{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:08:38Z","timestamp":1787026118542,"version":"build-2736575974"},"reference-count":0,"publisher":"Cell Physiol Biochem Press GmbH and Co KG","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Physiol Biochem"],"abstract":"<jats:p>Background\/Aims: Immune cells are reported to upregulate CD47 during infection, however, the role of CD47 in innate and adaptive immune cells remains unclear. Methods: To bridge this knowledge gap, we analysed our single cell (sc)-RNA dataset along with other publicly available sc-RNA datasets from healthy controls, people with HIV-1 (PWH) and COVID-19 patients. We characterized each immune cell based on low, intermediate, and high expression of CD47. Results: Our analyses revealed that CD47high pDCs and monocytes exhibited relatively higher expression of IFN-\u03b1 regulatory genes, antiviral interferon-stimulated genes (ISGs) and MHC-I associated genes compared to CD47inter. and CD47low cells. Furthermore, CD47high NK and CD8+ T cells showed higher expression of antiviral ISGs, as well as genes encoding for cytotoxic markers like granzyme B, perforin, granulysin, interferon gamma and NKG7. Additionally, CD47high CD8+ T cells expressed higher levels of PD-1 and LAG-3 genes. Lastly, we found that CD47high B cells had enriched expression of genes involved in cell activation and humoral responses. Conclusion:Overall, our analyses revealed that innate and adaptive immune cells expressing elevated activation and functional gene signatures also express higher CD47 levels.<\/jats:p>","DOI":"10.33594\/000000715","type":"journal-article","created":{"date-parts":[[2024,7,28]],"date-time":"2024-07-28T14:09:51Z","timestamp":1722175791000},"page":"322-335","source":"Crossref","is-referenced-by-count":5,"title":["Single-Cell Analysis Reveals That CD47 mRNA Expression Correlates with Immune Cell Activation, Antiviral ISGs, and Cytotoxicity"],"prefix":"10.33594","volume":"58","member":"19235","published-online":{"date-parts":[[2024,7,26]]},"container-title":["Cellular Physiology and Biochemistry"],"original-title":[],"deposited":{"date-parts":[[2024,7,28]],"date-time":"2024-07-28T14:09:53Z","timestamp":1722175793000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cellphysiolbiochem.com\/Articles\/000715\/index.html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,26]]},"references-count":0,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2024,7,26]]}},"URL":"https:\/\/doi.org\/10.33594\/000000715","relation":{},"ISSN":["1015-8987","1421-9778"],"issn-type":[{"value":"1015-8987","type":"print"},{"value":"1421-9778","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,26]]}}}