{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T10:46:50Z","timestamp":1787222810175,"version":"build-2736575974"},"reference-count":65,"publisher":"Institute of Physiology of the Czech Academy of Sciences","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Physiol Res"],"abstract":"<jats:p>Waterpipe tobacco smoking (WPS) inhalation has been shown to trigger endothelial dysfunction and atherosclerosis. However, the mechanisms underlying these effects are still unknown. Here, we assessed the impact and underlying mechanism of WPS exposure for one month on endothelial dysfunction using aortic tissue of mice. The duration of the session was 30 min\/day and 5 days\/week. Control mice were exposed to air. Inhalation of WPS induced an increase in the number of macrophages and neutrophils and the concentrations of protein, tumor necrosis factor \u03b1 (TNF \u03b1), interleukin (IL)-1\u03b2, and glutathione in bronchoalveolar lavage fluid. Moreover, the concentrations of proinflammatory cytokines (TNF \u03b1, IL-6 and IL-1\u03b2), adhesion molecules (intercellular adhesion molecule-1, vascular cell adhesion molecule-1, E-selectin and P-selectin) and markers of oxidative stress (lipid peroxidation, glutathione, superoxide dismutase and nitric oxide) in aortic homogenates of mice exposed to WPS were significantly augmented compared with air exposed mice. Likewise, the concentration of galectin-3 was significantly increased in the aortic homogenates of mice exposed to WPS compared with control group. WPS inhalation induced vascular DNA damage assessed by comet assay and apoptosis characterized by a significant increase in cleaved caspase-3. While the aortic expression of phosphorylated nuclear factor \u03baB (NF-\u03baB) was significantly increased following WPS inhalation, the concentration of sirtuin 1 (SIRT1) was significantly decreased in WPS group compared with air-exposed group. In conclusion, our study provided evidence that WPS inhalation triggers lung injury and endothelial inflammation, oxidative stress and apoptosis which were associated with nuclear factor-\u03baB activation and SIRT1 down-regulation.<\/jats:p>","DOI":"10.33549\/physiolres.935042","type":"journal-article","created":{"date-parts":[[2023,7,13]],"date-time":"2023-07-13T14:08:54Z","timestamp":1689257334000},"source":"Crossref","is-referenced-by-count":10,"title":["Waterpipe Smoke Inhalation Induces Lung Injury and Aortic Endothelial Dysfunction in Mice"],"prefix":"10.33549","volume":"72","author":[{"given":"A","family":"Nemmar","sequence":"first","affiliation":[{"name":"Departments of Physiology, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Beegam","sequence":"additional","affiliation":[{"name":"Departments of Physiology, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N","family":"Zaaba","sequence":"additional","affiliation":[{"name":"Departments of Physiology, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O","family":"Elzaki","sequence":"additional","affiliation":[{"name":"Departments of Physiology, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Pathan","sequence":"additional","affiliation":[{"name":"Departments of Physiology, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B","family":"Ali","sequence":"additional","affiliation":[{"name":"Independent Researcher, Lincoln, Nebraska, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"19169","published-online":{"date-parts":[[2023,6,30]]},"reference":[{"key":"ref0","doi-asserted-by":"publisher","unstructured":"1.\tRababa'h AM, Mardini AN, Ababneh MA, Alzoubi KH: Waterpipe tobacco smoke and health: What we have learned from rodent models? 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