{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T15:56:31Z","timestamp":1723046191035},"reference-count":0,"publisher":"Cell Physiol Biochem Press GmbH and Co KG","issue":"S1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Physiol Biochem"],"abstract":"<jats:p>BACKGROUND\/AIMS:  The osmolytes involved in the volume regulation of hyposmotically-swollen fish cells are well identified. However, if a coordination and adjustments of their fluxes are obvious, few studies have clearly illustrated these aspects. METHODS:  Trout red blood cells volume variations were estimated from water contents obtained by a gravimetric method. Intracellular K+ and Na+ contents, and Cl- content of haemolysed cells were determined by photometry and colorimetry, respectively. The taurine contribution to cell volume regulation was calculated from the net changes of water, K+, Cl- and Na+ contents. The intracellular pH was calculated from the chloride distribution across the cells membranes according to the Donnan equilibrium. RESULTS:  Cells responses to a rapid change (from 296 to 176 mOsm.kg-1)\u2028of the saline osmolality were examined in three conditions designed to not impact (Hypo. I)\u2028or to reduce the K+ (Hypo. II) and Cl- (Hypo. III) contributions to the volume regulation. Hypo. I condition caused an immediate increase in water content, followed by a 90 min. full regulation, concomitant with gradual lowering of K+ and Cl- contents and a surprising increase in Na+ content. Hypo. II and III conditions showed a partial and complete volume regulation, respectively. This was made possible by an increase in the taurine involvement. These experiments allowed to confirm that K+ and Cl- were released via KCl cotransport and by separate channels. The comparison of Hypo. I and III conditions led to the observation that the partially amiloride-sensitive Na+ influx is proportional to the taurine efflux; the latter being sustained mainly by a Na+\/taurine cotransport. The Hypo. II condition was suitable for the (Na+\/K+)ATPase activity inhibition. This effect could explain the observed lack of Na+ uptake, the consecutive depletion of intracellular taurine stock and the incomplete volume regulation. Finally, the results support the importance of taurine in pH control under Hypo. I (physiologic) condition. The alkalosis observed in Hypo. II and III conditions were the consequences of changes in the salines compositions, not of physiologic adjustments. CONCLUSION:  The regulatory volume decrease process of trout RBCs is complex and adjustable through coordinated osmolytes movements. The obliged decrease in K+ and\/or Cl- contributions stimulates taurine and Na+ pathways. This study highlights the importance of taurine as a compensatory variable in cell volume regulation and explains for the first time the significance of the Na+ uptake during this process<\/jats:p>","DOI":"10.33594\/000000440","type":"journal-article","created":{"date-parts":[[2021,10,19]],"date-time":"2021-10-19T20:59:29Z","timestamp":1634677169000},"page":"185-195","source":"Crossref","is-referenced-by-count":0,"title":["Evidence of Coordinated and Adjustable Osmolytes Movements Following Hyposmotic Swelling in Rainbow Trout Red Blood Cells"],"prefix":"10.33594","volume":"55","author":[{"name":"Institut de Recherche Dupuy De L\u00f4me, UMR CNRS 6027, Universit\u00e9 Bretagne Sud, C.E.R. Yves Coppens, Campus de Tohannic, 56017 Vannes Cedex (France)","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Val\u00e9rie","family":"Maxime","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"19235","published-online":{"date-parts":[[2021,10,20]]},"container-title":["Cellular Physiology and Biochemistry"],"original-title":[],"deposited":{"date-parts":[[2021,10,19]],"date-time":"2021-10-19T21:00:01Z","timestamp":1634677201000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cellphysiolbiochem.com\/Articles\/000440\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10,20]]},"references-count":0,"journal-issue":{"issue":"S1","published-online":{"date-parts":[[2021,10,20]]}},"URL":"https:\/\/doi.org\/10.33594\/000000440","relation":{},"ISSN":["1015-8987","1421-9778"],"issn-type":[{"type":"print","value":"1015-8987"},{"type":"electronic","value":"1421-9778"}],"subject":[],"published":{"date-parts":[[2021,10,20]]}}}