{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,29]],"date-time":"2025-05-29T09:53:08Z","timestamp":1748512388108},"reference-count":23,"publisher":"Institute of Physiology of the Czech Academy of Sciences","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Physiol Res"],"published-print":{"date-parts":[[2023,12,17]]},"abstract":"<jats:p>Spontaneous preterm birth (sPTB) is a major cause of perinatal morbidity and mortality, even in developed countries. Prediction of sPTB is therefore a valuable tool to reduce the associated risks. The current standard for the prediction of sPTB consists, in addition to anamnestic data, of previous sPTB and previous second trimester miscarriage, measurement of cervical length by transvaginal ultrasound (TVU CL) together with assessment of fetal fibronectin levels in cervicovaginal fluid. Other evaluation parameters, such as the level of endocannabinoids in the pregnant woman's blood, could increase the sensitivity of this management. Endocannabinoids (eCBs) are a part of the endocannabinoid system (ECS); out of them anandamide (arachidonoyl-ethanolamide, AEA), in particular, plays an important role in the regulation of pregnancy and childbirth. We present the protocol for an open, non-randomized study to evaluate concentrations of AEA and other endocannabinoids: 2 linoleoylglycerol (2-AG), 2 linoleoylglycerol (2-LG), 2 oleoylglycerol (2-OG), and 2 arachidonoyldopamine (2-ADOPA or also NADA) in the blood of pregnant women as potential predictors of sPTB. In a total of 230 women with a history of sPTB or miscarriage, eCBs levels between 22 and 28 weeks of gestation will be assessed from maternal blood, in addition to the standard procedure. The aim of the study is to determine the relationship between blood concentrations of the endocannabinoids tested and the risk of sPTB. The results of this study will describe the prognostic significance of maternal blood eCBs levels for sPTB, and could subsequently enable improved screening programs for early identification of sPTB.<\/jats:p>","DOI":"10.33549\/physiolres.935230","type":"journal-article","created":{"date-parts":[[2023,12,19]],"date-time":"2023-12-19T14:16:17Z","timestamp":1702995377000},"page":"S381-S387","source":"Crossref","is-referenced-by-count":1,"title":["The Endocannabinoid System - The Prediction of Spontaneous Preterm Birth in High-Risk Women: Protocol of a Study"],"prefix":"10.33549","author":[{"given":"A","family":"Pa\u0159\u00edzek","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Hill","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Du\u0161kov\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L","family":"Kol\u00e1torov\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Suchop\u00e1r","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P","family":"\u0160imj\u00e1k","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Anderlov\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E","family":"Kudov\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V","family":"Rogalewicz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Vacek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Koudelkov\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Chloupkov\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Alblov\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P","family":"Pa\u0159\u00edzkov\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Z","family":"La\u0161t\u016fvka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Bart\u00e1k","sequence":"additional","affiliation":[{"name":"First Faculty of Medicine, Charles University, Prague, Czech Republic. miroslav.bartak@lf1.cuni.cz"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"19169","reference":[{"key":"ref0","unstructured":"WHO Preterm birth. Acessed 30. 8. 2023 https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/preterm-birth"},{"key":"ref1","doi-asserted-by":"publisher","unstructured":"Velebil P. 2022. Results of perinatal care in the Czech Republic in 2019. Accessed 30. 8. 2023. https:\/\/www.mzcr.cz\/wp-content\/uploads\/2020\/07\/V %C3 %BDsledky-perinat %C3 %A1ln %C3 %AD-p %C3 %A9 %C4 %8De-za-rok-2019.pdf","DOI":"10.31692\/2358-9728.vcointerpdvl.2018.00085"},{"key":"ref2","doi-asserted-by":"publisher","unstructured":"Aung MT, Yu Y, Ferguson KK, et al. Prediction and associations of preterm birth and its subtypes with eicosanoid enzymatic pathways and inflammatory markers. Scientific Reports. 2019;9(1). https:\/\/doi.org\/10.1038\/s41598-019-53448-z","DOI":"10.1038\/s41598-019-53448-z"},{"key":"ref3","doi-asserted-by":"publisher","unstructured":"Kozakiewicz ML, Grotegut CA, Howlett AC. Endocannabinoid system in pregnancy maintenance and labor: A mini-review. Front Endocrinol 2021;12. https:\/\/doi.org\/10.3389\/fendo.2021.699951","DOI":"10.3389\/fendo.2021.699951"},{"key":"ref4","doi-asserted-by":"publisher","unstructured":"Ezechukwu HC, Diya CA, Shrestha N, Hryciw DH. Role for endocannabinoids in early pregnancy: Recent advances and the effects of cannabis use. Am J Physiol-Endocrinol Metabol 2020;319: E557-E561. https:\/\/doi.org\/10.1152\/ajpendo.00210.2020","DOI":"10.1152\/ajpendo.00210.2020"},{"key":"ref5","doi-asserted-by":"publisher","unstructured":"Mart\u00ednez-Pe\u00f1a AA, Perono GA, Gritis SA, et al. The impact of early life exposure to cannabis: The role of the endocannabinoid system. Int J Mol Sci 2021;22:8576. https:\/\/doi.org\/10.3390\/ijms22168576","DOI":"10.3390\/ijms22168576"},{"key":"ref6","doi-asserted-by":"publisher","unstructured":"Maia J, Fonseca B, Teixeira N, Correia-da-Silva G. The fundamental role of the endocannabinoid system in endometrium and placenta: Implications in pathophysiological aspects of uterine and pregnancy disorders. Human Reproduction Update. 2020;26:586-602. https:\/\/doi.org\/10.1093\/humupd\/dmaa005","DOI":"10.1093\/humupd\/dmaa005"},{"key":"ref7","doi-asserted-by":"publisher","unstructured":"Bachkangi P, Taylor AH, Bari M, Maccarrone M, Konje JC. Prediction of preterm labour from a single blood test: The role of the endocannabinoid system in predicting preterm birth in high-risk women. Eur J Obst Gynecol Reprod Biol 2019;243:1-6. https:\/\/doi.org\/10.1016\/j.ejogrb.2019.09.029","DOI":"10.1016\/j.ejogrb.2019.09.029"},{"key":"ref8","doi-asserted-by":"publisher","unstructured":"La\u0161t\u016fvka Z, Suchop\u00e1r J, Ma\u0161kov\u00e1 S, Alblov\u00e1 M, Pa\u0159\u00edzek A. The role of endocannabinoids in pregnancy. \u010cesk\u00e1 gynekologie. 2021;86:349-354. https:\/\/doi.org\/10.48095\/cccg2021349","DOI":"10.48095\/cccg2021349"},{"key":"ref9","unstructured":"Bachkangi, P. The Role of the Endocannabinoid System in Preterm Birth. University of Leicester, 2018. Accessed 30. 8. 2023. https:\/\/hdl.handle.net\/2381\/42773"},{"key":"ref10","doi-asserted-by":"publisher","unstructured":"Hanlon EC. Impact of circadian rhythmicity and sleep restriction on circulating endocannabinoid (eCB) N-arachidonoylethanolamine (anandamide). Psychoneuroendocrinology 2020, 111, 104471. https:\/\/doi.org\/10.1016\/j.psyneuen.2019.104471","DOI":"10.1016\/j.psyneuen.2019.104471"},{"key":"ref11","doi-asserted-by":"publisher","unstructured":"Hanlon EC, Tasali E, Leproult R, Stuhr KL, Doncheck E, de Wit H, Hillard CJ, Van Cauter E. Circadian rhythm of circulating levels of the endocannabinoid 2-arachidonoylglycerol.  J Clin Endocrinol Metab 2015;100:220-226. https:\/\/doi.org\/10.1210\/jc.2014-3455","DOI":"10.1210\/jc.2014-3455"},{"key":"ref12","doi-asserted-by":"publisher","unstructured":"Burchard J, Markenson GR, Saade GR, et al. Clinical and economic evaluation of a proteomic biomarker preterm birth risk predictor: Cost-effectiveness modeling of prenatal interventions applied to predicted higher-risk pregnancies within a large and diverse cohort. J Med Econom 2022;25:1255-1266. https:\/\/doi.org\/10.1080\/13696998.2022.2147771","DOI":"10.1080\/13696998.2022.2147771"},{"key":"ref13","doi-asserted-by":"publisher","unstructured":"Wong K, Tessema GA, Chai K, Pereira G. Development of prognostic model for preterm birth using machine learning in a population-based cohort of Western Australia births between 1980 and 2015. Scientific Reports 2022;12. https:\/\/doi.org\/10.1038\/s41598-022-23782-w","DOI":"10.1038\/s41598-022-23782-w"},{"key":"ref14","doi-asserted-by":"publisher","unstructured":"Ruma MS, Banker WM. Availability and use of fetal fibronectin testing and transvaginal ultrasound for preterm labor evaluation in the United States. The Journal of Maternal-Fetal &amp; Neonatal Medicine 2021;35:8586-8593. https:\/\/doi.org\/10.1080\/14767058.2021.1989403","DOI":"10.1080\/14767058.2021.1989403"},{"key":"ref15","doi-asserted-by":"publisher","unstructured":"Dumlao DS, Buczynski MW, Norris PC, Harkewicz R, Dennis EA. High-throughput lipidomic analysis of fatty acid derived eicosanoids and N-acylethanolamines. Biochim Biophys Acta 2011;1811:724-736. https:\/\/doi.org\/10.1016\/j.bbalip.2011.06.005","DOI":"10.1016\/j.bbalip.2011.06.005"},{"key":"ref16","doi-asserted-by":"publisher","unstructured":"Adamcov\u00e1 K, Kol\u00e1torov\u00e1 L, \u0160kodov\u00e1 T, \u0160imkov\u00e1 M, Pa\u0159\u00edzek A, St\u00e1rka L, Du\u0161kov\u00e1 M. Steroid hormone levels in the peripartum period - differences caused by fetal sex and delivery type. Physiol Res. 2018;67(Suppl 3):S489-S497. https:\/\/doi.org\/10.33549\/physiolres.934019","DOI":"10.33549\/physiolres.934019"},{"key":"ref17","doi-asserted-by":"publisher","unstructured":"Pa\u0161kov\u00e1 A, Pa\u0159\u00edzek A, Hill M, Vel\u00edkov\u00e1 M, Kub\u00e1tov\u00e1 J, Du\u0161kov\u00e1 M, Adamcov\u00e1 K, Kouck\u00fd M, Simjak P, \u010cern\u00fd A, St\u00e1rka L. Steroid metabolome in the umbilical cord: is it necessary to differentiate between arterial and venous blood? Physiol Res. 2014;63:115-126. https:\/\/doi.org\/10.33549\/physiolres.932624","DOI":"10.33549\/physiolres.932624"},{"key":"ref18","doi-asserted-by":"publisher","unstructured":"Pa\u0159\u00edzek A, Mike\u0161ov\u00e1 M, Jir\u00e1k R, Hill M, Kouck\u00fd M, Pa\u0161kov\u00e1 A, Vel\u00edkov\u00e1 M, Adamcov\u00e1 K, \u0160r\u00e1mkov\u00e1 M, Jand\u00edkov\u00e1 H, Du\u0161kov\u00e1 M, St\u00e1rka L. Steroid hormones in the development of postpartum depression. Physiol Res 2014;63(Suppl 2):S277-S282. https:\/\/doi.org\/10.33549\/physiolres.932788","DOI":"10.33549\/physiolres.932788"},{"key":"ref19","doi-asserted-by":"publisher","unstructured":"Adamcov\u00e1 K, Kol\u00e1torov\u00e1 L, Chlup\u00e1\u010dov\u00e1 T, \u0160imkov\u00e1 M, Jand\u00edkov\u00e1 H, Pa\u0159\u00edzek A, St\u00e1rka L, Du\u0161kov\u00e1 M. Changes to fetal steroidogenesis caused by maternal smoking. Physiol Res 2017;66(Suppl 3):S375-S386.  https:\/\/doi.org\/10.33549\/physiolres.933728","DOI":"10.33549\/physiolres.933728"},{"key":"ref20","doi-asserted-by":"publisher","unstructured":"Bandres-Meriz J, Kunz C, Havelund JF, et al. Distinct maternal metabolites are associated with obesity and glucose-insulin axis in the first trimester of pregnancy. Int j Obesity 2023;47:529-537. https:\/\/doi.org\/10.1038\/s41366-023-01295-4","DOI":"10.1038\/s41366-023-01295-4"},{"key":"ref21","doi-asserted-by":"publisher","unstructured":"Habayeb OM, Taylor AH, Evans MD, et al. Plasma levels of the endocannabinoid anandamide in women-a potential role in pregnancy maintenance and labor? J Clin Endocrinol Metab 2004;89:5482-5487. https:\/\/doi.org\/10.1210\/jc.2004-0681","DOI":"10.1210\/jc.2004-0681"},{"key":"ref22","doi-asserted-by":"publisher","unstructured":"Nallendran V, Lam P, Marczylo T, et al. The plasma levels of the endocannabinoid, anandamide, increase with the induction of labour. BJOG:J Int Obster Gynaecol 2010;117:863-869. https:\/\/doi.org\/10.1111\/j.1471-0528.2010.02555.x","DOI":"10.1111\/j.1471-0528.2010.02555.x"}],"container-title":["Physiological Research"],"original-title":[],"language":"en","deposited":{"date-parts":[[2023,12,19]],"date-time":"2023-12-19T14:16:21Z","timestamp":1702995381000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.biomed.cas.cz\/physiolres\/pdf\/2023\/72_S381.pdf"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,12,17]]},"references-count":23,"URL":"https:\/\/doi.org\/10.33549\/physiolres.935230","relation":{},"ISSN":["1802-9973","0862-8408"],"issn-type":[{"value":"1802-9973","type":"electronic"},{"value":"0862-8408","type":"print"}],"subject":[],"published":{"date-parts":[[2023,12,17]]}}}