{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T18:07:02Z","timestamp":1783706822568,"version":"3.55.0"},"reference-count":116,"publisher":"Institute of Physiology of the Czech Academy of Sciences","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>The role of M4 muscarinic receptors in locomotor activity regulation remains controversial, with conflicting findings hampering our understanding of movement disorders. This uncertainty is further complicated by the unexplored relationship between M4 receptors and biological rhythms, as well as potential sex-specific effects that may explain previous inconsistent results. Through systematic investigation of locomotor functions in biological rhythm paradigms, we discovered that M4 muscarinic receptors significantly modulate locomotor activity rhythms via brain pacemaker mechanisms. Notably, this regulatory effect displayed marked sexual dimorphism, being present exclusively in females, suggesting crucial interactions with sex hormones. Our research identified the striatum as the key structure directing these locomotor biological rhythms. These findings provide critical insights into the sex-specific nature of M4 receptor function and establish a new framework for understanding hyperactivity disorders, particularly those exhibiting sex-based differences in prevalence or manifestation.<\/jats:p>","DOI":"10.33549\/physiolres.935750","type":"journal-article","created":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T13:06:10Z","timestamp":1768309570000},"page":"S1","source":"Crossref","is-referenced-by-count":1,"title":["The Novel Functions of M4 Muscarinic Receptors"],"prefix":"10.33549","author":[{"given":"J","family":"Myslivecek","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K","family":"Janisova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"19169","published-online":{"date-parts":[[2025,12,31]]},"reference":[{"key":"ref0","doi-asserted-by":"crossref","unstructured":"1.\tMyslivecek J. Multitargeting nature of muscarinic orthosteric agonists and antagonists. Front Physiol 2022;13:974160. https:\/\/doi.org\/10.3389\/fphys.2022.974160","DOI":"10.3389\/fphys.2022.974160"},{"key":"ref1","doi-asserted-by":"crossref","unstructured":"2.\tAmenta F, Tayebati SK. Pathways of acetylcholine synthesis, transport and release as targets for treatment of adult-onset cognitive dysfunction. Curr Med Chem 2008;15:488-498. https:\/\/doi.org\/10.2174\/092986708783503203","DOI":"10.2174\/092986708783503203"},{"key":"ref2","doi-asserted-by":"crossref","unstructured":"3.\tNguyen ML, Cox GD, Parsons SM. Kinetic parameters for the vesicular acetylcholine transporter: two protons are exchanged for one acetylcholine. Biochemistry 1998;37:13400-13410. https:\/\/doi.org\/10.1021\/bi9802263","DOI":"10.1021\/bi9802263"},{"key":"ref3","doi-asserted-by":"crossref","unstructured":"4.\tPerrier AL, Massoulie J, Krejci E. PRiMA: the membrane anchor of acetylcholinesterase in the brain. Neuron 2002;33:275-285. https:\/\/doi.org\/10.1016\/S0896-6273(01)00584-0","DOI":"10.1016\/S0896-6273(01)00584-0"},{"key":"ref4","doi-asserted-by":"crossref","unstructured":"5.\tDobbertin A, Hrabovska A, Dembele K, Camp S, Taylor P, Krejci E, Bernard V. Targeting of Acetylcholinesterase in Neurons In Vivo: A Dual Processing Function for the Proline-Rich Membrane Anchor Subunit and the Attachment Domain on the Catalytic Subunit. J Neurosci 2009;29:4519-4530. https:\/\/doi.org\/10.1523\/JNEUROSCI.3863-08.2009","DOI":"10.1523\/JNEUROSCI.3863-08.2009"},{"key":"ref5","doi-asserted-by":"crossref","unstructured":"6.\tFarar V, Mohr F, Legrand M, Lamotte d'Incamps B, Cendelin J, Leroy J, Abitbol M, et al. Near-complete adaptation of the PRiMA knockout to the lack of central acetylcholinesterase. J Neurochem 2012;122:1065-1080. https:\/\/doi.org\/10.1111\/j.1471-4159.2012.07856.x","DOI":"10.1111\/j.1471-4159.2012.07856.x"},{"key":"ref6","doi-asserted-by":"crossref","unstructured":"7.\tEglen R. Overview of muscarinic receptor subtypes. In: Muscarinic Receptors. Fryer AD, Christopoulos A, Nathanson NM (eds), Springer Berlin, Heidelberg, 2012, pp 3-28. https:\/\/doi.org\/10.1007\/978-3-642-23274-9_1","DOI":"10.1007\/978-3-642-23274-9_1"},{"key":"ref7","doi-asserted-by":"crossref","unstructured":"8.\tCampoy FJ, Vidal CJ, Mu\u00f1oz-Delgado E, Montenegro MF, Cabezas-Herrera J, Nieto-Cer\u00f3n S. Cholinergic system and cell proliferation. Chem Biol Interact 2016;259:257-265. https:\/\/doi.org\/10.1016\/j.cbi.2016.04.014","DOI":"10.1016\/j.cbi.2016.04.014"},{"key":"ref8","doi-asserted-by":"crossref","unstructured":"9.\tCroy CH, Schober DA, Xiao H, Quets A, Christopoulos A, Felder CC. Characterization of the novel positive allosteric modulator, LY2119620, at the muscarinic M(2) and M(4) receptors. Mol Pharmacol 2014;86:106-115. https:\/\/doi.org\/10.1124\/mol.114.091751","DOI":"10.1124\/mol.114.091751"},{"key":"ref9","doi-asserted-by":"crossref","unstructured":"10.\tCaulfield MP. Muscarinic receptors--characterization, coupling and function. Pharmacol Ther 1993;58:319-379. https:\/\/doi.org\/10.1016\/0163-7258(93)90027-B","DOI":"10.1016\/0163-7258(93)90027-B"},{"key":"ref10","doi-asserted-by":"crossref","unstructured":"11.\tWootten D, Christopoulos A, Marti-Solano M, Babu MM, Sexton PM. Mechanisms of signalling and biased agonism in G protein-coupled receptors. Nat Rev Mol Cell Biol 2018;19:638-653. https:\/\/doi.org\/10.1038\/s41580-018-0049-3","DOI":"10.1038\/s41580-018-0049-3"},{"key":"ref11","doi-asserted-by":"crossref","unstructured":"12.\tvan der Westhuizen ET, Choy KHC, Valant C, McKenzie-Nickson S, Bradley SJ, Tobin AB, Sexton PM, Christopoulos A. Fine Tuning Muscarinic Acetylcholine Receptor Signaling Through Allostery and Bias. Front Pharmacol 2021;11:606656. https:\/\/doi.org\/10.3389\/fphar.2020.606656","DOI":"10.3389\/fphar.2020.606656"},{"key":"ref12","doi-asserted-by":"crossref","unstructured":"13.\tKruse AC, Ring AM, Manglik A, Hu J, Hu K, Eitel K, Hubner H, et al. Activation and allosteric modulation of a muscarinic acetylcholine receptor. Nature 2013;504:101-106. https:\/\/doi.org\/10.1038\/nature12735","DOI":"10.1038\/nature12735"},{"key":"ref13","doi-asserted-by":"crossref","unstructured":"14.\tRand\u00e1kov\u00e1 A, Jakub\u00edk J. Functionally selective and biased agonists of muscarinic receptors. Pharmacol Res 2021;169:105641. https:\/\/doi.org\/10.1016\/j.phrs.2021.105641","DOI":"10.1016\/j.phrs.2021.105641"},{"key":"ref14","doi-asserted-by":"crossref","unstructured":"15.\tBrady AE, Jones CK, Bridges TM, Kennedy JP, Thompson AD, Heiman JU, Breininger ML, et al. Centrally active allosteric potentiators of the M4 muscarinic acetylcholine receptor reverse amphetamine-induced hyperlocomotor activity in rats. J Pharmacol Exp Ther 2008;327:941-953. https:\/\/doi.org\/10.1124\/jpet.108.140350","DOI":"10.1124\/jpet.108.140350"},{"key":"ref15","doi-asserted-by":"crossref","unstructured":"16.\tCroy CH, Chan WY, Castetter AM, Watt ML, Quets AT, Felder CC. Characterization of PCS1055, a novel muscarinic M4 receptor antagonist. Eur J Pharmacol 2016;782:70-76. https:\/\/doi.org\/10.1016\/j.ejphar.2016.04.022","DOI":"10.1016\/j.ejphar.2016.04.022"},{"key":"ref16","doi-asserted-by":"crossref","unstructured":"17.\tGentry PR, Bridges TM, Lamsal A, Vinson PN, Smith E, Chase P, Hodder PS, et al. Discovery of ML326: The first sub-micromolar, selective M5 PAM. Bioorg Med Chem Lett 2013;23:2996-3000. https:\/\/doi.org\/10.1016\/j.bmcl.2013.03.032","DOI":"10.1016\/j.bmcl.2013.03.032"},{"key":"ref17","doi-asserted-by":"crossref","unstructured":"18.\tGentry PR, Kokubo M, Bridges TM, Cho HP, Smith E, Chase P, Hodder PS, et al. Discovery, synthesis and characterization of a highly muscarinic acetylcholine receptor (mAChR)-selective M5-orthosteric antagonist, VU0488130 (ML381): a novel molecular probe. ChemMedChem 2014;9:1677-1682. https:\/\/doi.org\/10.1002\/cmdc.201402051","DOI":"10.1002\/cmdc.201402051"},{"key":"ref18","doi-asserted-by":"crossref","unstructured":"19.\tMa L, Seager MA, Wittmann M, Jacobson M, Bickel D, Burno M, Jones K, et al. Selective activation of the M1 muscarinic acetylcholine receptor achieved by allosteric potentiation. Proc Natl Acad Sci U S A 2009;106:15950-15955. https:\/\/doi.org\/10.1073\/pnas.0900903106","DOI":"10.1073\/pnas.0900903106"},{"key":"ref19","doi-asserted-by":"crossref","unstructured":"20.\tSheffler DJ, Williams R, Bridges TM, Xiang Z, Kane AS, Byun NE, Jadhav S, et al. A Novel Selective Muscarinic Acetylcholine Receptor Subtype 1 Antagonist Reduces Seizures without Impairing Hippocampus-Dependent Learning. Mol Pharmacol 2009;76:356-368. https:\/\/doi.org\/10.1124\/mol.109.056531","DOI":"10.1124\/mol.109.056531"},{"key":"ref20","doi-asserted-by":"crossref","unstructured":"21.\tSteinfeld T, Mammen M, Smith JA, Wilson RD, Jasper JR. A novel multivalent ligand that bridges the allosteric and orthosteric binding sites of the M2 muscarinic receptor. Mol Pharmacol 2007;72:291-302. https:\/\/doi.org\/10.1124\/mol.106.033746","DOI":"10.1124\/mol.106.033746"},{"key":"ref21","doi-asserted-by":"crossref","unstructured":"22.\tTr\u00e4nkle C, Dittmann A, Schulz U, Weyand O, Buller S, J\u00f6hren K, Heller E, Birdsall NJ, Holzgrabe U, Ellis J, H\u00f6ltje HD, Mohr K. Atypical muscarinic allosteric modulation: cooperativity between modulators and their atypical binding topology in muscarinic M2 and M2\/M5 chimeric receptors. Mol Pharmacol 2005;68:1597-1610. https:\/\/doi.org\/10.1124\/mol.105.017707","DOI":"10.1124\/mol.105.017707"},{"key":"ref22","doi-asserted-by":"crossref","unstructured":"23.\tAnagnostaras SG, Maren S, Fanselow MS. Scopolamine selectively disrupts the acquisition of contextual fear conditioning in rats. Neurobiol Learn Mem 1995;64:191-194. https:\/\/doi.org\/10.1006\/nlme.1995.0001","DOI":"10.1006\/nlme.1995.0001"},{"key":"ref23","doi-asserted-by":"crossref","unstructured":"24.\tChen KC, Baxter MG, Rodefer JS. Central blockade of muscarinic cholinergic receptors disrupts affective and attentional set-shifting. Eur J Neurosci 2004;20:1081-1088. https:\/\/doi.org\/10.1111\/j.1460-9568.2004.03548.x","DOI":"10.1111\/j.1460-9568.2004.03548.x"},{"key":"ref24","doi-asserted-by":"crossref","unstructured":"25.\tSipos ML, Burchnell V, Galbicka G. Dose-response curves and time-course effects of selected anticholinergics on locomotor activity in rats. Psychopharmacology 1999;147:250-256. https:\/\/doi.org\/10.1007\/s002130051164","DOI":"10.1007\/s002130051164"},{"key":"ref25","doi-asserted-by":"crossref","unstructured":"26.\tSanford LD, Yang L, Tang X, Dong E, Ross RJ, Morrison AR. Cholinergic regulation of the central nucleus of the amygdala in rats: effects of local microinjections of cholinomimetics and cholinergic antagonists on arousal and sleep. Neuroscience 2006;141:2167-2176. https:\/\/doi.org\/10.1016\/j.neuroscience.2006.05.064","DOI":"10.1016\/j.neuroscience.2006.05.064"},{"key":"ref26","doi-asserted-by":"crossref","unstructured":"27.\tPratt WE, Blackstone K. Nucleus accumbens acetylcholine and food intake: decreased muscarinic tone reduces feeding but not food-seeking. Behav Brain Res 2009;198:252-257. https:\/\/doi.org\/10.1016\/j.bbr.2008.11.008","DOI":"10.1016\/j.bbr.2008.11.008"},{"key":"ref27","doi-asserted-by":"crossref","unstructured":"28.\tCrespo JA, St\u00f6ckl P, Zorn K, Saria A, Zernig G. Nucleus accumbens core acetylcholine is preferentially activated during acquisition of drug- vs food-reinforced behavior. Neuropsychopharmacology 2008;33:3213-3220. https:\/\/doi.org\/10.1038\/npp.2008.48","DOI":"10.1038\/npp.2008.48"},{"key":"ref28","doi-asserted-by":"crossref","unstructured":"29.\tGomeza J, Zhang L, Kostenis E, Felder C, Bymaster F, Brodkin J, Shannon H, et al. Enhancement of D1 dopamine receptor-mediated locomotor stimulation in M4 muscarinic acetylcholine receptor knockout mice. Proc Natl Acad Sci U S A 1999;96:10483-10488. https:\/\/doi.org\/10.1073\/pnas.96.18.10483","DOI":"10.1073\/pnas.96.18.10483"},{"key":"ref29","doi-asserted-by":"crossref","unstructured":"30.\tBubser M, Bridges TM, Dencker D, Gould RW, Grannan M, Noetzel MJ, Lamsal A, et al. Selective Activation of M4 Muscarinic Acetylcholine Receptors Reverses MK-801-Induced Behavioral Impairments and Enhances Associative Learning in Rodents. ACS Chem Neurosci 2014;5:920-942. https:\/\/doi.org\/10.1021\/cn500128b","DOI":"10.1021\/cn500128b"},{"key":"ref30","doi-asserted-by":"crossref","unstructured":"31.\tKoshimizu H, Leiter L, Miyakawa T. M4 muscarinic receptor knockout mice display abnormal social behavior and decreased prepulse inhibition. Mol Brain 2012;5:10. https:\/\/doi.org\/10.1186\/1756-6606-5-10","DOI":"10.1186\/1756-6606-5-10"},{"key":"ref31","doi-asserted-by":"crossref","unstructured":"32.\tThomsen M, Lindsley C, Conn PJ, Wessell J, Fulton B, Wess J, Caine SB. Contribution of both M1 and M4 receptors to muscarinic agonist-mediated attenuation of the cocaine discriminative stimulus in mice. Psychopharmacology 2012;220:673-685. https:\/\/doi.org\/10.1007\/s00213-011-2516-9","DOI":"10.1007\/s00213-011-2516-9"},{"key":"ref32","doi-asserted-by":"crossref","unstructured":"33.\tTzavara ET, Bymaster FP, Davis RJ, Wade MR, Perry KW, Wess J, McKinzie DL, et al. M4 muscarinic receptors regulate the dynamics of cholinergic and dopaminergic neurotransmission: relevance to the pathophysiology and treatment of related central nervous system pathologies. FASEB J 2004;18:1410-1412. https:\/\/doi.org\/10.1096\/fj.04-1575fje","DOI":"10.1096\/fj.04-1575fje"},{"key":"ref33","doi-asserted-by":"crossref","unstructured":"34.\tCarr DB, Surmeier DJ. M1 muscarinic receptor modulation of Kir2 channels enhances temporal summation of excitatory synaptic potentials in prefrontal cortex pyramidal neurons. J Neurophysiol 2007;97:3432-3438. https:\/\/doi.org\/10.1152\/jn.00828.2006","DOI":"10.1152\/jn.00828.2006"},{"key":"ref34","doi-asserted-by":"crossref","unstructured":"35.\tBrown D. Muscarinic Acetylcholine Receptors (mAChRs) in the Nervous System: Some Functions and Mechanisms. J Mol Neurosci 2010;41:340-346. https:\/\/doi.org\/10.1007\/s12031-010-9377-2","DOI":"10.1007\/s12031-010-9377-2"},{"key":"ref35","doi-asserted-by":"crossref","unstructured":"36.\tBonsi P, Martella G, Cuomo D, Platania P, Sciamanna G, Bernardi G, Wess J, Pisani A. Loss of Muscarinic Autoreceptor Function Impairs Long-Term Depression But Not Long-Term Potentiation in the Striatum. J Neurosci 2008;28:6258-6263. https:\/\/doi.org\/10.1523\/JNEUROSCI.1678-08.2008","DOI":"10.1523\/JNEUROSCI.1678-08.2008"},{"key":"ref36","doi-asserted-by":"crossref","unstructured":"37.\tMalenka RC, Bear MF. LTP and LTD: an embarrassment of riches. Neuron 2004;44:5-21. https:\/\/doi.org\/10.1016\/j.neuron.2004.09.012","DOI":"10.1016\/j.neuron.2004.09.012"},{"key":"ref37","doi-asserted-by":"crossref","unstructured":"38.\tHersch SM, Gutekunst CA, Rees HD, Heilman CJ, Levey AI. Distribution of m1-m4 muscarinic receptor proteins in the rat striatum: light and electron microscopic immunocytochemistry using subtype-specific antibodies. J Neurosci 1994;14:3351-3363. https:\/\/doi.org\/10.1523\/JNEUROSCI.14-05-03351.1994","DOI":"10.1523\/JNEUROSCI.14-05-03351.1994"},{"key":"ref38","doi-asserted-by":"crossref","unstructured":"39.\tInce E, Ciliax BJ, Levey AI. Differential expression of D1 and D2 dopamine and m4 muscarinic acetylcholine receptor proteins in identified striatonigral neurons. Synapse 1997;27:357-366. https:\/\/doi.org\/10.1002\/(SICI)1098-2396(199712)27:4<357::AID-SYN9>3.0.CO;2-B","DOI":"10.1002\/(SICI)1098-2396(199712)27:4<357::AID-SYN9>3.0.CO;2-B"},{"key":"ref39","doi-asserted-by":"crossref","unstructured":"40.\tMyslivecek J. Two Players in the Field: Hierarchical Model of Interaction between the Dopamine and Acetylcholine Signaling Systems in the Striatum. Biomedicines 2021;9:25. https:\/\/doi.org\/10.3390\/biomedicines9010025","DOI":"10.3390\/biomedicines9010025"},{"key":"ref40","doi-asserted-by":"crossref","unstructured":"41.\tSugaya K, Clamp C, Bryan D, McKinney M. mRNA for the m4 muscarinic receptor subtype is expressed in adult rat brain cholinergic neurons. Brain Res Mol Brain Res 1997;50:305-313. https:\/\/doi.org\/10.1016\/S0169-328X(97)00199-X","DOI":"10.1016\/S0169-328X(97)00199-X"},{"key":"ref41","doi-asserted-by":"crossref","unstructured":"42.\tLevey AI, Kitt CA, Simonds WF, Price DL, Brann MR. Identification and localization of muscarinic acetylcholine receptor proteins in brain with subtype-specific antibodies. J Neurosci 1991;11:3218-3226. https:\/\/doi.org\/10.1523\/JNEUROSCI.11-10-03218.1991","DOI":"10.1523\/JNEUROSCI.11-10-03218.1991"},{"key":"ref42","doi-asserted-by":"crossref","unstructured":"43.\tYasuda RP, Ciesla W, Flores LR, Wall SJ, Li M, Satkus SA, Weisstein JS, et al. Development of antisera selective for m4 and m5 muscarinic cholinergic receptors: distribution of m4 and m5 receptors in rat brain. Mol Pharmacol 1993;43:149-157. https:\/\/doi.org\/10.1016\/S0026-895X(25)13594-3","DOI":"10.1016\/S0026-895X(25)13594-3"},{"key":"ref43","doi-asserted-by":"crossref","unstructured":"44.\tBymaster FP, McKinzie DL, Felder CC, Wess J. Use of M1-M5 muscarinic receptor knockout mice as novel tools to delineate the physiological roles of the muscarinic cholinergic system. Neurochem Res 2003;28:437-442. https:\/\/doi.org\/10.1023\/A:1022844517200","DOI":"10.1023\/A:1022844517200"},{"key":"ref44","doi-asserted-by":"crossref","unstructured":"45.\tCrusio WE, Goldowitz D, Holmes A, Wolfer D. Standards for the publication of mouse mutant studies. Genes Brain Behav 2009;8:1-4. https:\/\/doi.org\/10.1111\/j.1601-183X.2008.00438.x","DOI":"10.1111\/j.1601-183X.2008.00438.x"},{"key":"ref45","doi-asserted-by":"crossref","unstructured":"46.\tBymaster FP, Carter PA, Zhang L, Falcone JF, Stengel PW, Cohen ML, Shannon HE, Gomeza J, Wess J, Felder CC. Investigations into the physiological role of muscarinic M2 and M4 muscarinic and M4 receptor subtypes using receptor knockout mice. Life Sci 2001;68:2473-2479. https:\/\/doi.org\/10.1016\/S0024-3205(01)01041-4","DOI":"10.1016\/S0024-3205(01)01041-4"},{"key":"ref46","doi-asserted-by":"crossref","unstructured":"47.\tWoolley ML, Carter HJ, Gartlon JE, Watson JM, Dawson LA. Attenuation of amphetamine-induced activity by the non-selective muscarinic receptor agonist, xanomeline, is absent in muscarinic M4 receptor knockout mice and attenuated in muscarinic M1 receptor knockout mice. Eur J Pharmacol 2009;603:147-149. https:\/\/doi.org\/10.1016\/j.ejphar.2008.12.020","DOI":"10.1016\/j.ejphar.2008.12.020"},{"key":"ref47","doi-asserted-by":"crossref","unstructured":"48.\tTurner J, Hughes LF, Toth LA. Sleep, activity, temperature and arousal responses of mice deficient for muscarinic receptor M2 or M4. Life Sci 2010;86:158-169. https:\/\/doi.org\/10.1016\/j.lfs.2009.11.019","DOI":"10.1016\/j.lfs.2009.11.019"},{"key":"ref48","doi-asserted-by":"crossref","unstructured":"49.\tRoedel A, Storch C, Holsboer F, Ohl F. Effects of light or dark phase testing on behavioural and cognitive performance in DBA mice. Lab Anim 2006;40:371-381. https:\/\/doi.org\/10.1258\/002367706778476343","DOI":"10.1258\/002367706778476343"},{"key":"ref49","unstructured":"50.\tCzeisler CA, Klerman EB. Circadian and sleep-dependent regulation of hormone release in humans. Recent Prog Horm Res 1999;54:97-130; discussion 130-132."},{"key":"ref50","doi-asserted-by":"crossref","unstructured":"51.\tEbling FJ, Lincoln GA, Wollnik F, Anderson N. Effects of constant darkness and constant light on circadian organization and reproductive responses in the ram. J Biol Rhythms 1988;3:365-384. https:\/\/doi.org\/10.1177\/074873048800300406","DOI":"10.1177\/074873048800300406"},{"key":"ref51","doi-asserted-by":"crossref","unstructured":"52.\tPatton AP, Hastings MH. The Mammalian Circadian Time-Keeping System. J Huntingtons Dis 2023;12:91-104. https:\/\/doi.org\/10.3233\/JHD-230571","DOI":"10.3233\/JHD-230571"},{"key":"ref52","doi-asserted-by":"crossref","unstructured":"53.\tJoye DAM, Evans JA. Sex differences in daily timekeeping and circadian clock circuits. Semin Cell Dev Biol 2022;126:45-55. https:\/\/doi.org\/10.1016\/j.semcdb.2021.04.026","DOI":"10.1016\/j.semcdb.2021.04.026"},{"key":"ref53","doi-asserted-by":"crossref","unstructured":"54.\tCzeisler CA, Duffy JF, Shanahan TL, Brown EN, Mitchell JF, Rimmer DW, Ronda JM, et al. Stability, precision, and near-24-hour period of the human circadian pacemaker. Science 1999;284:2177-2181. https:\/\/doi.org\/10.1126\/science.284.5423.2177","DOI":"10.1126\/science.284.5423.2177"},{"key":"ref54","unstructured":"55.\tGillette MU, Tischkau SA. Suprachiasmatic nucleus: the brain's circadian clock. Recent Prog Horm Res 1999;54:33-58; discussion 58-39."},{"key":"ref55","doi-asserted-by":"crossref","unstructured":"56.\tVosko AM, Colwell CS, Avidan AY. Jet lag syndrome: circadian organization, pathophysiology, and management strategies. Nat Sci Sleep 2010;2:187-198. https:\/\/doi.org\/10.2147\/NSS.S6683","DOI":"10.2147\/NSS.S6683"},{"key":"ref56","doi-asserted-by":"crossref","unstructured":"57.\tWyartt C. Taking a Big Step towards Understanding Locomotion. Trends Neurosci 2018;41:869-870. https:\/\/doi.org\/10.1016\/j.tins.2018.09.010","DOI":"10.1016\/j.tins.2018.09.010"},{"key":"ref57","doi-asserted-by":"crossref","unstructured":"58.\tBallesta A, Innominato PF, Dallmann R, Rand DA, L\u00e9vi FA. Systems Chronotherapeutics. Pharmacol Rev 2017;69:161-199. https:\/\/doi.org\/10.1124\/pr.116.013441","DOI":"10.1124\/pr.116.013441"},{"key":"ref58","doi-asserted-by":"crossref","unstructured":"59.\tMorin LP. Neuroanatomy of the extended circadian rhythm system. Expl Neurol 2013;243:4-20. https:\/\/doi.org\/10.1016\/j.expneurol.2012.06.026","DOI":"10.1016\/j.expneurol.2012.06.026"},{"key":"ref59","doi-asserted-by":"crossref","unstructured":"60.\tHughes ATL, Piggins HD. Feedback actions of locomotor activity to the circadian clock. Prog Brain Res 2012;199:305-336. https:\/\/doi.org\/10.1016\/B978-0-444-59427-3.00018-6","DOI":"10.1016\/B978-0-444-59427-3.00018-6"},{"key":"ref60","doi-asserted-by":"crossref","unstructured":"61.\tAbrahamson EE, Moore RY. Lesions of suprachiasmatic nucleus efferents selectively affect rest-activity rhythm. Mol Cell Endocrinol 2006;252:46-56. https:\/\/doi.org\/10.1016\/j.mce.2006.03.036","DOI":"10.1016\/j.mce.2006.03.036"},{"key":"ref61","doi-asserted-by":"crossref","unstructured":"62.\tKramer A, Yang F-C, Snodgrass P, Li X, Scammell TE, Davis FC, Weitz CJ. Regulation of Daily Locomotor Activity and Sleep by Hypothalamic EGF Receptor Signaling. Science 2001;294:2511-2515. https:\/\/doi.org\/10.1126\/science.1067716","DOI":"10.1126\/science.1067716"},{"key":"ref62","doi-asserted-by":"crossref","unstructured":"63.\tHut RA, Van der Zee EA. The cholinergic system, circadian rhythmicity, and time memory. Behav Brain Res 2011;221:466-480. https:\/\/doi.org\/10.1016\/j.bbr.2010.11.039","DOI":"10.1016\/j.bbr.2010.11.039"},{"key":"ref63","doi-asserted-by":"crossref","unstructured":"64.\tvan den Pol AN, Tsujimoto KL. Neurotransmitters of the hypothalamic suprachiasmatic nucleus: Immunocytochemical analysis of 25 neuronal antigens. Neuroscience 1985;15:1049-1086. https:\/\/doi.org\/10.1016\/0306-4522(85)90254-4","DOI":"10.1016\/0306-4522(85)90254-4"},{"key":"ref64","doi-asserted-by":"crossref","unstructured":"65.\tBina KG, Rusak B, Semba K. Localization of cholinergic neurons in the forebrain and brainstem that project to the suprachiasmatic nucleus of the hypothalamus in rat. J Comp Neurol 1993;335:295-307. https:\/\/doi.org\/10.1002\/cne.903350212","DOI":"10.1002\/cne.903350212"},{"key":"ref65","doi-asserted-by":"crossref","unstructured":"66.\tYang J-J, Wang Y-T, Cheng P-C, Kuo Y-J, Huang R-C. Cholinergic Modulation of Neuronal Excitability in the Rat Suprachiasmatic Nucleus. J Neurophysiol 2010;103:1397-1409. https:\/\/doi.org\/10.1152\/jn.00877.2009","DOI":"10.1152\/jn.00877.2009"},{"key":"ref66","doi-asserted-by":"crossref","unstructured":"67.\tEdelstein K, Amir S. Constant light induces persistent Fos expression in rat intergeniculate leaflet. Brain Res 1996;731:221-225. https:\/\/doi.org\/10.1016\/0006-8993(96)00691-9","DOI":"10.1016\/0006-8993(96)00691-9"},{"key":"ref67","doi-asserted-by":"crossref","unstructured":"68.\tMorin LP, Allen CN. The circadian visual system, 2005. Brain Res Rev 2006;51:1-60. https:\/\/doi.org\/10.1016\/j.brainresrev.2005.08.003","DOI":"10.1016\/j.brainresrev.2005.08.003"},{"key":"ref68","doi-asserted-by":"crossref","unstructured":"69.\tMoore RY. Chapter 8 Entrainment pathways and the functional organization of the circadian system. In: Progress in Brain Research. Buijs RM, Kalsbeek A, Romijn HJ, Pennartz CMA, Mirmiran M (eds), Elsevier, 1996, pp 103-119. https:\/\/doi.org\/10.1016\/S0079-6123(08)60403-3","DOI":"10.1016\/S0079-6123(08)60403-3"},{"key":"ref69","doi-asserted-by":"crossref","unstructured":"70.\tWatts AG, Swanson LW, Sanchez-Watts G. Efferent projections of the suprachiasmatic nucleus: I. Studies using anterograde transport of Phaseolus vulgaris leucoagglutinin in the rat. J Comp Neurol 1987;258:204-229. https:\/\/doi.org\/10.1002\/cne.902580204","DOI":"10.1002\/cne.902580204"},{"key":"ref70","doi-asserted-by":"crossref","unstructured":"71.\tWatts AG, Swanson LW. Efferent projections of the suprachiasmatic nucleus: II. Studies using retrograde transport of fluorescent dyes and simultaneous peptide immunohistochemistry in the rat. J Comp Neurol 1987;258:230-252. https:\/\/doi.org\/10.1002\/cne.902580205","DOI":"10.1002\/cne.902580205"},{"key":"ref71","doi-asserted-by":"crossref","unstructured":"72.\tKrout KE, Kawano J, Mettenleiter TC, Loewy AD. CNS inputs to the suprachiasmatic nucleus of the rat. Neuroscience 2002;110:73-92. https:\/\/doi.org\/10.1016\/S0306-4522(01)00551-6","DOI":"10.1016\/S0306-4522(01)00551-6"},{"key":"ref72","doi-asserted-by":"crossref","unstructured":"73.\tVujovic N, Gooley JJ, Jhou TC, Saper CB. Projections from the subparaventricular zone define four channels of output from the circadian timing system. J Comp Neurol 2015;523:2714-2737. https:\/\/doi.org\/10.1002\/cne.23812","DOI":"10.1002\/cne.23812"},{"key":"ref73","doi-asserted-by":"crossref","unstructured":"74.\tMoga MM, Weis RP, Moore RY. Efferent projections of the paraventricular thalamic nucleus in the rat. J Comp Neurol 1995;359:221-238. https:\/\/doi.org\/10.1002\/cne.903590204","DOI":"10.1002\/cne.903590204"},{"key":"ref74","doi-asserted-by":"crossref","unstructured":"75.\tCastillo-Ruiz A, Nunez AA. Cholinergic projections to the suprachiasmatic nucleus and lower subparaventricular zone of diurnal and nocturnal rodents. Brain Res 2007;1151:91-101. https:\/\/doi.org\/10.1016\/j.brainres.2007.03.010","DOI":"10.1016\/j.brainres.2007.03.010"},{"key":"ref75","doi-asserted-by":"crossref","unstructured":"76.\tDeurveilher S, Semba K. Indirect projections from the suprachiasmatic nucleus to major arousal-promoting cell groups in rat: Implications for the circadian control of behavioural state. Neuroscience 2005;130:165-183. https:\/\/doi.org\/10.1016\/j.neuroscience.2004.08.030","DOI":"10.1016\/j.neuroscience.2004.08.030"},{"key":"ref76","doi-asserted-by":"crossref","unstructured":"77.\tRiljak V, Janisova K, Myslivecek J. Lack of M(4) muscarinic receptors in the striatum, thalamus and intergeniculate leaflet alters the biological rhythm of locomotor activity in mice. Brain Struct Funct 2020;225:1615-1629. https:\/\/doi.org\/10.1007\/s00429-020-02082-x","DOI":"10.1007\/s00429-020-02082-x"},{"key":"ref77","doi-asserted-by":"crossref","unstructured":"78.\tValuskova P, Farar V, Forczek S, Krizova I, Myslivecek J. Autoradiography of (3)H-pirenzepine and (3)H-AFDX-384 in Mouse Brain Regions: Possible Insights into M(1), M(2), and M(4) Muscarinic Receptors Distribution. Front Pharmacol 2018;9:124. https:\/\/doi.org\/10.3389\/fphar.2018.00124","DOI":"10.3389\/fphar.2018.00124"},{"key":"ref78","doi-asserted-by":"crossref","unstructured":"79.\tPerry EK, Perry RH, Tomlinson BE. Circadian variations in cholinergic enzymes and muscarinic receptor binding in human cerebral cortex. Neurosci Lett 1977;4:185-189. https:\/\/doi.org\/10.1016\/0304-3940(77)90136-7","DOI":"10.1016\/0304-3940(77)90136-7"},{"key":"ref79","unstructured":"80.\tKafka MS, Wirz-Justice A, Naber D, Moore RY, Benedito MA. Circadian rhythms in rat brain neurotransmitter receptors. Fed Proc 1983;42:2796-2801."},{"key":"ref80","doi-asserted-by":"crossref","unstructured":"81.\tWirz-Justice A. Circadian rhythms in mammalian neurotransmitter receptors. Progress Neurobiol 1987;29:219-259. https:\/\/doi.org\/10.1016\/0301-0082(87)90022-0","DOI":"10.1016\/0301-0082(87)90022-0"},{"key":"ref81","doi-asserted-by":"crossref","unstructured":"82.\tPor S, Bondy S. Regional circadian variation of acetylcholine muscarinic receptors in the rat brain. J Neurosci Res 1981;6:315-318. https:\/\/doi.org\/10.1002\/jnr.490060306","DOI":"10.1002\/jnr.490060306"},{"key":"ref82","doi-asserted-by":"crossref","unstructured":"83.\tKafka MS, Benedito MA, Blendy JA, Tokola NS. Circadian rhythms in neurotransmitter receptors in discrete rat brain regions. Chronobiol Int 1986;3:91-100. https:\/\/doi.org\/10.3109\/07420528609066353","DOI":"10.3109\/07420528609066353"},{"key":"ref83","doi-asserted-by":"crossref","unstructured":"84.\tMarquez E, Pavia J, Laukonnen S, Martos F, Gomez A, Rius F, Sanchez de la Cuesta F. Circadian rhythm in muscarinic receptor subtypes in rat forebrain. Chronobiol Int 1990;7:277-282. https:\/\/doi.org\/10.1080\/07420529009064633","DOI":"10.1080\/07420529009064633"},{"key":"ref84","doi-asserted-by":"crossref","unstructured":"85.\tBina KG, Rusak B, Wilkinson M. Daily variation of muscarinic receptors in visual cortex but not suprachiasmatic nucleus of Syrian hamsters. Brain Res 1998;797:143-153. https:\/\/doi.org\/10.1016\/S0006-8993(98)00374-6","DOI":"10.1016\/S0006-8993(98)00374-6"},{"key":"ref85","doi-asserted-by":"crossref","unstructured":"86.\tBailey M, Silver R. Sex differences in circadian timing systems: Implications for disease. Front Neuroendocrinol 2014;35:111-139. https:\/\/doi.org\/10.1016\/j.yfrne.2013.11.003","DOI":"10.1016\/j.yfrne.2013.11.003"},{"key":"ref86","doi-asserted-by":"crossref","unstructured":"87.\tSimerly RB. Wired on hormones: endocrine regulation of hypothalamic development. Curr Opin Neurobiol 2005;15:81-85. https:\/\/doi.org\/10.1016\/j.conb.2005.01.013","DOI":"10.1016\/j.conb.2005.01.013"},{"key":"ref87","doi-asserted-by":"crossref","unstructured":"88.\tMcCarthy MM, Arnold AP, Ball GF, Blaustein JD, De Vries GJ. Sex differences in the brain: the not so inconvenient truth. J Neurosci 2012;32:2241-2247. https:\/\/doi.org\/10.1523\/JNEUROSCI.5372-11.2012","DOI":"10.1523\/JNEUROSCI.5372-11.2012"},{"key":"ref88","doi-asserted-by":"crossref","unstructured":"89.\tInstitute of Medicine (US) Forum on Neuroscience and Nervous System Disorders. The National Academies Collection: Reports funded by National Institutes of Health. Sex Differences and Implications for Translational Neuroscience Research: Workshop Summary. Washington (DC): National Academies Press (US); 2011. https:\/\/doi.org\/10.17226\/13004","DOI":"10.17226\/13004"},{"key":"ref89","doi-asserted-by":"crossref","unstructured":"90.\tMorgan MA, Pfaff DW. Effects of estrogen on activity and fear-related behaviors in mice. Horm Behav 2001;40:472-482. https:\/\/doi.org\/10.1006\/hbeh.2001.1716","DOI":"10.1006\/hbeh.2001.1716"},{"key":"ref90","doi-asserted-by":"crossref","unstructured":"91.\tBlizard DA, Lippman HR, Chen JJ. Sex differences in open-field behavior in the rat: the inductive and activational role of gonadal hormones. Physiol Behav 1975;14:601-608. https:\/\/doi.org\/10.1016\/0031-9384(75)90188-2","DOI":"10.1016\/0031-9384(75)90188-2"},{"key":"ref91","doi-asserted-by":"crossref","unstructured":"92.\tKuljis DA, Loh DH, Truong D, Vosko AM, Ong ML, McClusky R, Arnold AP, Colwell CS. Gonadal- and sex-chromosome-dependent sex differences in the circadian system. Endocrinology 2013;154:1501-1512. https:\/\/doi.org\/10.1210\/en.2012-1921","DOI":"10.1210\/en.2012-1921"},{"key":"ref92","doi-asserted-by":"crossref","unstructured":"93.\tOgawa S, Chan J, Gustafsson J-A, Korach KS, Pfaff DW. Estrogen increases locomotor activity in mice through estrogen receptor alpha: specificity for the type of activity. Endocrinology 2003;144:230-239. https:\/\/doi.org\/10.1210\/en.2002-220519","DOI":"10.1210\/en.2002-220519"},{"key":"ref93","doi-asserted-by":"crossref","unstructured":"94.\tGibbs RB. Effects of gonadal hormone replacement on measures of basal forebrain cholinergic function. Neuroscience 2000;101:931-938. https:\/\/doi.org\/10.1016\/S0306-4522(00)00433-4","DOI":"10.1016\/S0306-4522(00)00433-4"},{"key":"ref94","doi-asserted-by":"crossref","unstructured":"95.\tLuine VN, Renner KJ, Heady S, Jones KJ. Age and sex-dependent decreases in ChAT in basal forebrain nuclei. Neurobiol Aging 1986;7:193-198. https:\/\/doi.org\/10.1016\/0197-4580(86)90042-4","DOI":"10.1016\/0197-4580(86)90042-4"},{"key":"ref95","doi-asserted-by":"crossref","unstructured":"96.\tWestlind-Danielsson A, Gould E, McEwen BS. Thyroid hormone causes sexually distinct neurochemical and morphological alterations in rat septal-diagonal band neurons. J Neurochem 1991;56:119-128. https:\/\/doi.org\/10.1111\/j.1471-4159.1991.tb02570.x","DOI":"10.1111\/j.1471-4159.1991.tb02570.x"},{"key":"ref96","doi-asserted-by":"crossref","unstructured":"97.\tGorski RA, Gordon JH, Shryne JE, Southam AM. Evidence for a morphological sex difference within the medial preoptic area of the rat brain. Brain Res 1978;148:333-346. https:\/\/doi.org\/10.1016\/0006-8993(78)90723-0","DOI":"10.1016\/0006-8993(78)90723-0"},{"key":"ref97","doi-asserted-by":"crossref","unstructured":"98.\tFragkouli A, Stamatakis A, Zographos E, Pachnis V, Stylianopoulou F. Sexually dimorphic effects of the Lhx7 null mutation on forebrain cholinergic function. Neuroscience 2006;137:1153-1164. https:\/\/doi.org\/10.1016\/j.neuroscience.2005.10.037","DOI":"10.1016\/j.neuroscience.2005.10.037"},{"key":"ref98","doi-asserted-by":"crossref","unstructured":"99.\tShughrue P, Scrimo P, Lane M, Askew R, Merchenthaler I. The distribution of estrogen receptor-beta mRNA in forebrain regions of the estrogen receptor-alpha knockout mouse. Endocrinology 1997;138:5649-5652. https:\/\/doi.org\/10.1210\/endo.138.12.5712","DOI":"10.1210\/endo.138.12.5712"},{"key":"ref99","doi-asserted-by":"crossref","unstructured":"100.\tValuskova P, Riljak V, Forczek ST, Farar V, Myslivecek J. Variability in the Drug Response of M4 Muscarinic Receptor Knockout Mice During Day and Night Time. Front Pharmacol 2019;10. https:\/\/doi.org\/10.3389\/fphar.2019.00237","DOI":"10.3389\/fphar.2019.00237"},{"key":"ref100","doi-asserted-by":"crossref","unstructured":"101.\tValuskova P, Forczek ST, Farar V, Myslivecek J. The deletion of M(4) muscarinic receptors increases motor activity in females in the dark phase. Brain Behav 2018;8:e01057. https:\/\/doi.org\/10.1002\/brb3.1057","DOI":"10.1002\/brb3.1057"},{"key":"ref101","doi-asserted-by":"crossref","unstructured":"102.\tJanisova K, Uhlirova M, Forczek S, Myslivecek J. Striatal M4 muscarinic receptors determine the biological rhythm of activity, with a supportive role of M1 muscarinic receptors. Front Pharmacol 2025;16:1691118. https:\/\/doi.org\/10.3389\/fphar.2025.1691118","DOI":"10.3389\/fphar.2025.1691118"},{"key":"ref102","doi-asserted-by":"crossref","unstructured":"103.\tNair AG, Castro LRV, El Khoury M, Gorgievski V, Giros B, Tzavara ET, Hellgren-Kotaleski J, Vincent P. The high efficacy of muscarinic M4 receptor in D1 medium spiny neurons reverses striatal hyperdopaminergia. Neuropharmacology 2019;146:74-83. https:\/\/doi.org\/10.1016\/j.neuropharm.2018.11.029","DOI":"10.1016\/j.neuropharm.2018.11.029"},{"key":"ref103","doi-asserted-by":"crossref","unstructured":"104.\tSarter M, Albin RL, Kucinski A, Lustig C. Where attention falls: Increased risk of falls from the converging impact of cortical cholinergic and midbrain dopamine loss on striatal function. Exp Neurol 2014;257:120-129. https:\/\/doi.org\/10.1016\/j.expneurol.2014.04.032","DOI":"10.1016\/j.expneurol.2014.04.032"},{"key":"ref104","doi-asserted-by":"crossref","unstructured":"105.\tMartins-Silva C, De Jaeger X, Guzman MS, Lima RDF, Santos MS, Kushmerick C, Gomez MV, et al. Novel Strains of Mice Deficient for the Vesicular Acetylcholine Transporter: Insights on Transcriptional Regulation and Control of Locomotor Behavior. PLoS One 2011;6:e17611. https:\/\/doi.org\/10.1371\/journal.pone.0017611","DOI":"10.1371\/journal.pone.0017611"},{"key":"ref105","doi-asserted-by":"crossref","unstructured":"106.\tGuzman MS, De Jaeger X, Raulic S, Souza IA, Li AX, Schmid S, Menon RS, et al. Elimination of the Vesicular Acetylcholine Transporter in the Striatum Reveals Regulation of Behaviour by Cholinergic-Glutamatergic Co-Transmission. PLoS Biol 2011;9:e1001194. https:\/\/doi.org\/10.1371\/journal.pbio.1001194","DOI":"10.1371\/journal.pbio.1001194"},{"key":"ref106","doi-asserted-by":"crossref","unstructured":"107.\tMiyakawa T, Yamada M, Duttaroy A, Wess J. Hyperactivity and Intact Hippocampus-Dependent Learning in Mice Lacking the M1 Muscarinic Acetylcholine Receptor. J Neurosci 2001;21:5239-5250. https:\/\/doi.org\/10.1523\/JNEUROSCI.21-14-05239.2001","DOI":"10.1523\/JNEUROSCI.21-14-05239.2001"},{"key":"ref107","doi-asserted-by":"crossref","unstructured":"108.\tZhang W, Yamada M, Gomeza J, Basile AS, Wess J. Multiple muscarinic acetylcholine receptor subtypes modulate striatal dopamine release, as studied with M1-M5 muscarinic receptor knock-out mice. J Neurosci 2002;22:6347-6352. https:\/\/doi.org\/10.1523\/JNEUROSCI.22-15-06347.2002","DOI":"10.1523\/JNEUROSCI.22-15-06347.2002"},{"key":"ref108","doi-asserted-by":"crossref","unstructured":"109.\tJeon J, Dencker D, W\u00f6rtwein G, Woldbye DPD, Cui Y, Davis AA, Levey AI, et al. A Subpopulation of Neuronal M4 Muscarinic Acetylcholine Receptors Plays a Critical Role in Modulating Dopamine-Dependent Behaviors. J Neurosci 2010;30:2396-2405. https:\/\/doi.org\/10.1523\/JNEUROSCI.3843-09.2010","DOI":"10.1523\/JNEUROSCI.3843-09.2010"},{"key":"ref109","doi-asserted-by":"crossref","unstructured":"110.\tMoehle MS, Conn PJ. Roles of the M4 acetylcholine receptor in the basal ganglia and the treatment of movement disorders. Mov Disord 2019;34:1089-1099. https:\/\/doi.org\/10.1002\/mds.27740","DOI":"10.1002\/mds.27740"},{"key":"ref110","doi-asserted-by":"crossref","unstructured":"111.\tMann T, Zilles K, Klawitter F, Cremer M, Hawlitschka A, Palomero-Gallagher N, Schmitt O, Wree A. Acetylcholine Neurotransmitter Receptor Densities in the Striatum of Hemiparkinsonian Rats Following Botulinum Neurotoxin-A Injection. Front Neuroanat 2018;12:65. https:\/\/doi.org\/10.3389\/fnana.2018.00065","DOI":"10.3389\/fnana.2018.00065"},{"key":"ref111","doi-asserted-by":"crossref","unstructured":"112.\tOuchi H, Ono K, Murakami Y, Matsumoto K. Social isolation induces deficit of latent learning performance in mice: a putative animal model of attention deficit\/hyperactivity disorder. Behav Brain Res 2013;238:146-153. https:\/\/doi.org\/10.1016\/j.bbr.2012.10.029","DOI":"10.1016\/j.bbr.2012.10.029"},{"key":"ref112","doi-asserted-by":"crossref","unstructured":"113.\tBartko SJ, Romberg C, White B, Wess J, Bussey TJ, Saksida LM. Intact attentional processing but abnormal responding in M1 muscarinic receptor-deficient mice using an automated touchscreen method. Neuropharmacology 2011;61:1366-1378. https:\/\/doi.org\/10.1016\/j.neuropharm.2011.08.023","DOI":"10.1016\/j.neuropharm.2011.08.023"},{"key":"ref113","doi-asserted-by":"crossref","unstructured":"114.\tTzavara ET, Bymaster FP, Davis RJ, Wade MR, Perry KW, Wess J, McKinzie DL, et al. M4 muscarinic receptors regulate the dynamics of cholinergic and dopaminergic neurotransmission: relevance to the pathophysiology and treatment of related CNS pathologies. FASEB J 2004;18:1410-1412. https:\/\/doi.org\/10.1096\/fj.04-1575fje","DOI":"10.1096\/fj.04-1575fje"},{"key":"ref114","doi-asserted-by":"crossref","unstructured":"115.\tJusko ML, Smith JN, Hayes T, Campez-Pardo M, Timmons AC, Morrow AS, Lozano C, et al. An Application of Time Series Analysis to Single-Case Designs in an Intensive Behavioral Intervention for ADHD. J Atten Disord 2025;29:832-847. https:\/\/doi.org\/10.1177\/10870547251339546","DOI":"10.1177\/10870547251339546"},{"key":"ref115","doi-asserted-by":"crossref","unstructured":"116.\tJo Y, Takagi S, Shimizu M, Takahashi H, Sugihara G. Seasonal changes in mood and behaviors in individuals with attention-deficit\/hyperactivity disorder trait. 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