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Animals were assigned into control rats, non-treated diabetic rats with NAFLD, and diabetic rats with NAFLD that received fasudil treatment (10 mg\/kg per day) for 6 weeks. The anthropometric measures and biochemical analyses were performed to assess metabolic and liver function changes. The inflammatory and oxidative stress markers and the histopathology of rat liver tissues were also investigated. Groups with T2DM showed increased body weight, serum glucose, and insulin resistance. They exhibited disturbed lipid profile, enhancement of inflammatory cytokines, and deterioration of liver function. Fasudil administration reduced body weight, insulin resistance, and raised liver enzymes. It improved the disturbed lipid profile and attenuated liver inflammation. Moreover, it slowed down the progression of high fat diet (HFD)-induced liver injury and reduced the caspase-3 expression. The present study demonstrated beneficial amelioration effect of fasudil on NAFLD in T2DM. The mechanisms underlying these impacts are improving dyslipidemia, attenuating oxidative stress, downregulated inflammation, improving mitochondrial architecture, and inhibiting apoptosis.<\/jats:p>","DOI":"10.33549\/physiolres.934869","type":"journal-article","created":{"date-parts":[[2023,1,4]],"date-time":"2023-01-04T09:05:55Z","timestamp":1672823155000},"page":"615-630","source":"Crossref","is-referenced-by-count":9,"title":["Rho-Kinase Inhibition Ameliorates Non-Alcoholic Fatty Liver Disease in Type 2 Diabetic Rats"],"prefix":"10.33549","author":[{"given":"H","family":"ELKATTAWY","sequence":"first","affiliation":[{"name":"Department of Basic Medical Sciences, College of Medicine, Almaarefa University, Riyadh, Kingdom of Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D","family":"MAHMOUD ABDELMONEM ELSHERBINI","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Sakaka, Kingdom of Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"ALI EBRAHIM","sequence":"additional","affiliation":[{"name":"Department of Basic Medical Sciences, College of Medicine, Princess Nourah bint Abdulrahman University, Riyadh, Kingdom of Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D","family":"ABDULLAH","sequence":"additional","affiliation":[{"name":"Clinical Pharmacology Department, College of Medicine, Zagazig University, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"AL-ZAHABY","sequence":"additional","affiliation":[{"name":"Zoology Department, College of Sciences, Zagazig University, Egypt,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y","family":"NOSERY","sequence":"additional","affiliation":[{"name":"Pathology Department, College of Medicine, Zagazig University, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"EL-SAYED HASSAN","sequence":"additional","affiliation":[{"name":"Medical Physiology Department, College of Medicine, Zagazig University, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"19169","published-online":{"date-parts":[[2022,10,31]]},"reference":[{"key":"ref0","doi-asserted-by":"publisher","unstructured":"1. 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