{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T12:03:54Z","timestamp":1784289834271,"version":"3.55.0"},"reference-count":24,"publisher":"Institute of Physiology of the Czech Academy of Sciences","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Adenomyosis is a chronic gynecological disorder characterized by ectopic endometrial tissue within the myometrium, leading to pelvic pain, menorrhagia, and infertility. Increasing evidence implicates dysregulated Wnt\/\u03b2-catenin signaling and aberrant activation of SFRP4+ natural killer T (NKT) cells in disease progression. This study reports the ultrasound-assisted synthesis of a cerium-doped zeolite nanocomplex (Ce-ZNC) and evaluates its therapeutic efficacy in experimental models of adenomyosis. Ce-ZNC was synthesized via an ultrasound-assisted doping approach and characterized using transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), and polydispersity index (PDI) analysis. In vitro studies were conducted on human peripheral blood mononuclear cell (PBMC)-derived SFRP4\u207a NKT cells. In vivo efficacy was evaluated in a murine adenomyosis model. IGFBP5 expression and Wnt\/\u03b2 catenin signaling components were analyzed by RT-qPCR and Western blotting. Histological and cytokine analyses were performed to assess fibrosis and inflammation. Ce-ZNC exhibited spherical morphology with nanoscale dimensions and good colloidal stability. Treatment resulted in a dose-dependent suppression of IGFBP5 expression and inhibition of Wnt\/\u03b2-catenin signaling. In vivo administration significantly reduced uterine fibrosis, glandular invasion, and inflammatory cytokine levels. These findings suggest that Ce-ZNC exerts immunomodulatory and anti-fibrotic effects in adenomyosis. While promising, further studies are required to elucidate biodistribution, long-term safety, and translational potential in human models.<\/jats:p>","DOI":"10.33549\/physiolres.935738","type":"journal-article","created":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T11:14:39Z","timestamp":1784286879000},"page":"499","source":"Crossref","is-referenced-by-count":0,"title":["Ultrasound-Assisted Cerium-Doped Zeolite Nanocomplex Attenuates Adenomyosis via Wnt\/\u03b2-Catenin Pathway Inhibition and IGFBP5 Downregulation in SFRP4\u207a NKT Cells"],"prefix":"10.33549","author":[{"given":"X","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"19169","published-online":{"date-parts":[[2026,7,7]]},"reference":[{"key":"ref0","doi-asserted-by":"crossref","unstructured":"1.\tDi Donato N, Montanari G, Benfenati A, Leonardi D, Bertoldo V, Monti G, Raimondo D, Seracchioli R. 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