{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T20:05:32Z","timestamp":1783627532616,"version":"3.55.0"},"reference-count":0,"publisher":"Cell Physiol Biochem Press GmbH and Co KG","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Physiol Biochem"],"abstract":"<jats:p>Background\/Aims: Aquaporin-3 (AQP3) is an aquaglyceroporin and peroxiporin that plays a crucial role in skin barrier homeostasis. Dysregulated AQP3 expression has been observed in different inflammatory skin conditions. Hidradenitis Suppurativa (HS) is an autoinflammatory keratinization disease that typically appears between 10 and 21 years of age, characterized by alteration of skin barrier homeostasis. Methods: To evaluate in vitro the role of AQP3 in the development of HS, we performed real-time PCR and Western blot to analyze gene and protein levels in human keratinocyte cell lines knock-out (KO) for NCSTN and PSENEN genes, simulating genetic-associated HS. Additionally, we investigated the impact of Glyceryl Glucoside (GG) on biological processes by performing MTT, scratch, proliferation assays and proteome studies. Results: We detected a significant decrease of the levels of AQP3 gene and protein in KO cell lines. GG effectively elevated the levels of mRNA and protein, significantly decreased the hyperproliferation rate, and enhanced cell migration in our in vitro model of genetic Hidradenitis Suppurativa. Pathway enrichment analysis further confirmed GG\u2019s role in the migration and proliferation pathways of keratinocytes. Conclusion: Our results suggest that AQP3 may act as a new novel actor in HS etio-pathogenesis, and GG could be further explored as potential treatment option for managing HS in patients.<\/jats:p>","DOI":"10.33594\/000000720","type":"journal-article","created":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T13:59:35Z","timestamp":1777471175000},"page":"404-417","source":"Crossref","is-referenced-by-count":1,"title":["Dysregulation of Aquaporin-3 and Glyceryl Glucoside Restoring Action in Hidradenitis Suppurativa in Vitro Models"],"prefix":"10.33594","volume":"58","author":[{"given":"Cecilia","family":"Del Vecchio","sequence":"first","affiliation":[{"name":"Department of Medical Surgical and Health Sciences, University of Trieste, Trieste, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rossella","family":"Gratton","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Scuola Internazionale Superiore di Studi Avanzati (SISSA), Trieste, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9cile","family":"Nait-Meddour","sequence":"additional","affiliation":[{"name":"INSERM, IMRB, Translational Neuropsychiatry, University Paris Est Cre\u0301teil, Cre\u0301teil, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena Maria","family":"Nardacchione","sequence":"additional","affiliation":[{"name":"Department of Medical Surgical and Health Sciences, University of Trieste, Trieste, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronald","family":"Moura","sequence":"additional","affiliation":[{"name":"Department of Advanced Diagnostics, Institute for Maternal and Child Health - IRCCS Burlo Garofolo, Trieste, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo","family":"Sommella","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, University of Salerno, Salerno, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara","family":"Moltrasio","sequence":"additional","affiliation":[{"name":"Dermatology Unit, Fondazione IRCCS Ca\u2019 Granda Ospedale Maggiore Policlinico, Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angelo Valerio","family":"Marzano","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology and Transplantation, Universit\u00e0 degli Studi di Milano, Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Blendi","family":"Ura","sequence":"additional","affiliation":[{"name":"Maternal-Neonatal Department, Institute for Maternal and Child Health - IRCCS Burlo Garofolo, Trieste, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donatella","family":"Mentino","sequence":"additional","affiliation":[{"name":"Department of Biosciences, Biotechnologies and Environment, University of Bari Aldo Moro, Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michele","family":"Boniotto","sequence":"additional","affiliation":[{"name":"INSERM, IMRB, Translational Neuropsychiatry, University Paris Est Cre\u0301teil, Cre\u0301teil, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adamo Pio","family":"d\u2019Adamo","sequence":"additional","affiliation":[{"name":"Department of Advanced Diagnostics, Institute for Maternal and Child Health - IRCCS Burlo Garofolo, Trieste, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Calamita","sequence":"additional","affiliation":[{"name":"Department of Biosciences, Biotechnologies and Environment, University of Bari Aldo Moro, Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio","family":"Crovella","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Research Center (LARC), Qatar University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola Maura","family":"Tricarico","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Institute for Maternal and Child Health - IRCCS Burlo Garofolo, Trieste, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"19235","published-online":{"date-parts":[[2024,7,30]]},"container-title":["Cellular Physiology and Biochemistry"],"original-title":[],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T13:59:36Z","timestamp":1777471176000},"score":1,"resource":{"primary":{"URL":"https:\/\/cellphysiolbiochem.com\/Articles\/000720\/index.html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,30]]},"references-count":0,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2024,7,30]]}},"URL":"https:\/\/doi.org\/10.33594\/000000720","relation":{},"ISSN":["1015-8987"],"issn-type":[{"value":"1015-8987","type":"print"}],"subject":[],"published":{"date-parts":[[2024,7,30]]}}}