{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T08:31:28Z","timestamp":1722933088258},"reference-count":29,"publisher":"Institute of Physiology of the Czech Academy of Sciences","issue":"Suppl. 1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Physiol Res"],"published-print":{"date-parts":[[2022,12,27]]},"abstract":"<jats:p>Uterine tubes (UTs) are essential during physiological reproduction. The most intriguing part of its wall is the mucosa. Apart from the epithelial cells vital for its normal function, the connective tissue lamina propria contains wide spaces whose function, morphology and structure are yet to be elucidated. The present study used bioptic samples from 25 premenopausal (mean age 48.3 years, \u03c3=3.56) and 25 postmenopausal women (mean age 57.8 years, \u03c3=7.79). In both study groups, samples were obtained from two anatomically distinct parts of the UT \u2013 ampulla and infundibulum with fimbriae. The specimens were processed for scanning electron microscopy (SEM) and immunohistochemical detection of podoplanin (clone D2-40) and VEGFR-3 \u2013 two markers of lymphatic endothelial cells. The results showed that specimens from premenopausal and postmenopausal women contain wide lymphatic spaces, also known as lymphatic lacunae. The most probable function of the lacunae in the fimbriae is oocyte pick-up upon ovulation thanks to their ability to get engorged with lymph, thus serving as an erectile-like tissue. The ampullary lacunae are probably responsible for tubal fluid maintenance and recirculation. These results indicate that they are vital for normal reproduction because tubal fluid dynamics are as important as fluid composition. Further research on this topic is highly warranted because more detailed insights into UT function have a great potential to refine the methods of reproductive medicine, e.g. in vitro fertilization (IVF), which are still far from optimal regarding fertility outcomes.<\/jats:p>","DOI":"10.33549\/physiolres.935029","type":"journal-article","created":{"date-parts":[[2022,12,30]],"date-time":"2022-12-30T20:23:33Z","timestamp":1672431813000},"page":"S115-S123","source":"Crossref","is-referenced-by-count":2,"title":["Immunohistochemical and Scanning Electron Microscopic Confirmation of the Lymphatic Lacunae in the Uterine Tube Mucosal Folds. What Are the Clinical Implications?"],"prefix":"10.33549","volume":"71","author":[{"given":"M","family":"Cs\u00f6b\u00f6nyeiov\u00e1","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Klein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Jur\u00edkov\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Feitscherov\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P","family":"G\u00e1lfiov\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I","family":"Varga","sequence":"additional","affiliation":[{"name":"Institute of Histology and Embryology, Faculty of Medicine, Comenius University in Bratislava, Slovakia. ivan.varga@fmed.uniba.sk"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"19169","reference":[{"key":"ref0","doi-asserted-by":"crossref","unstructured":"Eddy CA, Pauerstein CJ. Anatomy and physiology of the fallopian tube. Clin Obstet Gynecol 1980;23:1177-1193. https:\/\/doi.org\/10.1097\/00003081-198012000-00023","DOI":"10.1097\/00003081-198012000-00023"},{"key":"ref1","doi-asserted-by":"publisher","unstructured":"Tiourin E, Velasco VS, Rosales MA, Sullivan PS, Janzen DM, Memarzadeh S. Tubal Ligation Induces Quiescence in the Epithelia of the Fallopian Tube Fimbria. Reprod Sci 2015;22:1262-1271. https:\/\/doi.org\/10.1177\/1933719115574345","DOI":"10.1177\/1933719115574345"},{"key":"ref2","unstructured":"Gartner LP. Color Atlas and Text of Histology. Wolters Kluwer; 2018."},{"key":"ref3","unstructured":"Mills SE. (Ed). Histology for Pathologists. Fifth Edition. Wolters Kluwer Health; 2020."},{"key":"ref4","unstructured":"Young B, O'Dowd G, Woodford P. Wheater's Functional Histology: A Text and Colour Atlas. Sixth Edition. Elsevier Churchill Livingstone; 2014."},{"key":"ref5","doi-asserted-by":"publisher","unstructured":"Varga I, Miko M, Kachl\u00edk D, \u017di\u0161kov\u00e1 M, Danihel \u013d, Jr., Bab\u00e1l P. How many cell types form the epithelial lining of the human uterine tubes? Revision of the histological nomenclature of the human tubal epithelium. Ann Anat 2019;224:73-80. https:\/\/doi.org\/10.1016\/j.aanat.2019.03.012","DOI":"10.1016\/j.aanat.2019.03.012"},{"key":"ref6","doi-asserted-by":"crossref","unstructured":"Mastroianni L, Jr. The fallopian tube and reproductive health. J Pediatr Adolesc Gynecol 1999;12:121-126. https:\/\/doi.org\/10.1016\/S1038-3188(99)00003-0","DOI":"10.1016\/S1038-3188(99)00003-0"},{"key":"ref7","unstructured":"Ascher E, Madelenat P, Rose D. [Tubal physiology: structures and functions]. J Gynecol Obstet Biol Reprod (Paris) 1986;15:717-729."},{"key":"ref8","unstructured":"Kajanov\u00e1 M, L D, S P, Miko M, Urban L, Bokor T, Varga I. [The structural basis for transport through the Fallopian tube]. Ceska Gynekol 2012;77:566-571."},{"key":"ref9","doi-asserted-by":"publisher","unstructured":"Kroemer P. Die Lymphorgane der weiblichen Genitalien und ihre Ver\u00e4n-derungen bei malignen Erkrankungen des Uterus. Habilitationsschrift. Arch Gyn\u00e4k 1904;73:1-102. https:\/\/doi.org\/10.1007\/BF01670168","DOI":"10.1007\/bf01670168"},{"key":"ref10","doi-asserted-by":"publisher","unstructured":"Varga I, Kachl\u00edk D, \u017di\u0161kov\u00e1 M, Miko M. Lymphatic lacunae of the mucosal folds of human uterine tubes \u2013 A rediscovery of forgotten structures and their possible role in reproduction. Ann Anat 2018;219:121-128. https:\/\/doi.org\/10.1016\/j.aanat.2018.06.005","DOI":"10.1016\/j.aanat.2018.06.005"},{"key":"ref11","unstructured":"Ozdowski L, Gupta V. Physiology, Lymphatic System. StatPearls. StatPearls Publishing, StatPearls Publishing LLC.; 2022."},{"key":"ref12","doi-asserted-by":"publisher","unstructured":"Jansen RP. Cyclic changes in the human fallopian tube isthmus and their functional importance. Am J Obstet Gynecol 1980;136:292-308. https:\/\/doi.org\/10.1016\/0002-9378(80)90853-4","DOI":"10.1016\/0002-9378(80)90853-4"},{"key":"ref13","doi-asserted-by":"publisher","unstructured":"Folpe AL, Veikkola T, Valtola R, Weiss SW. Vascular endothelial growth factor receptor-3 (VEGFR-3): a marker of vascular tumors with presumed lymphatic differentiation, including Kaposi's sarcoma, kaposiform and Dabska-type hemangioendotheliomas, and a subset of angiosarcomas. Mod Pathol 2000;13:180-185. https:\/\/doi.org\/10.1038\/modpathol.3880033","DOI":"10.1038\/modpathol.3880033"},{"key":"ref14","doi-asserted-by":"publisher","unstructured":"Kahn HJ, Bailey D, Marks A. Monoclonal antibody D2-40, a new marker of lymphatic endothelium, reacts with Kaposi's sarcoma and a subset of angiosarcomas. Mod Pathol 2002;15:434-440. https:\/\/doi.org\/10.1038\/modpathol.3880543","DOI":"10.1038\/modpathol.3880543"},{"key":"ref15","doi-asserted-by":"publisher","unstructured":"Kachlik D, Musil V, Baca V. Terminologia Anatomica after 17 years: inconsistencies, mistakes and new proposals. Ann Anat 2015;201:8-16. https:\/\/doi.org\/10.1016\/j.aanat.2015.04.006","DOI":"10.1016\/j.aanat.2015.04.006"},{"key":"ref16","unstructured":"Kierszenbaum AL, Tres LL. Histology and Cell Biology: An Introduction to Pathology E-Book. Elsevier Health Sciences; 2019."},{"key":"ref17","unstructured":"Eroschenko VP, di Fiore MSH. DiFiore's Atlas of Histology with Functional Correlations. Wolters Kluwer Health\/Lippincott Williams & Wilkins; 2013."},{"key":"ref18","doi-asserted-by":"publisher","unstructured":"Mescher A. Junqueira's Basic Histology: Text and Atlas 14th Edition. McGraw-Hill Education; 2015.","DOI":"10.26641\/1997-9665.2016.4.102"},{"key":"ref19","doi-asserted-by":"publisher","unstructured":"Labidi-Galy SI, Papp E, Hallberg D, Niknafs N, Adleff V, Noe M, Bhattacharya R, Novak M, Jones S, Phallen J, Hruban CA, Hirsch MS, Lin DI, Schwartz L, Maire CL, Tille JC, Bowden M, Ayhan A, Wood LD, Scharpf RB, Kurman R, Wang TL, Shih IM, Karchin R, Drapkin R, Velculescu VE. High grade serous ovarian carcinomas originate in the fallopian tube. Nat Commun 2017;8:1093. https:\/\/doi.org\/10.1038\/s41467-017-00962-1","DOI":"10.1038\/s41467-017-00962-1"},{"key":"ref20","doi-asserted-by":"publisher","unstructured":"Saint-Dizier M, Schoen J, Chen S, Banliat C, Mermillod P. Composing the early embryonic microenvironment: physiology and regulation of oviductal secretions. Int J Mol Sci 2019;21:223. https:\/\/doi.org\/10.3390\/ijms21010223","DOI":"10.3390\/ijms21010223"},{"key":"ref21","doi-asserted-by":"publisher","unstructured":"Ferraz M, Rho HS, Hemerich D, Henning HHW, van Tol HTA, H\u00f6lker M, Besenfelder U, Mokry M, Vos P, Stout TAE, Le Gac S, Gadella BM. An oviduct-on-a-chip provides an enhanced in vitro environment for zygote genome reprogramming. Nat Commun 2018;9:4934. https:\/\/doi.org\/10.1038\/s41467-018-07119-8","DOI":"10.1038\/s41467-018-07119-8"},{"key":"ref22","doi-asserted-by":"publisher","unstructured":"Wang M, Zhu T, Liu C, Jin L, Fei P, Zhang B. Oviduct-mimicking microfluidic chips decreased the ROS concentration in the in vitro fertilized embryos of CD-1 mice. Biomed Pharmacother 2022;154:113567. https:\/\/doi.org\/10.1016\/j.biopha.2022.113567","DOI":"10.1016\/j.biopha.2022.113567"},{"key":"ref23","doi-asserted-by":"publisher","unstructured":"Otsuki Y, Maeda Y, Magari S, Sugimoto O. Lymphatics and lymphoid tissue of the fallopian tube: immunoelectronmicroscopic study. Anat Rec 1989;225:288-296. https:\/\/doi.org\/10.1002\/ar.1092250405","DOI":"10.1002\/ar.1092250405"},{"key":"ref24","doi-asserted-by":"publisher","unstructured":"LeBlanc MM, Johnson RD, Calderwood Mays MB, Valderrama C. Lymphatic clearance of India ink in reproductively normal mares and mares susceptible to endometritis. Biol Reprod 1995;52:501-506. https:\/\/doi.org\/10.1093\/biolreprod\/52.monograph_series1.501","DOI":"10.1093\/biolreprod\/52.monograph_series1.501"},{"key":"ref25","doi-asserted-by":"publisher","unstructured":"Red-Horse K. Lymphatic vessel dynamics in the uterine wall. Placenta 2008;29 (Suppl A):S55-59. https:\/\/doi.org\/10.1016\/j.placenta.2007.11.011","DOI":"10.1016\/j.placenta.2007.11.011"},{"key":"ref26","doi-asserted-by":"publisher","unstructured":"Nicolescu MI, Rusu MC, Voinea LM, Vrapciu AD, B\u00e2r\u0103 RI. Lymphatic lacunae of the human eye conjunctiva embedded within a stroma containing CD34(+) telocytes. J Cell Mol Med 2020;24:8871-8875. https:\/\/doi.org\/10.1111\/jcmm.15354","DOI":"10.1111\/jcmm.15354"},{"key":"ref27","doi-asserted-by":"publisher","unstructured":"Zurzu M, Nicolescu MI, Mogoant\u0103 L, Pantea S, Rusu MC. Telocytes and lymphatics of the human colon. Life (Basel) 2021; 11(10):1001. https:\/\/doi.org\/10.3390\/life11101001","DOI":"10.3390\/life11101001"},{"key":"ref28","doi-asserted-by":"publisher","unstructured":"Li J, Zhao Z, Zhou J, Yu S. A study of the three-dimensional organization of the human diaphragmatic lymphatic lacunae and lymphatic drainage units. 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