{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,26]],"date-time":"2026-04-26T05:14:49Z","timestamp":1777180489945,"version":"3.51.4"},"reference-count":0,"publisher":"Cell Physiol Biochem Press GmbH and Co KG","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Physiol Biochem"],"abstract":"<jats:p>Background\/Aims: Alpha synuclein (\u03b1SN) is a widely distributed protein in vertebrates whose physiological significance in many tissues remains unclear, being a key protein present in neurodegenerative disease such as Parkinson\u2019s Disease, Lewy Body Dementia, and in Sporadic-Inclusion Body Myositis. We search for \u03b1SN in skeletal muscle (SM) and neuronal plasma membrane isolated from brain (BR) from young and old rats. Methods: In isolated Sarcolemma from SM and from myelin-free neuronal plasma membrane isolated from BR, we determine by Western blot with anti-\u03b1SN (2B2D1) and anti-P-\u03b1SN (EP1536Y) the \u03b1SN membrane distribution, and the SM \u03b1SN intra and extracellular localization. Results: In SM and BR, \u03b1SN is present in cytosol (CYT) as monomer and oligomer structures mainly tetramers (TM) and in plasma membranes as oligomers (TM and PM). All \u03b1SN oligomers were localized in non-lipid rafts and their distribution was unaffected by cholesterol-depletion with Methyl-\u03b2-Cyclodextrin. Membranes with natively high cholesterol content such as Transverse Tubules in SM and myelin in BR, reduce the presence of \u03b1SN. Under the same experimental conditions, aged SM and BR plasma membranes show \u22482 folds more \u03b1SN. In SM, \u03b1SN is extruded without cell damage in young and old rats. Conclusion: We conclude that oligomeric \u03b1SN are regularly present in SM and BR plasma membranes of healthy young and old rats. Interestingly, low-cholesterol content membranes promote \u03b1SN interaction. SM, the largest tissue in vertebrate body is a source of \u03b1SN and may contribute to the presence of \u03b1SN in extracellular fluids.<\/jats:p>","DOI":"10.33594\/000000558","type":"journal-article","created":{"date-parts":[[2022,8,24]],"date-time":"2022-08-24T18:41:01Z","timestamp":1661366461000},"page":"382-400","source":"Crossref","is-referenced-by-count":6,"title":["Skeletal Muscle is a Source of \u03b1-Synuclein with a Sarcolemmal Non-Lipid Raft Distribution"],"prefix":"10.33594","volume":"56","author":[{"name":"Departamento de Bioqu\u00edmica, Facultad de Medicina, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Mexico City, M\u00e9xico","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ibrahim","family":"Ramirez-Soto","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alicia","family":"Ortega-Aguilar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"19235","published-online":{"date-parts":[[2022,8,25]]},"container-title":["Cellular Physiology and Biochemistry"],"original-title":[],"deposited":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T09:21:15Z","timestamp":1741771275000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cellphysiolbiochem.com\/Articles\/000558\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,25]]},"references-count":0,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2022,8,25]]}},"URL":"https:\/\/doi.org\/10.33594\/000000558","relation":{},"ISSN":["1015-8987","1421-9778"],"issn-type":[{"value":"1015-8987","type":"print"},{"value":"1421-9778","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,25]]}}}