{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T06:34:39Z","timestamp":1772001279814,"version":"3.50.1"},"reference-count":29,"publisher":"Institute of Physiology of the Czech Academy of Sciences","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Physiol Res"],"published-print":{"date-parts":[[2024,8,31]]},"abstract":"<jats:p>The effects of alpha-pinene (AP), a monoterpenoid, known for its antioxidant, anti-inflammatory, and anti-apoptotic properties, on methotrexate (MTX)-induced cardiac and hepatic damage were investigated in this study. Male Sprague-Dawley rats were divided into Control, Vehicle, AP, MTX, and AP+MTX groups (n=7). AP (50 mg\/kg\/day, 14 days) was applied subcutaneously in the AP and AP+MTX groups. MTX (20 mg\/kg) was injected three days before sacrification. Serum CK-MB, troponin T, ALT, and AST levels, as well as cardiac and hepatic MDA, GSH, caspase-3, and p53 levels, were measured by ELISA. Histological changes in tissues were evaluated by scoring in terms of tissue damage and cellular degeneration parameters after hematoxylin-eosin staining. MTX caused significant increase in serum CK-MB, troponin T, ALT, and AST levels, hepatic and cardiac lipid peroxidation, GSH depletion, and caspase-3 level. However, tissue levels of p53 did not change significantly. MTX-induced histological deterioration was observed in both tissues. These MTX-induced changes were significantly reduced in the AP+MTX group. Present results show that MTX-induced cardiac and hepatic damage is prevented by AP pretreatment. This protection can be attributed to the antioxidant and anti-apoptotic properties of AP. Considering the importance of MTX in cancer treatment, AP appears to have highly promising potential as a cardioprotective and hepatoprotective agent in anti-tumoral therapy.<\/jats:p>","DOI":"10.33549\/physiolres.935338","type":"journal-article","created":{"date-parts":[[2024,9,9]],"date-time":"2024-09-09T11:51:13Z","timestamp":1725882673000},"page":"621-631","source":"Crossref","is-referenced-by-count":8,"title":["Can Alpha-Pinene Prevent Methotrexate-Induced Cardiac and Hepatic Damage?"],"prefix":"10.33549","author":[{"given":"S","family":"Cirrik","sequence":"first","affiliation":[{"name":"Ordu University, Medical Faculty, Physiology Department, Ordu, Turkiye. selmacrrk@yahoo.com"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G","family":"Hacioglu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E","family":"Kabartan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B","family":"Tezcan Yavuz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Sirin Tomruk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"19169","reference":[{"key":"ref0","doi-asserted-by":"publisher","unstructured":"Bedoui Y, Guillot X, S\u00e9lambarom J, Guiraud P, Giry C, Jaffar-Bandjee MC, Ralandison S, Gasque P. 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