{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,14]],"date-time":"2025-11-14T09:38:20Z","timestamp":1763113100393,"version":"3.45.0"},"reference-count":0,"publisher":"Cell Physiol Biochem Press GmbH and Co KG","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Physiol Biochem"],"abstract":"<jats:p>Background\/Aims: This study aimed to clarify the optimal amount of vitamin E required in the late stage of egg laying to explore the aging mechanism of the ovaries in this stage and the regulatory role of vitamin E in female reproductive aging using multiple experimental methods and multiomics joint analysis. Methods: The development of follicles was analyzed using Hematoxylin-eosin staining and terminal deoxynucleotidyl transferase deoxyuridine phosphate nick-end labeling staining. The content of follicle-stimulating hormone (FSH), luteinizing hormone (LH) and Estradiol (E2) was determined by Enzyme-linked Immunoassay Kit. The activities of superoxide dismutase (SOD) and malondialdehyde (MDA) in the serum using enzyme-linked immunosorbent assay kits. RNA-seq and Untargeted metabolomics analyses was used to to investigate the molecular mechanisms of vitamin E during ovarian aging. Results: The findings revealed that quail reproductive organs rapidly aged at the 11th egg-laying month. Adding vitamin E to the diet could significantly improve the egg production performance of quails. Furthermore, the vitamin E could promote the development of small yellow follicles (SYFs) by enhancing the antioxidant capacity and inhibiting cell apoptosis in quails. Moreover, we confirmed through RNA-seq and liquid chromatography with tandem mass spectrometry that a series of genes and metabolites could serve as biomarkers of vitamin E to improve ovarian aging. Conclusion: Altogether, these data demonstrated supplementing the diet with 250 mg\/kg vitamin E enhanced the body's energy metabolism, regulated SOD and MDA levels, enhanced antioxidant capacity, reduced cell apoptosis in preovulatory follicles, stimulated E2 secretion, promoted follicular development, and improved egg production performance.<\/jats:p>","DOI":"10.33594\/000000829","type":"journal-article","created":{"date-parts":[[2025,11,14]],"date-time":"2025-11-14T09:36:30Z","timestamp":1763112990000},"page":"811-825","source":"Crossref","is-referenced-by-count":0,"title":["Dietary Vitamin E Ameliorate Production Performance Via Pyruvate Metabolism Regulation in An Aged Laying Quails"],"prefix":"10.33594","volume":"59","author":[{"name":"College of Life Science and Agriculture Forestry, Qiqihar University, Qiqihar, China","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongyan","family":"Chen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mengqian","family":"Zou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tingting","family":"Cui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaotong","family":"Qin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fei","family":"Gao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shutong","family":"Deng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zheng","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qingying","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Branch of Animal Husbandry and Veterinary of Heilongjiang Academy of Agricultural Sciences, Qiqihar, China","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhigang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"19235","published-online":{"date-parts":[[2025,11,13]]},"container-title":["Cellular Physiology and Biochemistry"],"original-title":[],"deposited":{"date-parts":[[2025,11,14]],"date-time":"2025-11-14T09:36:31Z","timestamp":1763112991000},"score":1,"resource":{"primary":{"URL":"https:\/\/cellphysiolbiochem.com\/Articles\/000829\/index.html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,13]]},"references-count":0,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2025,11,13]]}},"URL":"https:\/\/doi.org\/10.33594\/000000829","relation":{},"ISSN":["1015-8987","1421-9778"],"issn-type":[{"value":"1015-8987","type":"print"},{"value":"1421-9778","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,13]]}}}