{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:08:39Z","timestamp":1787026119363,"version":"build-2736575974"},"reference-count":0,"publisher":"Cell Physiol Biochem Press GmbH and Co KG","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Physiol Biochem"],"abstract":"<jats:p>Background\/Aims: Earlier studies have revealed the miRNAs and mRNAs involved in Polycystic Ovarian Syndrome (PCOS), but little is known about their regulatory networks. Methods: To address this issue, we applied a comprehensive miRNA, mRNA profiling approach in peripheral blood of PCOS patients. We identified 30 differential miRNAs and 3310 differential transcripts. A robust computational framework was created to integrate matched miRNA and mRNA expression profiles in PCOS using feed-forward loops. Results: The network consisted of differential miRNAs, transcription factors (TFs), and their common predicted target genes. The key network consisted of 14 non-orphan network clusters with 50 TF-gene pairs, 8 TF-TF pairs, 6 miRNA-TF pairs and 36 miRNA- gene pairs which were later dissected into 16 subclusters. Gene ontology annotations revealed that a host of signals (hormone, growth factors \u2013EGF\/PDGF, thrombopoietin, oxidative stress and vitamin\/nutrition) regulate MAPK signaling altering angiogenesis, JAK-STAT signaling, apoptosis, inflammatory and immune response and steroidogenesis in PCOS women. Conclusion: MAPK signaling is identified as the syndrome\u00b4s major dysregulated pathway. Our data imparts a robust foundation to expand the work and pave the way to focus efforts on p38MAPK targeted therapeutic strategies in PCOS.<\/jats:p>","DOI":"10.33594\/000000622","type":"journal-article","created":{"date-parts":[[2023,5,1]],"date-time":"2023-05-01T02:58:23Z","timestamp":1682909903000},"page":"137-156","source":"Crossref","is-referenced-by-count":8,"title":["miRNA-mRNA Network in PBMCs of PCOS Women Identifies Overactivated Stress-Activated Kinases"],"prefix":"10.33594","volume":"57","member":"19235","published-online":{"date-parts":[[2023,4,30]]},"container-title":["Cellular Physiology and Biochemistry"],"original-title":[],"deposited":{"date-parts":[[2023,5,1]],"date-time":"2023-05-01T02:58:25Z","timestamp":1682909905000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cellphysiolbiochem.com\/Articles\/000622\/index.html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,30]]},"references-count":0,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2023,4,30]]}},"URL":"https:\/\/doi.org\/10.33594\/000000622","relation":{},"ISSN":["1015-8987","1421-9778"],"issn-type":[{"value":"1015-8987","type":"print"},{"value":"1421-9778","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,30]]}}}