{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T19:36:23Z","timestamp":1791401783867,"version":"4.3.3"},"reference-count":41,"publisher":"Institute of Physiology of the Czech Academy of Sciences","issue":"Suppl 2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Physiol Res"],"abstract":"<jats:p>Short-term fructose exposure may perturb the nitric oxide (NO)\/reactive oxygen species (ROS) balance before hemodynamic changes development. Male Wistar Kyoto (WKY) and spontaneously hypertensive rats (SHR) rats received 10 % fructose in drinking water for 3 weeks or remained on tap water. We assessed systolic blood pressure (tail-cuff), plasma lipid levels, tissue conjugated diene concentrations, protein expression of NADPH oxidase, NF-\u03baB, and SOD (Western blot), and total NO synthase (NOS) activity ([3H]-L-arginine to [3H]-L-citrulline). Fructose did not change blood pressure in either strain, but increased kidney-to-body-weight ratio in SHR. In WKY, plasma HDL level decreased; in SHR, total cholesterol, VLDL, and triglycerides increased. Conjugated diene concentration increased in the kidney of WKY but not in the heart. Fructose upregulated renal NADPH oxidase and downregulated renal SOD in SHR, with no change in cardiac NADPH oxidase. NF-\u03baB protein expression did not change in either tissue. NOS activity decreased in the heart and kidney of WKY and in the kidney of SHR. We can conclude that even moderate, short-term fructose intake induces strain-dependent dyslipidemia and an early shift of the renal redox milieu toward oxidative stress, accompanied by reduced NOS activity, while leaving blood pressure unchanged. The kidney appears more susceptible than the heart, particularly in the hypertensive background, highlighting the NO\/ROS axis as an early target for intervention.<\/jats:p>","DOI":"10.33549\/physiolres.935724","type":"journal-article","created":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T14:32:18Z","timestamp":1768228338000},"page":"S195","source":"Crossref","is-referenced-by-count":1,"title":["Cardio-Renal Effects of Short-Term Fructose Treatment in Hypertensive Rats: Focused on NO\/ROS Balance"],"prefix":"10.33549","volume":"74","author":[{"given":"O","family":"Pechanova","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05ra1x588","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute of Normal and Pathological Physiology of the Slovak Academy of Sciences"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"Bulkova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Vrankova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Klimentova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z","family":"Galandakova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Cebova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"19169","published-online":{"date-parts":[[2025,12,31]]},"reference":[{"key":"ref0","doi-asserted-by":"crossref","unstructured":"1. De Koning L, Malik VS, Kellogg MD, Rimm EB, Willett WC, Hu FB. Sweetened beverage consumption, incident coronary heart disease, and biomarkers of risk in men. Circulation 2012;125:1735-1741. https:\/\/doi.org\/10.1161\/CIRCULATIONAHA.111.067017 DOI: 10.1161\/CIRCULATIONAHA.111.067017","DOI":"10.1161\/CIRCULATIONAHA.111.067017"},{"key":"ref1","doi-asserted-by":"crossref","unstructured":"2. Li B, Yan N, Jiang H, Cui M, Wu M, Wang L, Mi B, Li Z, Shi J, Fan Y, Azalati MM, Li C, Chen F, Ma M, Wang D, Ma L. Consumption of sugar sweetened beverages, artificially sweetened beverages and fruit juices and risk of type 2 diabetes, hypertension, cardiovascular disease, and mortality: A meta-analysis. Front Nutr 2023;10:1019534. https:\/\/doi.org\/10.3389\/fnut.2023.1019534 DOI: 10.3389\/fnut.2023.1019534","DOI":"10.3389\/fnut.2023.1019534"},{"key":"ref2","doi-asserted-by":"crossref","unstructured":"3. Bray GA. Fructose and risk of cardiometabolic disease. Curr Atheroscler Rep 2012;14:570-578. https:\/\/doi.org\/10.1007\/s11883-012-0276-6 DOI: 10.1007\/s11883-012-0276-6","DOI":"10.1007\/s11883-012-0276-6"},{"key":"ref3","doi-asserted-by":"crossref","unstructured":"4. Koepsell H. Glucose transporters in the small intestine in health and disease. Pflugers Arch 2020;472:1207-1248. https:\/\/doi.org\/10.1007\/s00424-020-02439-5, https:\/\/doi.org\/10.1007\/s00424-020-02441-x, https:\/\/doi.org\/10.1007\/ s00424-020-02442-w DOI: 10.1007\/s00424-020-02439-5","DOI":"10.1007\/s00424-020-02439-5"},{"key":"ref4","doi-asserted-by":"crossref","unstructured":"5. Jang C, Wada S, Yang S, Gosis B, Zeng X, Zhang Z, Shen Y, Lee G, Arany Z, Rabinowitz JD. The small intestine shields the liver from fructose-induced steatosis. Nat Metab 2020;2:586-593. https:\/\/doi.org\/10.1038\/s42255-020-0222-9 DOI: 10.1038\/s42255-020-0222-9","DOI":"10.1038\/s42255-020-0222-9"},{"key":"ref5","doi-asserted-by":"crossref","unstructured":"6. Geidl-Flueck B, Gerber PA. Fructose drives de novo lipogenesis affecting metabolic health. J Endocrinol 2023;257:e220270. https:\/\/doi.org\/10.1530\/JOE-22-0270 DOI: 10.1530\/JOE-22-0270","DOI":"10.1530\/JOE-22-0270"},{"key":"ref6","unstructured":"7. Vieira FO, Leal VDE O, Stockler-Pinto MB, Barros ADE F, Borges NA, Lobo JC, Mafra D. Fructose intake: Is there an association with uric acid levels in nondialysis-dependent chronic kidney disease patients? Nutr Hosp 2014;31:772-777. https:\/\/doi.org\/10.3305\/nh.2015.31.2.7796"},{"key":"ref7","doi-asserted-by":"crossref","unstructured":"8. Lubawy M, Formanowicz D. High-fructose diet-induced hyperuricemia accompanying metabolic syndrome-mechanisms and dietary therapy proposals. Int J Environ Res Public Health 2023;20:3596. https:\/\/doi.org\/10.3390\/ijerph20043596 DOI: 10.3390\/ijerph20043596","DOI":"10.3390\/ijerph20043596"},{"key":"ref8","doi-asserted-by":"crossref","unstructured":"9. Gersch C, Palii SP, Kim KM, Angerhofer A, Johnson RJ, Henderson GN. Inactivation of nitric oxide by uric acid. Nucleosides Nucleotides Nucleic Acids 2008;27:967-978. https:\/\/doi.org\/10.1080\/15257770802257952 DOI: 10.1080\/15257770802257952","DOI":"10.1080\/15257770802257952"},{"key":"ref9","doi-asserted-by":"crossref","unstructured":"10. Warren BE, Lou PH, Lucchinetti E, Zhang L, Clanachan AS, Affolter A, Hersberger M, Zaugg M, Lemieux H. Early mitochondrial dysfunction in glycolytic muscle, but not oxidative muscle, of the fructose-fed insulin-resistant rat. Am J Physiol Endocrinol Metab 2014;306(6):E658-67. Erratum in: Am J Physiol Endocrinol Metab 2014;307:E1166. https:\/\/doi.org\/10.1152\/ajpendo.00511.2013 DOI: 10.1152\/ajpendo.00511.2013","DOI":"10.1152\/ajpendo.00511.2013"},{"key":"ref10","doi-asserted-by":"crossref","unstructured":"11. Crescenzo R, Bianco F, Coppola P, Mazzoli A, Cigliano L, Liverini G, Iossa S. Increased skeletal muscle mitochondrial efficiency in rats with fructose-induced alteration in glucose tolerance. Br J Nutr 2013;110:1996-2003. https:\/\/doi.org\/10.1017\/S0007114513001566 DOI: 10.1017\/S0007114513001566","DOI":"10.1017\/S0007114513001566"},{"key":"ref11","doi-asserted-by":"crossref","unstructured":"12. Giacchetti G, Sechi LA, Griffin CA, Don BR, Mantero F, Schambelan M. The tissue renin-angiotensin system in rats with fructose-induced hypertension: overexpression of type 1 angiotensin II receptor in adipose tissue. J Hypertens 2000;18:695-702. https:\/\/doi.org\/10.1097\/00004872-200018060-00006 DOI: 10.1097\/00004872-200018060-00006","DOI":"10.1097\/00004872-200018060-00006"},{"key":"ref12","doi-asserted-by":"crossref","unstructured":"13. Griendling KK, Minieri CA, Ollerenshaw JD, Alexander RW. Angiotensin II stimulates NADH and NADPH oxidase activity in cultured vascular smooth muscle cells. Circ Res 1994; 74:1141-1148. https:\/\/doi.org\/10.1161\/01.RES.74.6.1141 DOI: 10.1161\/01.RES.74.6.1141","DOI":"10.1161\/01.RES.74.6.1141"},{"key":"ref13","doi-asserted-by":"crossref","unstructured":"14. Sachse A, Wolf G. Angiotensin II-induced reactive oxygen species and the kidney. J Am Soc Nephrol 2007;18:2439-2446. https:\/\/doi.org\/10.1681\/ASN.2007020149 DOI: 10.1681\/ASN.2007020149","DOI":"10.1681\/ASN.2007020149"},{"key":"ref14","doi-asserted-by":"crossref","unstructured":"15. Liu H, Colavitti R, Rovira II, Finkel T. Redox-dependent transcriptional regulation. Circ Res 2005;97:967-974. https:\/\/doi.org\/10.1161\/01.RES.0000188210.72062.10 DOI: 10.1161\/01.RES.0000188210.72062.10","DOI":"10.1161\/01.RES.0000188210.72062.10"},{"key":"ref15","doi-asserted-by":"crossref","unstructured":"16. Morgan MJ, Liu ZG. Crosstalk of reactive oxygen species and NF-kB Signaling. Cell Res 2011;21:103-115. https:\/\/doi.org\/10.1038\/cr.2010.178 DOI: 10.1038\/cr.2010.178","DOI":"10.1038\/cr.2010.178"},{"key":"ref16","doi-asserted-by":"crossref","unstructured":"17. Quilley J (2011). Oxidative Stress and inflammation in the endothelial dysfunction of obesity: A role for NFkB? J Hypertens 2010;28:2010-2011. https:\/\/doi.org\/10.1097\/HJH.0b013e32833e24cb DOI: 10.1097\/HJH.0b013e32833e24cb","DOI":"10.1097\/HJH.0b013e32833e24cb"},{"key":"ref17","doi-asserted-by":"crossref","unstructured":"18. Nakagawa T, Johnson RJ, Andres-Hernando A, Roncal-Jimenez C, Sanchez-Lozada LG, Tolan DR, Lanaspa MA. Fructose production and metabolism in the kidney. J Am Soc Nephrol 2020;31:898-906. https:\/\/doi.org\/10.1681\/ASN.2019101015 DOI: 10.1681\/ASN.2019101015","DOI":"10.1681\/ASN.2019101015"},{"key":"ref18","doi-asserted-by":"crossref","unstructured":"19. Scurt FG, Ganz MJ, Herzog C, Bose K, Mertens PR, Chatzikyrkou C. Association of metabolic syndrome and chronic kidney disease. Obes Rev 2024;25(1):e13649. https:\/\/doi.org\/10.1111\/obr.13649 DOI: 10.1111\/obr.13649","DOI":"10.1111\/obr.13649"},{"key":"ref19","doi-asserted-by":"crossref","unstructured":"20. Cebova M, Klimentova J, Janega P, Pechanova O. Effect of bioactive compound of Aronia melanocarpa on cardiovascular system in experimental hypertension. Oxid Med Cell Longev 2017;2017:8156594. https:\/\/doi.org\/10.1155\/2017\/8156594 DOI: 10.1155\/2017\/8156594","DOI":"10.1155\/2017\/8156594"},{"key":"ref20","doi-asserted-by":"crossref","unstructured":"21. Pechanova O, Barta A, Koneracka M, Zavisova V, Kubovcikova M, Klimentova J, T\u04e7r\u04e7k J, Zemancikova A, Cebova M. Protective effects of nanoparticle-loaded aliskiren on cardiovascular system in spontaneously hypertensive rats. Molecules 2019;24:2710. https:\/\/doi.org\/10.3390\/molecules24152710 DOI: 10.3390\/molecules24152710","DOI":"10.3390\/molecules24152710"},{"key":"ref21","doi-asserted-by":"crossref","unstructured":"22. \u015eaman E, Cebova M, Barta A, Koneracka M, Zavisova V, Eckstein-Andicsova A, Danko M, Mosnacek J, Pechanova O. Combined therapy with simvastatin- and Coenzyme-Q10-loaded nanoparticles upregulates the Akt-eNOS pathway in experimental metabolic syndrome. Int J Mol Sci 2022; 24:276. https:\/\/doi.org\/10.3390\/ijms24010276 DOI: 10.3390\/ijms24010276","DOI":"10.3390\/ijms24010276"},{"key":"ref22","doi-asserted-by":"crossref","unstructured":"23. La Russa D, Brunelli E, Pellegrino D. Oxidative imbalance and kidney damage in spontaneously hypertensive rats: activation of extrinsic apoptotic pathways. Clin Sci (Lond) 2017;131:1419-1428. https:\/\/doi.org\/10.1042\/CS20170177 DOI: 10.1042\/CS20170177","DOI":"10.1042\/CS20170177"},{"key":"ref23","doi-asserted-by":"crossref","unstructured":"24. Dovinova I, Kvandova M, Balis P, Gresova L, Majzunova M, Horakova L, Chan JY, Barancik M. The role of Nrf2 and PPARgamma in the improvement of oxidative stress in hypertension and cardiovascular diseases. Physiol Res 2020;69(Suppl 4):S541-S553. https:\/\/doi.org\/10.33549\/physiolres.934612 DOI: 10.33549\/physiolres.934612","DOI":"10.33549\/physiolres.934612"},{"key":"ref24","doi-asserted-by":"crossref","unstructured":"25. Vokurkov\u00e1 M, Rauchov\u00e1 H, \u0158ez\u00e1\u010dov\u00e1 L, Van\u011b\u010dkov\u00e1 I, Zicha J. ROS production is increased in the kidney but not in the brain of Dahl rats with salt hypertension elicited in adulthood. Physiol Res 2015;64:303-312. https:\/\/doi.org\/10.33549\/physiolres.933054 DOI: 10.33549\/physiolres.933054","DOI":"10.33549\/physiolres.933054"},{"key":"ref25","doi-asserted-by":"crossref","unstructured":"26. Koj\u0161ov\u00e1 S, Jendekov\u00e1 L, Zicha J, Kune\u0161 J, Andriantsitohaina R, Pech\u00e1\u0148ov\u00e1 O: the effect of different antioxidants on nitric oxide production in hypertensive rats. Physiol Res 2006;55 (Suppl.1):S3-S16. https:\/\/doi.org\/10.33549\/physiolres.930000.55.S1.3 DOI: 10.33549\/physiolres.930000.55.S1.3","DOI":"10.33549\/physiolres.930000.55.S1.3"},{"key":"ref26","doi-asserted-by":"crossref","unstructured":"27. D'Angelo G, Elmarakby AA, Pollock DM, Stepp DW. Fructose feeding increases insulin resistance but not blood pressure in Sprague-Dawley rats. Hypertension 2005;46:806-811. https:\/\/doi.org\/10.1161\/ 01.HYP.0000182697.39687.34 DOI: 10.1161\/01.HYP.0000182697.39687.34","DOI":"10.1161\/01.HYP.0000182697.39687.34"},{"key":"ref27","doi-asserted-by":"crossref","unstructured":"28. S\u00e1nchez-Lozada LG, Tapia E, Jim\u00e9nez A, Bautista P, Crist\u00f3bal M, Nepomuceno T. Fructose-induced metabolic syndrome is associated with glomerular hypertension and renal microvascular damage in rats. Am J Physiol Renal Physiol 2007;292:F423-F429. https:\/\/doi.org\/10.1152\/ajprenal.00124.2006 DOI: 10.1152\/ajprenal.00124.2006","DOI":"10.1152\/ajprenal.00124.2006"},{"key":"ref28","doi-asserted-by":"crossref","unstructured":"29. Zeman\u010d\u00edkov\u00e1 A, T\u00f6r\u00f6k J. Effect of perivascular adipose tissue on arterial adrenergic contractions in normotensive and hypertensive rats with high fructose intake. Physiol Res 2017;66(Suppl 4):S537-S544. https:\/\/doi.org\/10.33549\/physiolres.933798 DOI: 10.33549\/physiolres.933798","DOI":"10.33549\/physiolres.933798"},{"key":"ref29","doi-asserted-by":"crossref","unstructured":"30. Johnson RJ, Sanchez-Lozada LG, Nakagawa T. The effect of fructose on renal biology and disease. J Am Soc Nephrol 2010;21:2036-2039. https:\/\/doi.org\/10.1681\/ASN.2010050506 DOI: 10.1681\/ASN.2010050506","DOI":"10.1681\/ASN.2010050506"},{"key":"ref30","doi-asserted-by":"crossref","unstructured":"31. Nakayama T, Kosugi T, Gersch M, Connor T, Sanchez-Lozada LG, Lanaspa MA, Roncal C, Perez-Pozo SE, Johnson RJ, Nakagawa T. Dietary fructose causes tubulointerstitial injury in the normal rat kidney. Am J Physiol Renal Physiol 2010;298:F712-F720. https:\/\/doi.org\/10.1152\/ajprenal.00433.2009 DOI: 10.1152\/ajprenal.00433.2009","DOI":"10.1152\/ajprenal.00433.2009"},{"key":"ref31","doi-asserted-by":"crossref","unstructured":"32. Stanhope KL, Schwarz JM, Keim NL, Griffen SC, Bremer AA, Graham JL, Hatcher B, et al. Consuming fructose-sweetened, not glucose-sweetened, beverages increase visceral adiposity and lipids and decreases insulin sensitivity in overweight\/obese humans. J Clin Invest 2009;119:1322-1334. https:\/\/doi.org\/10.1172\/JCI37385 DOI: 10.1172\/JCI37385","DOI":"10.1172\/JCI37385"},{"key":"ref32","doi-asserted-by":"crossref","unstructured":"33. Mikul\u00edkov\u00e1 K, Eckhardt A, Kunes J, Zicha J, Mik\u0161\u00edk I. Advanced glycation end-product pentosidine accumulates in various tissues of rats with high fructose intake. Physiol Res 2008;57:89-94. https:\/\/doi.org\/10.33549\/physiolres.931093 DOI: 10.33549\/physiolres.931093","DOI":"10.33549\/physiolres.931093"},{"key":"ref33","doi-asserted-by":"crossref","unstructured":"34. Geidl-Flueck B, Hochuli M, N\u00e9meth \u00c1, Eberl A, Derron N, K\u00f6feler HC, Tappy L, et al. Fructose- and sucrose- but not glucose-sweetened beverages promote hepatic de novo lipogenesis: A randomized controlled trial. J Hepatol 2021;75:46-54. https:\/\/doi.org\/10.1016\/j.jhep.2021.02.027 DOI: 10.1016\/j.jhep.2021.02.027","DOI":"10.1016\/j.jhep.2021.02.027"},{"key":"ref34","doi-asserted-by":"crossref","unstructured":"35. Ichigo Y, Takeshita A, Hibino M, Nakagawa T, Hayakawa T, Patel D, Field CJ, Shimada M. High-fructose diet-induced hypertriglyceridemia is associated with enhanced hepatic expression of ACAT2 in rats. Physiol Res 2019;68:1021-1026. https:\/\/doi.org\/10.33549\/physiolres.934226 DOI: 10.33549\/physiolres.934226","DOI":"10.33549\/physiolres.934226"},{"key":"ref35","doi-asserted-by":"crossref","unstructured":"36. Yang Q, Wu FR, Wang JN, Gao L, Jiang L, Li HD, Ma Q, et al. Nox4 in renal diseases: An update. Free Radic Biol Med 2018;124:466-472. https:\/\/doi.org\/10.1016\/j.freeradbiomed.2018.06.042 DOI: 10.1016\/j.freeradbiomed.2018.06.042","DOI":"10.1016\/j.freeradbiomed.2018.06.042"},{"key":"ref36","doi-asserted-by":"crossref","unstructured":"37. Shi X, Qiu H. New insights into energy substrate utilization and metabolic remodeling in cardiac physiological adaption. Front Physiol 2022;13:831829. https:\/\/doi.org\/10.3389\/fphys.2022.831829 DOI: 10.3389\/fphys.2022.831829","DOI":"10.3389\/fphys.2022.831829"},{"key":"ref37","doi-asserted-by":"crossref","unstructured":"38. Sasv\u00e1riov\u00e1 M, Salvaras L, Se\u010dk\u00e1rov\u00e1 Mich\u00e1likov\u00e1 D, Tyukos Kaprinay B, Knezl V, G\u00e1sp\u00e1rov\u00e1 Z, Stankovi\u010dov\u00e1 T. Assessment of the cardiovascular risk of high-fat-high-fructose diet in hereditary hypertriacylglycerolemic rats and venlafaxine effect. Physiol Res 2024;73:973-984. https:\/\/doi.org\/10.33549\/physiolres.935420 DOI: 10.33549\/physiolres.935420","DOI":"10.33549\/physiolres.935420"},{"key":"ref38","doi-asserted-by":"crossref","unstructured":"39. Miatello R, Risler N, Castro C, Gonz\u00e1lez S, R\u00fcttler M, Cruzado M. Aortic smooth muscle cell proliferation and endothelial nitric oxide synthase activity in fructose-fed rats. Am J Hypertens 2001;14:1135-1141. https:\/\/doi.org\/10.1016\/S0895-7061(01)02206-3 DOI: 10.1016\/S0895-7061(01)02206-3","DOI":"10.1016\/S0895-7061(01)02206-3"},{"key":"ref39","doi-asserted-by":"crossref","unstructured":"40. Palanisamy N, Venkataraman AC. beneficial effect of genistein on lowering blood pressure and kidney toxicity in fructose-fed hypertensive rats. Br J Nutr 2013;109:1806-1812. https:\/\/doi.org\/10.1017\/S0007114512003819 DOI: 10.1017\/S0007114512003819","DOI":"10.1017\/S0007114512003819"},{"key":"ref40","doi-asserted-by":"crossref","unstructured":"41. Okamura T, Tawa M, Geddawy A, Shimosato T, Iwasaki H, Shintaku H, Yoshida Y, Masada M, Shinozaki K, Imamura T. Effects of atorvastatin, amlodipine, and their combination on vascular dysfunction in insulin-resistant rats. J Pharmacol Sci 2014;124:76-85. https:\/\/doi.org\/10.1254\/jphs.13178FP DOI: 10.1254\/jphs.13178FP","DOI":"10.1254\/jphs.13178FP"}],"container-title":["Physiological Research"],"original-title":[],"deposited":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T20:18:30Z","timestamp":1768249110000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.biomed.cas.cz\/physiolres\/pdf\/2025\/74_S195.pdf"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,31]]},"references-count":41,"journal-issue":{"issue":"Suppl 2","published-online":{"date-parts":[[2025,12,31]]}},"URL":"https:\/\/doi.org\/10.33549\/physiolres.935724","relation":{},"ISSN":["0862-8408","1802-9973"],"issn-type":[{"value":"0862-8408","type":"print"},{"value":"1802-9973","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,31]]}}}