{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T19:22:11Z","timestamp":1790018531846,"version":"4.0.1"},"reference-count":24,"publisher":"Aerospace Medical Association","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:p>\n                    <jats:bold>INTRODUCTION:<\/jats:bold>\n                    Understanding physiological responses to high G-load is important for ensuring flight safety. In this pilot study, to establish a methodology for detecting salivary microRNAs (miRNAs) in humans before and after high G-load, we examined the relationship between miRNAs and high G-load or G-induced loss of consciousness (G-LOC), referencing previous concussion studies.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>METHODS:<\/jats:bold>\n                    This study included 25 healthy pilot trainees attending centrifuge training for the primary course from June to October 2023. Saliva samples were collected at 3 time points: the day before centrifuge training (Pre), 3 h after training (Post1), and 20 h after training (Post2). Finally, miRNAs from 21 subjects, including 1 female, were analyzed using reverse transcription-quantitative polymerase chain reaction. Four miRNAs (miR-107, miR-34a-5p, miR-27a-5p, miR-30a-3p) were selected for analysis.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>RESULTS:<\/jats:bold>\n                    Salivary miRNAs from 4 of the 25 subjects could not be analyzed due to insufficient quality and quantity. Comparing the miRNA levels in 21 subjects at 3 time points, miR-107 at Post2 was significantly lower than that at Post1 (1.03 \u00b1 0.39 vs. 1.32 \u00b1 0.53). Of the 21 subjects, 16 did not experience G-LOC and 5 did. Comparing the levels between the no G-LOC and G-LOC groups at each point, only miR-34a-5p levels at Post1 were significantly different (1.11 \u00b1 0.48 vs. 0.65 \u00b1 0.11).\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>DISCUSSION:<\/jats:bold>\n                    The key findings of this study were: 1) miRNAs suitable for analysis were extracted from the majority of saliva samples (21\/25, 84%); and 2) changes in salivary miRNAs were detected by reverse transcription-quantitative polymerase chain reaction.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>\n                      Akiyama M, Takei F, Takada K.\n                      <jats:italic>A pilot study of salivary microRNA changes due to high G-load<\/jats:italic>\n                      . Aerosp Med Hum Perform. 2026; 97(2):106\u2013110.\n                    <\/jats:bold>\n                  <\/jats:p>","DOI":"10.3357\/amhp.6757.2026","type":"journal-article","created":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T13:44:31Z","timestamp":1770731071000},"page":"106-110","source":"Crossref","is-referenced-by-count":1,"title":["A Pilot Study of Salivary MicroRNA Changes Due to High G-Load"],"prefix":"10.3357","volume":"97","author":[{"given":"Misaki","family":"Akiyama","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fumie","family":"Takei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunio","family":"Takada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1911","reference":[{"issue":"1","key":"B1","series-title":"Aviat Space Environ Med.","first-page":"2","article-title":"G-induced loss of consciousness: definition, history, current status","volume":"59","author":"Burton RR.","year":"1988","unstructured":"Burton RR. G-induced loss of consciousness: definition, history, current status. Aviat Space Environ Med. 1988 Jan; 59(1):2\u20135."},{"key":"B2","series-title":"Sci Rep.","first-page":"15643","article-title":"A load of mice to hypergravity causes AMPK\u03b1 repression with liver injury, which is overcome by preconditioning loads via Nrf2","volume":"5","author":"Lee SG","year":"2015","unstructured":"Lee SG, Lee CG, Wu HM, Oh CS, Chung SW, Kim SG. A load of mice to hypergravity causes AMPK\u03b1 repression with liver injury, which is overcome by preconditioning loads via Nrf2. Sci Rep. 2015 Oct 23; 5:15643. Erratum in: Sci Rep. 2016 Oct 5; 6:34677."},{"issue":"2","key":"B3","series-title":"Mol Cell.","first-page":"205","article-title":"MicroRNA functions in stress responses","volume":"40","author":"Leung AKL","year":"2010","unstructured":"Leung AKL, Sharp PA. MicroRNA functions in stress responses. 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