{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T01:13:55Z","timestamp":1783646035111,"version":"3.55.0"},"reference-count":15,"publisher":"Aerospace Medical Association","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:p>\n                    <jats:bold>INTRODUCTION:<\/jats:bold>\n                    A lumbar puncture (LP) is a diagnostic and therapeutic procedure for wide-ranging conditions terrestrially with additional applications in the microgravity environment of human spaceflight. LP has been performed on astronauts postflight in an attempt to answer lingering health questions, like the causes of spaceflight-associated neuro-ocular syndrome. However, an LP has not been performed in space or microgravity before due to the operational constraints of the procedure, including infection risk, air entrance, and fluid management difficulties in microgravity. This has prevented in-flight intracranial pressure measurements and important characterizations of cerebrospinal fluid (CSF) composition during spaceflight.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>METHODS:<\/jats:bold>\n                    We developed a closed-system LP design by introducing an injection port between the typical needle-stylet LP device and using an electronic pressure transducer (eliminating gravity-based manometry). An initial proof of concept demonstration of the new device was conducted by performing mock LPs using an LP trainer with known fluid pressures.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>RESULTS:<\/jats:bold>\n                    Closed-system LP was successfully demonstrated with no loss of fluid external to the system and no appreciable entrance of air. The new closed system showed accurate (\n                    <jats:italic>r<\/jats:italic>\n                    = 0.995) and precise (SD = 0.406 mmHg) results when measuring pressures in the typical CSF range of 8\u201315 mmHg.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>DISCUSSION:<\/jats:bold>\n                    This initial demonstration serves as the first step in testing a novel LP system that mitigates risks of conducting the procedure during spaceflight. An in-flight LP would enable the collection of vital data on intracranial pressure and CSF composition under microgravity. Development of custom equipment and in vivo testing are needed for further validation.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>\n                      Adams B, Levinson Z, Alexandrov P, Mampre D.\n                      <jats:italic>A closed-system lumbar puncture design for microgravity<\/jats:italic>\n                      . Aerosp Med Hum Perform. 2026; 97(7):534\u2013538.\n                    <\/jats:bold>\n                  <\/jats:p>","DOI":"10.3357\/amhp.6853.2026","type":"journal-article","created":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T00:30:29Z","timestamp":1783643429000},"page":"534-538","source":"Crossref","is-referenced-by-count":0,"title":["A Closed-System Lumbar Puncture Design for Microgravity"],"prefix":"10.3357","volume":"97","author":[{"given":"Brandon","family":"Adams","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zoey","family":"Levinson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Alexandrov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Mampre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1911","reference":[{"issue":"12","key":"B1","series-title":"JAMA Ophthalmol.","first-page":"1193","article-title":"Identification of factors associated with the development of optic disc edema during spaceflight","volume":"140","author":"Pardon LP","year":"2022","unstructured":"Pardon LP, Greenwald SH, Ferguson CR, Patel NB, Young M,  Identification of factors associated with the development of optic disc edema during spaceflight. JAMA Ophthalmol. 2022 Dec 1; 140(12):1193\u2013200."},{"key":"B2","series-title":"Evidence report: risk of Spaceflight Associated Neuro-ocular Syndrome (SANS).","author":"Laurie S","year":"2022","unstructured":"Laurie S, Macias B, Pardon L, Brunstetter T, Tarver W,  Evidence report: risk of Spaceflight Associated Neuro-ocular Syndrome (SANS). Houston, TX: Human Research Program, Human Health Countermeasures Element; 2022."},{"issue":"9","key":"B3","series-title":"Brain Behav.","first-page":"e3648","article-title":"Simulated microgravity-induced dysregulation of cerebrospinal fluid immune homeostasis by disrupting the blood-cerebrospinal fluid barrier","volume":"14","author":"Yang J","year":"2024","unstructured":"Yang J, Cui Y, Zhao J, Tang S, Wang A,  Simulated microgravity-induced dysregulation of cerebrospinal fluid immune homeostasis by disrupting the blood-cerebrospinal fluid barrier. Brain Behav. 2024 Sept; 14(9):e3648."},{"issue":"6","key":"B4","series-title":"J Assoc Res Otolaryngol.","first-page":"563","article-title":"Otoacoustic estimate of astronauts\u2019 intracranial pressure changes during spaceflight","volume":"25","author":"Moleti A","year":"2024","unstructured":"Moleti A, Minniti T, Sharma Y, Russo A, Civiero A,  Otoacoustic estimate of astronauts\u2019 intracranial pressure changes during spaceflight. J Assoc Res Otolaryngol. 2024 Dec; 25(6):563\u201373."},{"key":"B5","series-title":"J Physiol.","first-page":"1067","article-title":"Long-duration spaceflight alters estimated intracranial pressure and cerebral blood velocity","volume":"599","author":"Iwasaki K-I","year":"2021","unstructured":"Iwasaki K-I, Ogawa Y, Kurazumi T, Imaduddin SM, Mukai C,  Long-duration spaceflight alters estimated intracranial pressure and cerebral blood velocity. J Physiol. 2021 Feb; 599(4):1067\u201381."},{"issue":"1","key":"B6","series-title":"Aviat Space Environ Med.","first-page":"3","article-title":"Doppler ultrasound of the central retinal artery in microgravity","volume":"85","author":"Sirek AS","year":"2014","unstructured":"Sirek AS, Garcia K, Foy M, Ebert D, Sargsyan A,  Doppler ultrasound of the central retinal artery in microgravity. Aviat Space Environ Med. 2014 Jan; 85(1):3\u20138."},{"issue":"6","key":"B7","series-title":"Aerosp Med Hum Perform.","first-page":"496","article-title":"Spaceflight Associated Neuro-ocular Syndrome as a potential variant of venous overload choroidopathy","volume":"96","author":"Mampre D","year":"2025","unstructured":"Mampre D, Spaide R, Mason S, Van Baalen M, Gibson CR,  Spaceflight Associated Neuro-ocular Syndrome as a potential variant of venous overload choroidopathy. Aerosp Med Hum Perform. 2025 Jun; 96(6):496\u2013508."},{"issue":"2","key":"B8","series-title":"Neurology.","first-page":"155","article-title":"CSF pressure assessed by lumbar puncture agrees with intracranial pressure","volume":"68","author":"Lenfeldt N","year":"2007","unstructured":"Lenfeldt N, Koskinen LO, Bergenheim AT, Malm J, Eklund A. CSF pressure assessed by lumbar puncture agrees with intracranial pressure. Neurology. 2007 Jan 9; 68(2):155\u20138."},{"key":"B9","series-title":"NPJ Microgravity.","first-page":"7","article-title":"Spaceflight Associated Neuro-ocular Syndrome (SANS) and the neuro-ophthalmologic effects of microgravity: a review and an update","volume":"6","author":"Lee AG","year":"2020","unstructured":"Lee AG, Mader TH, Gibson CR, Tarver W, Rabiei P,  Spaceflight Associated Neuro-ocular Syndrome (SANS) and the neuro-ophthalmologic effects of microgravity: a review and an update. NPJ Microgravity. 2020 Feb 7; 6:7. Erratum in: NPJ Microgravity. 2020 Aug 26; 6:23."},{"issue":"8","key":"B10","series-title":"Aerosp Med Hum Perform.","first-page":"745","article-title":"Technique for performing lumbar puncture in microgravity using portable radiography","volume":"87","author":"Lerner DJ","year":"2016","unstructured":"Lerner DJ, Parmet AJ, Don S, Shimony JS, Goyal MS. Technique for performing lumbar puncture in microgravity using portable radiography. Aerosp Med Hum Perform. 2016 Aug; 87(8):745\u20137."},{"key":"B11","article-title":"Ultrasound-guided lumbar puncture with AI-based image labeling for intracranial pressure assessment in spaceflight","author":"Sarago\u00e7a B.","year":"2024","unstructured":"Sarago\u00e7a B. Ultrasound-guided lumbar puncture with AI-based image labeling for intracranial pressure assessment in spaceflight [Master\u2019s Thesis]. Lisbon, Portugal: Instituto Superior T\u00e9cnico; 2024."},{"issue":"12","key":"B12","series-title":"Aviat Space Environ Med.","first-page":"1209","article-title":"Lumbar puncture during spaceflight: operational considerations, constraints, concerns, and limitations","volume":"85","author":"Barr YR.","year":"2014","unstructured":"Barr YR. Lumbar puncture during spaceflight: operational considerations, constraints, concerns, and limitations. Aviat Space Environ Med. 2014 Dec; 85(12):1209\u201313."},{"key":"B13","series-title":"NASA Tech Briefs.","first-page":"23","article-title":"\u201cZero-mass\u201d noninvasive pressure transducers","author":"Hartley FT.","year":"2009","unstructured":"Hartley FT. \u201cZero-mass\u201d noninvasive pressure transducers. NASA Tech Briefs. La Ca\u00f1ada Flintridge (CA): NASA Jet Propulsion Laboratory; May 2009:23."},{"key":"B14","article-title":"The Entry Descent and Landing Instrumentation (MEDLI2) Suite for the Mars 2020 mission. Poster session presented at: Science Instrumentation, Experiments, and In-Situ Measurements","author":"White T","year":"2019","unstructured":"White T, Mahzari M, Tang C, Karlgaard CD, Schoenberger M, Cruden BA. The Entry Descent and Landing Instrumentation (MEDLI2) Suite for the Mars 2020 mission. Poster session presented at: Science Instrumentation, Experiments, and In-Situ Measurements. 16th International Planetary Probe Workshop; 2019 July 8\u201312; Oxford University, UK. [Accessed 14 May 2026]. Available through the IPPW Archive at https:\/\/drive.google.com\/drive\/folders\/1GrwrzeF-pV59ejqpXJwMBpBCHF6vi435."},{"key":"B15","series-title":"StatPearls.","article-title":"Neuroanatomy, cerebrospinal fluid","author":"Konstantinos M","year":"2025","unstructured":"Konstantinos M, Weisbrod LJ, Launico MV. Neuroanatomy, cerebrospinal fluid. In: StatPearls. Treasure Island (FL): StatPearls Publishing; 2025 Jul."}],"container-title":["Aerospace Medicine and Human Performance"],"original-title":[],"link":[{"URL":"https:\/\/asma.kglmeridian.com\/view\/journals\/amhp\/97\/7\/article-p534.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/asma.kglmeridian.com\/downloadpdf\/journals\/amhp\/97\/7\/article-p534.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/asma.kglmeridian.com\/downloadpdf\/journals\/amhp\/97\/7\/article-p534.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T00:30:52Z","timestamp":1783643452000},"score":1,"resource":{"primary":{"URL":"https:\/\/asma.kglmeridian.com\/view\/journals\/amhp\/97\/7\/article-p534.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7]]},"references-count":15,"journal-issue":{"issue":"7"},"URL":"https:\/\/doi.org\/10.3357\/amhp.6853.2026","relation":{},"ISSN":["2375-6314","2375-6322"],"issn-type":[{"value":"2375-6314","type":"print"},{"value":"2375-6322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7]]}}}