserted-by":"publisher","unstructured":"YAFI FA, JENKINS L, ALBERSEN M, CORONA G, ISIDORI AM, GOLDFARB S, MAGGI M, NELSON CJ, PARISH S, SALONIA A, TAN R, MULHALL JP, HELLSTROM WJG: Erectile dysfunction. Nat Rev Dis Prim 2: 16003, 2016. https:\/\/doi.org\/10.1038\/nrdp.2016.3","DOI":"10.1038\/nrdp.2016.3"},{"key":"ref22","doi-asserted-by":"publisher","unstructured":"ZHANG RZ, GASHEV AA, ZAWIEJA DC, DAVIS MJ: Length-tension relationships of small arteries, veins, and lymphatics from the rat mesenteric microcirculation. Am J Physiol Heart Circ Physiol 292: H1943-1952, 2007. https:\/\/doi.org\/10.1152\/ajpheart.01000.2005","DOI":"10.1152\/ajpheart.01000.2005"}],"container-title":["Physiological Research"],"original-title":[],"language":"en","deposited":{"date-parts":[[2022,3,2]],"date-time":"2022-03-02T20:16:32Z","timestamp":1646252192000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.biomed.cas.cz\/physiolres\/pdf\/2021\/70_931.pdf"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,12,30]]},"references-count":23,"URL":"https:\/\/doi.org\/10.33549\/physiolres.934714","relation":{},"ISSN":["1802-9973","0862-8408"],"issn-type":[{"value":"1802-9973","type":"electronic"},{"value":"0862-8408","type":"print"}],"subject":[],"published":{"date-parts":[[2021,12,30]]}}}