{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T06:02:46Z","timestamp":1740808966751,"version":"3.38.0"},"reference-count":0,"publisher":"Aerospace Medical Association","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["aerosp med hum perform"],"published-print":{"date-parts":[[2019,2,1]]},"abstract":"<jats:p><jats:bold>BACKGROUND:<\/jats:bold> Space is a special environment in which microgravity and cosmic rays are the primary factors that induce gene mutations of microorganisms. In our previous studies, a single point mutation in the gene dprA was found in an <jats:italic>Enterococcus faecium<\/jats:italic> strain of LCT-EF258\n after spaceflight. DNA processing protein A (DprA) plays a prominent role in the horizontal transfer of genes among bacteria (such as <jats:italic>Streptococcus pneumoniae, Helicobacter pylori, Bacillus subtilis,<\/jats:italic> and <jats:italic>Rhodobacter capsulatus<\/jats:italic>). However, the function of DprA in <jats:italic>E. faecium<\/jats:italic>\n remains unknown. Furthermore, <jats:italic>E. faecium<\/jats:italic> could acquire antibiotic resistance through the horizontal transfer of antibiotic resistance genes, but it is unclear whether dprA mutants could affect this process in <jats:italic>E. faecium<\/jats:italic>.<jats:bold>METHODS:<\/jats:bold> In this study, we constructed a plasmid\n containing the vancomycin resistance gene vanA and then transferred the gene vanA into the dprA-mutant strain LCT-EF258 and the control strain LCT-EF90 using the electroporation technique. We then used Discovery Studio<jats:sup>TM<\/jats:sup> software to construct the 3D protein structure.<jats:bold>RESULTS:<\/jats:bold>\n The results showed that the horizontal transfer efficiency of the vancomycin resistance gene vanA in the dprA-mutant <jats:italic>E. faecium<\/jats:italic> decreased. And the hydrophobic core of the mutant DprA became stable and the binding affinity between the mutant DprA and ssDNA reduced.<jats:bold>DISCUSSION:<\/jats:bold>\n This study is an exploration of bacterial gene mutation after spaceflight. The dprA mutant could affect the ability of <jats:italic>E. faecium<\/jats:italic> to acquire exogenous resistance gene vanA, which offered us an interesting path to block the dissemination of resistance genes between strains.<jats:bold>Yu\n Y, Chang D, Guo Q, Wang J, Liu C. <jats:italic>LCT-EF258 with S171 mutation in DprA exhibits horizontal gene transfer deficiency after spaceflight<\/jats:italic>. Aerosp Med Hum Perform. 2019; 90(2):116\u2013122.<\/jats:bold><\/jats:p>","DOI":"10.3357\/amhp.5120.2019","type":"journal-article","created":{"date-parts":[[2019,1,23]],"date-time":"2019-01-23T04:54:51Z","timestamp":1548219291000},"page":"116-122","source":"Crossref","is-referenced-by-count":0,"title":["LCT-EF258 with S17I Mutation in DprA Exhibits Horizontal Gene Transfer Deficiency After Spaceflight"],"prefix":"10.3357","volume":"90","author":[{"given":"Yi","family":"Yu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"De","family":"Chang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiang","family":"Guo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junfeng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Changting","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1911","container-title":["Aerospace Medicine and Human Performance"],"original-title":[],"link":[{"URL":"https:\/\/asma.kglmeridian.com\/view\/journals\/amhp\/90\/2\/article-p116.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/asma.kglmeridian.com\/downloadpdf\/journals\/amhp\/90\/2\/article-p116.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/asma.kglmeridian.com\/downloadpdf\/journals\/amhp\/90\/2\/article-p116.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:02:37Z","timestamp":1740787357000},"score":1,"resource":{"primary":{"URL":"https:\/\/asma.kglmeridian.com\/view\/journals\/amhp\/90\/2\/article-p116.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,2,1]]},"references-count":0,"journal-issue":{"issue":"2"},"URL":"https:\/\/doi.org\/10.3357\/amhp.5120.2019","relation":{},"ISSN":["2375-6314","2375-6322"],"issn-type":[{"type":"print","value":"2375-6314"},{"type":"electronic","value":"2375-6322"}],"subject":[],"published":{"date-parts":[[2019,2,1]]}}}