{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T09:43:49Z","timestamp":1780393429552,"version":"3.54.1"},"reference-count":49,"publisher":"Institute of Physiology of the Czech Academy of Sciences","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Physiol Res"],"abstract":"<jats:p>Perinatal hypoxic-ischemic insult (HII) is one of the main devastating causes of morbidity and mortality in newborns. HII induces brain injury which evolves to neurological sequelae later in life. Hypothermia is the only therapeutic approach available capable of diminishing brain impairment after HII. Finding a novel therapeutic method to reduce the severity of brain injury and its consequences is critical in neonatology. The present paper aimed to evaluate the effect of sulforaphane (SFN) pre-treatment on glucose metabolism, neurodegeneration, and functional outcome at the acute, sub-acute, and sub-chronic time intervals in the experimental model of perinatal hypoxic-ischemic insult in rats. To estimate the effect of SFN on brain glucose uptake we have performed 18F-deoxyglucose (FDG) \u03bcCT\/PET. The activity of FDG was determined in the hippocampus and sensorimotor cortex. Neurodegeneration was assessed by histological analysis of Nissl-stained brain sections. To investigate functional outcomes a battery of behavioral tests was employed. We have shown that although SFN possesses a protective effect on glucose uptake in the ischemic hippocampus 24 h and 1 week after HII, no effect has been observed in the motor cortex. We have further shown that the ischemic hippocampal formation tends to be thinner in HIE and SFN treatment tends to reverse this pattern. We have observed subtle chronic movement deficit after HII detected by ladder rung walking test with no protective effect of SFN. SFN should be thus considered as a potent neuroprotective drug with the capability to interfere with pathophysiological processes triggered by perinatal hypoxic-ischemic insult.<\/jats:p>","DOI":"10.33549\/physiolres.934878","type":"journal-article","created":{"date-parts":[[2022,8,8]],"date-time":"2022-08-08T12:14:47Z","timestamp":1659960887000},"page":"401-411","source":"Crossref","is-referenced-by-count":4,"title":["The Effect of Sulforaphane on Perinatal Hypoxic-Ischemic Brain Injury in Rats"],"prefix":"10.33549","author":[{"given":"S","family":"KAPOOR","sequence":"first","affiliation":[{"name":"Centre of Experimental Medicine, Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, Bratislava, Slovak Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D","family":"KALA","sequence":"additional","affiliation":[{"name":"Laboratory of Developmental Epileptology, Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"SVOBODA","sequence":"additional","affiliation":[{"name":"Laboratory of Developmental Epileptology, Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"DAN\u011aK","sequence":"additional","affiliation":[{"name":"Laboratory of Developmental Epileptology, Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"FARIDOV\u00c1","sequence":"additional","affiliation":[{"name":"Laboratory of Developmental Epileptology, Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z","family":"BRNOLIAKOV\u00c1","sequence":"additional","affiliation":[{"name":"Centre of Experimental Medicine, Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, Bratislava, Slovak Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"MIKULECK\u00c1","sequence":"additional","affiliation":[{"name":"Laboratory of Developmental Epileptology, Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"FOLBERGROV\u00c1","sequence":"additional","affiliation":[{"name":"Laboratory of Developmental Epileptology, Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"OT\u00c1HAL","sequence":"additional","affiliation":[{"name":"Laboratory of Developmental Epileptology, Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"19169","published-online":{"date-parts":[[2022,6,30]]},"reference":[{"key":"ref0","doi-asserted-by":"publisher","unstructured":"Allen KA, Brandon DH. Hypoxic ischemic encephalopathy: pathophysiology and experimental treatments. Newborn Infant Nurs Rev 2011;11:125-133. https:\/\/doi.org\/10.1053\/j.nainr.2011.07.004","DOI":"10.1053\/j.nainr.2011.07.004"},{"key":"ref1","doi-asserted-by":"publisher","unstructured":"Bryce J, Boschi-Pinto C, Shibuya K, Black RE, WHO Child Health Epidemiology Reference Group. WHO estimates the causes of death in children. Lancet 2005;365:1147-1152. https:\/\/doi.org\/10.1016\/S0140-6736(05)71877-8","DOI":"10.1016\/s0140-6736(05)71877-8"},{"key":"ref2","doi-asserted-by":"publisher","unstructured":"Edwards AB, Anderton RS, Knuckey NW, Meloni BP. Perinatal hypoxic-ischemic encephalopathy and neuroprotective peptide therapies: A case for cationic arginine-rich peptides (CARPs). Brain Sci 2018;8:147. https:\/\/doi.org\/10.3390\/brainsci8080147","DOI":"10.3390\/brainsci8080147"},{"key":"ref3","doi-asserted-by":"crossref","unstructured":"Glass HC, Shellhaas RA. Acute symptomatic seizures in neonates. Semin Pediatr Neurol 2019;32:100768. https:\/\/doi.org\/10.1016\/j.spen.2019.08.004","DOI":"10.1016\/j.spen.2019.08.004"},{"key":"ref4","doi-asserted-by":"publisher","unstructured":"Varghese B, Xavier R, Manoj VC, Aneesh MK, Priya PS, Ashok Kumar A, Sreenivasan VK. Magnetic resonance imaging spectrum of perinatal hypoxic-ischemic brain injury. Indian J Radiol Imaging 2016;26:316-327. https:\/\/doi.org\/10.4103\/0971-3026.190421","DOI":"10.4103\/0971-3026.190421"},{"key":"ref5","doi-asserted-by":"publisher","unstructured":"Rocha-Ferreira E, Hristova M. Plasticity in the neonatal brain following hypoxic-ischaemic injury. Neural Plast 2016;2016:4901014. https:\/\/doi.org\/10.1155\/2016\/4901014","DOI":"10.1155\/2016\/4901014"},{"key":"ref6","doi-asserted-by":"publisher","unstructured":"Aslam HM Muhammad, Saleem S, Afzal R, Iqbal U, Saleem SM, Abid Shaikh MW, Shahid N. \"Risk factors of birth asphyxia.\" Ital J Pediatr 2014;40:94. https:\/\/doi.org\/10.1186\/s13052-014-0094-2","DOI":"10.1186\/s13052-014-0094-2"},{"key":"ref7","doi-asserted-by":"publisher","unstructured":"Perlman JM. Pathogenesis of hypoxic-ischemic brain injury. J Perinatol 2007;27:S39-S46. https:\/\/doi.org\/10.1038\/sj.jp.7211716","DOI":"10.1038\/sj.jp.7211716"},{"key":"ref8","doi-asserted-by":"crossref","unstructured":"Graham EM, Burd I, Everett AD, Northington FJ. Blood biomarkers for evaluation of perinatal encephalopathy. Front Pharmacol 2016;7:196. https:\/\/doi.org\/10.3389\/fphar.2016.00196","DOI":"10.3389\/fphar.2016.00196"},{"key":"ref9","doi-asserted-by":"publisher","unstructured":"Yang SN, Lai MC. Perinatal hypoxic-ischemic encephalopathy. J Biomed Biotechnol 2011;2011:609813. https:\/\/doi.org\/10.1155\/2011\/609813","DOI":"10.1155\/2011\/609813"},{"key":"ref10","doi-asserted-by":"publisher","unstructured":"Patel M. Mitochondrial dysfunction and oxidative stress: Cause and consequence of epileptic seizures. Free Radic Biol Med 2004;37:1951-1962. https:\/\/doi.org\/10.1016\/j.freeradbiomed.2004.08.021","DOI":"10.1016\/j.freeradbiomed.2004.08.021"},{"key":"ref11","doi-asserted-by":"publisher","unstructured":"Lin MT, Beal MF. Mitochondrial dysfunction and oxidative stress in neurodegenerative diseases. Nature 2006;443:787-795. https:\/\/doi.org\/10.1038\/nature05292","DOI":"10.1038\/nature05292"},{"key":"ref12","doi-asserted-by":"publisher","unstructured":"Rowley S, Patel M. Mitochondrial involvement and oxidative stress in temporal lobe epilepsy. Free Radic Biol Med 2013;62:121-131. https:\/\/doi.org\/10.1016\/j.freeradbiomed.2013.02.002","DOI":"10.1016\/j.freeradbiomed.2013.02.002"},{"key":"ref13","doi-asserted-by":"publisher","unstructured":"Folbergrov\u00e1 J, Je\u0161ina P, Kubov\u00e1 N, Druga R, Ot\u00e1hal J. Status epilepticus in immature rats is associated with oxidative stress and mitochondrial dysfunction. Front Cell Neurosci 2016;10:136. https:\/\/doi.org\/10.3389\/fncel.2016.00136","DOI":"10.3389\/fncel.2016.00136"},{"key":"ref14","doi-asserted-by":"publisher","unstructured":"Bhat AH, Dar KB, Anees S, Zargar MA, Masood A, Sofi MA, Ganie SA. Oxidative stress, mitochondrial dysfunction and neurodegenerative diseases; a mechanistic insight. Biomed Pharmacother 2015;74:101-110. https:\/\/doi.org\/10.1016\/j.biopha.2015.07.025","DOI":"10.1016\/j.biopha.2015.07.025"},{"key":"ref15","doi-asserted-by":"publisher","unstructured":"Qin X, Cheng J, Zhong Y, Mahgoub OK, Akter F, Fan Y, Aldughaim M, Xie Q, Qin L, Gu L, Jian Z, Xiong X, Liu R. Mechanism and treatment related to oxidative stress in neonatal hypoxic-ischemic encephalopathy. Front Mol Neurosci 2019;12:88. https:\/\/doi.org\/10.3389\/fnmol.2019.00088","DOI":"10.3389\/fnmol.2019.00088"},{"key":"ref16","doi-asserted-by":"publisher","unstructured":"Bouayed J, Bohn T. Exogenous antioxidants - Double-edged swords in cellular redox state: Health beneficial effects at physiologic doses versus deleterious effects at high doses. Oxid Med Cell Longev 2010;3:228-237. https:\/\/doi.org\/10.4161\/oxim.3.4.12858","DOI":"10.4161\/oxim.3.4.12858"},{"key":"ref17","doi-asserted-by":"publisher","unstructured":"Mattson MP, Gleichmann M, Cheng A. Mitochondria in neuroplasticity and neurological disorders. Neuron 2008;60:748-766. https:\/\/doi.org\/10.1016\/j.neuron.2008.10.010","DOI":"10.1016\/j.neuron.2008.10.010"},{"key":"ref18","doi-asserted-by":"publisher","unstructured":"Ping Z, Liu W, Kang Z, Cai J, Wang Q, Cheng N, Wang S, Wang S, Zhang JH, Sun X. Sulforaphane protects brains against hypoxic-ischemic injury through induction of Nrf2-dependent phase 2 enzyme. Brain Res 2010;1343:178-185. https:\/\/doi.org\/10.1016\/j.brainres.2010.04.036","DOI":"10.1016\/j.brainres.2010.04.036"},{"key":"ref19","doi-asserted-by":"publisher","unstructured":"Tarozzi A, Angeloni C, Malaguti M, Morroni F, Hrelia S, Hrelia P. Sulforaphane as a potential protective phytochemical against neurodegenerative diseases. Oxid Med Cell Longev 2013;2013:415078. https:\/\/doi.org\/10.1155\/2013\/415078","DOI":"10.1155\/2013\/415078"},{"key":"ref20","doi-asserted-by":"publisher","unstructured":"O'Mealey GB, Berry WL, Plafker SM. Sulforaphane is a Nrf2-independent inhibitor of mitochondrial fission. Redox Biol 2017;11:103-110. https:\/\/doi.org\/10.1016\/j.redox.2016.11.007","DOI":"10.1016\/j.redox.2016.11.007"},{"key":"ref21","doi-asserted-by":"publisher","unstructured":"Kubo E, Chhunchha B, Singh P, Sasaki H, Singh DP. Sulforaphane reactivates cellular antioxidant defense by inducing Nrf2\/ARE\/Prdx6 activity during aging and oxidative stress. Sci Rep 2017;7:14130. https:\/\/doi.org\/10.1038\/s41598-017-14520-8","DOI":"10.1038\/s41598-017-14520-8"},{"key":"ref22","doi-asserted-by":"publisher","unstructured":"Heiss EH, Schachner D, Zimmermann K, Dirsch VM. Glucose availability is a decisive factor for Nrf2-mediated gene expression. Redox Biol 2013;1:359-365. https:\/\/doi.org\/10.1016\/j.redox.2013.06.001","DOI":"10.1016\/j.redox.2013.06.001"},{"key":"ref23","doi-asserted-by":"publisher","unstructured":"Ma Q. Role of Nrf2 in oxidative stress and toxicity. Ann Rev Pharmacol Toxicol 2013;53:401-426. https:\/\/doi.org\/10.1146\/annurev-pharmtox-011112-140320","DOI":"10.1146\/annurev-pharmtox-011112-140320"},{"key":"ref24","doi-asserted-by":"publisher","unstructured":"Tonelli C, Chio IIC, Tuveson DA. Transcriptional regulation by Nrf2. Antioxid Redox Signal 2018;29:1727-1745. https:\/\/doi.org\/10.1089\/ars.2017.7342","DOI":"10.1089\/ars.2017.7342"},{"key":"ref25","doi-asserted-by":"publisher","unstructured":"Zhang Y, Gilmour A, Ahn Y-H, de la Vega L, Dinkova-Kostova AT. The isothiocyanate sulforaphane inhibits mTOR in an NRF2-independent manner. Phytomedicine 2021;86:153062. https:\/\/doi.org\/10.1016\/j.phymed.2019.153062","DOI":"10.1016\/j.phymed.2019.153062"},{"key":"ref26","doi-asserted-by":"publisher","unstructured":"Alfieri A, Srivastava S, Siow RCM, Modo M, Fraser PA, Giovanni E. Targeting the Nrf2-Keap1 antioxidant defence pathway for neurovascular protection in stroke. J Physiol 2011;17:4125-4136. https:\/\/doi.org\/10.1113\/jphysiol.2011.210294","DOI":"10.1113\/jphysiol.2011.210294"},{"key":"ref27","doi-asserted-by":"publisher","unstructured":"Pfister RH, Soll RF. Hypothermia for the treatment of infants with hypoxic-ischemic encephalopathy. J Perinatol 2010;30:82-87. https:\/\/doi.org\/10.1038\/jp.2010.91","DOI":"10.1038\/jp.2010.91"},{"key":"ref28","doi-asserted-by":"publisher","unstructured":"Shah PS, Ohlsson A, Perlman M. Hypothermia to treat neonatal hypoxic ischemic encephalopathy: Systematic review. Obst Gynecol Survey 2008;63:85-86. https:\/\/doi.org\/10.1097\/01.ogx.0000300470.41760.3b","DOI":"10.1097\/01.ogx.0000300470.41760.3b"},{"key":"ref29","doi-asserted-by":"publisher","unstructured":"Frajewicki A, La\u0161t\u016fvka Z, Borb\u00e9lyov\u00e1 V, Khan S, Jandov\u00e1 K, Jani\u0161ov\u00e1 K, Ot\u00e1hal J, Myslive\u010dek J, Riljak V. Perinatal hypoxic-ischemic damage: review of the current treatment possibilities. Physiol Res 2021;69(Suppl 3):S379-S401. https:\/\/doi.org\/10.33549\/physiolres.934595","DOI":"10.33549\/physiolres.934595"},{"key":"ref30","doi-asserted-by":"publisher","unstructured":"Semple BD, Blomgren K, Gimlin K, Ferriero DM, Noble-Haeusslein LJ. Brain development in rodents and humans: Identifying benchmarks of maturation and vulnerability to injury across species. Prog Neurobiol 2013;106-107:1-16. https:\/\/doi.org\/10.1016\/j.pneurobio.2013.04.001","DOI":"10.1016\/j.pneurobio.2013.04.001"},{"key":"ref31","doi-asserted-by":"publisher","unstructured":"Vannucci RC, Vannucci SJ. Perinatal hypoxic-ischemic brain damage: Evolution of an animal model. Dev Neurosci 2005;27:81-86. https:\/\/doi.org\/10.1159\/000085978","DOI":"10.1159\/000085978"},{"key":"ref32","unstructured":"Sarikaya I. PET studies in epilepsy. Am J Nucl Med Mol Imaging 2015;5:416-430."},{"key":"ref33","doi-asserted-by":"publisher","unstructured":"Schiffer WK, Mirrione MM, Biegon A, Alexoff DL, Patel V, Dewey SL. Serial microPET measures of the metabolic reaction to a microdialysis probe implant. J Neurosci Methods 2006;155:272-284. https:\/\/doi.org\/10.1016\/j.jneumeth.2006.01.027","DOI":"10.1016\/j.jneumeth.2006.01.027"},{"key":"ref34","doi-asserted-by":"publisher","unstructured":"S\u00e1nchez F, Orero A, Soriano A. ALBIRA: A small animal PET\/SPECT\/CT imaging system. Med Phys 2013;40:051906. https:\/\/doi.org\/10.1118\/1.4800798","DOI":"10.1118\/1.4800798"},{"key":"ref35","doi-asserted-by":"publisher","unstructured":"Metz GA, Whishaw IQ. The ladder rung walking task: a scoring system and its practical application. J Vis Exp 2009;(28):1204. https:\/\/doi.org\/10.3791\/1204","DOI":"10.3791\/1204"},{"key":"ref36","doi-asserted-by":"publisher","unstructured":"Dinkova-Kostova AT, Kostov RV. Glucosinolates and isothiocyanates in health and disease. Trends Mol Med 2012;18:337-347. https:\/\/doi.org\/10.1016\/j.molmed.2012.04.003","DOI":"10.1016\/j.molmed.2012.04.003"},{"key":"ref37","doi-asserted-by":"publisher","unstructured":"De Lange C, Malinen E, Qu H, Johnsrud K, Skretting A, Saugstad OD, Munkeby BH. Dynamic FDG PET for assessing early effects of cerebral hypoxia and resuscitation in new-born pigs. Eur J Nucl Med Mol Imaging 2012;39:792-799. https:\/\/doi.org\/10.1007\/s00259-011-2055-y","DOI":"10.1007\/s00259-011-2055-y"},{"key":"ref38","doi-asserted-by":"publisher","unstructured":"Gilland E, Bona E, Hagberg H. Temporal changes of regional glucose use, blood flow, and microtubule- associated protein 2 immunostaining after hypoxia-ischemia in the immature rat brain. J Cereb Blood Flow Metab 1998;18:222-228. https:\/\/doi.org\/10.1097\/00004647-199802000-00014","DOI":"10.1097\/00004647-199802000-00014"},{"key":"ref39","doi-asserted-by":"publisher","unstructured":"Vannucci RC, Yager JY, Vannucci SJ. Cerebral glucose and energy utilization during the evolution of hypoxic- ischemic brain damage in the immature rat. J Cereb Blood Flow Metab 1994;14:279-288. https:\/\/doi.org\/10.1038\/jcbfm.1994.35","DOI":"10.1038\/jcbfm.1994.35"},{"key":"ref40","doi-asserted-by":"publisher","unstructured":"Thorngren-Jerneck K, Ohlsson T, Sandell A, Erlandsson K, Strand SE, Ryding E, Svenningsen NW. Cerebral glucose metabolism measured by positron emission tomography in term newborn infants with hypoxic ischemic encephalopathy. Pediatr Res 2001;49:495-501. https:\/\/doi.org\/10.1203\/00006450-200104000-00010","DOI":"10.1203\/00006450-200104000-00010"},{"key":"ref41","doi-asserted-by":"publisher","unstructured":"Svoboda J, Litvinec A, Kala D, Po\u0161usta A, V\u00e1vrov\u00e1 L, Jiru\u0161ka P, Ot\u00e1hal J. Strain differences in intraluminal thread model of middle cerebral artery occlusion in rats. Physiol Res 2019;68:37-48. https:\/\/doi.org\/10.33549\/physiolres.933958","DOI":"10.33549\/physiolres.933958"},{"key":"ref42","doi-asserted-by":"publisher","unstructured":"La\u0161t\u016fvka Z, Borb\u00e9lyov\u00e1 V, Jani\u0161ov\u00e1 K, Ot\u00e1hal J, Myslive\u010dek J, Riljak V. Neonatal hypoxic-ischemic brain injury leads to sex-specific deficits in rearing and climbing in adult mice. Physiol Res 2020;69(Suppl 3):S499-S512. https:\/\/doi.org\/10.33549\/physiolres.934604","DOI":"10.33549\/physiolres.934604"},{"key":"ref43","doi-asserted-by":"publisher","unstructured":"Lubics A, Reglodi D, Tam\u00e1s A, Kiss P, Szalai M, Szalontay L, Lengv\u00e1ri I. Neurological reflexes and early motor behavior in rats subjected to neonatal hypoxic-ischemic injury. Behav Brain Res 2005;157:157-165. https:\/\/doi.org\/10.1016\/j.bbr.2004.06.019","DOI":"10.1016\/j.bbr.2004.06.019"},{"key":"ref44","doi-asserted-by":"publisher","unstructured":"Goodfellow MJ, Borcar A, Proctor JL, Greco T, Rosenthal RE, Fiskum G. Transcriptional activation of antioxidant gene expression by Nrf2 protects against mitochondrial dysfunction and neuronal death associated with acute and chronic neurodegeneration. Exp Neurol 2020;328:113247. https:\/\/doi.org\/10.1016\/j.expneurol.2020.113247","DOI":"10.1016\/j.expneurol.2020.113247"},{"key":"ref45","doi-asserted-by":"publisher","unstructured":"Guerrero-Beltr\u00e1n CE, Calder\u00f3n-Oliver M, Pedraza-Chaverri J, Chirino YI. Protective effect of sulforaphane against oxidative stress: recent advances. Exp Toxicol Pathol 2012;64:503-508. https:\/\/doi.org\/10.1016\/j.etp.2010.11.005","DOI":"10.1016\/j.etp.2010.11.005"},{"key":"ref46","unstructured":"Ma LL, Xing GP, Yu Y, Liang H, Yu T-X, Zheng W-H, Lai T-B. Exerts neuroprotective effects via suppression of the inflammatory response in a rat model of focal cerebral ischemia. Int J Clin Exp Med 2015;8:17811-17817."},{"key":"ref47","doi-asserted-by":"publisher","unstructured":"Zhao X, Sun G, Zhang J, Strong R, Dash PK, KanYW, Grotta JC, Aronowski J. Transcription factor Nrf2 protects the brain from damage produced by intracerebral hemorrhage. Stroke 2007;38:3280-3286. https:\/\/doi.org\/10.1161\/STROKEAHA.107.486506","DOI":"10.1161\/strokeaha.107.486506"},{"key":"ref48","doi-asserted-by":"publisher","unstructured":"Dan\u011bk J, Dana\u010d\u00edkov\u00e1 \u0160, Kala D, Svoboda J, Kapoor S, Kapoor S, Po\u0161usta A, Folbergrov\u00e1 J, Tauchmannov\u00e1 K, Mr\u00e1\u010dek T, Ot\u00e1hal J. Sulforaphane ameliorates metabolic changes associated with status epilepticus in immature rats. Front Cell Neurosci 2022;16:855161. https:\/\/doi.org\/10.3389\/fncel.2022.855161","DOI":"10.3389\/fncel.2022.855161"}],"container-title":["Physiological Research"],"original-title":[],"language":"en","deposited":{"date-parts":[[2022,8,8]],"date-time":"2022-08-08T12:14:52Z","timestamp":1659960892000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.biomed.cas.cz\/physiolres\/pdf\/2022\/71_401.pdf"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,30]]},"references-count":49,"URL":"https:\/\/doi.org\/10.33549\/physiolres.934878","relation":{},"ISSN":["1802-9973","0862-8408"],"issn-type":[{"value":"1802-9973","type":"electronic"},{"value":"0862-8408","type":"print"}],"subject":[],"published":{"date-parts":[[2022,6,30]]}}}