{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T08:43:07Z","timestamp":1776069787509,"version":"3.50.1"},"reference-count":0,"publisher":"Cell Physiol Biochem Press GmbH and Co KG","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Physiol Biochem"],"abstract":"<jats:p>Background\/Aims: Rapid and cost-effective quantification of triglycerides remains a challenge in industrial food quality control, where conventional analytical methods are often time-consuming and resource-intensive. This study aimed to develop and characterize an amperometric biosensor based on lipase (LPS) as a biorecognition element for efficient triglyceride detection in vegetable oils. Methods: Lipase was immobilized onto the electrode surface to catalyze the hydrolysis of glyceryl tributyrate, enabling electrochemical signal generation. Measurements were performed at an applied potential of \u2212600 mV, with a detection time of 7 seconds. The enzymatic reaction rate was derived from the slope of the amperometric signal (mV\/s), and calibration was conducted over a concentration range of 1\u20135 mM. Results: The biosensor demonstrated a strong linear response (R\u00b2 = 0.998) and high substrate affinity (KM = 0.13 mM). The system exhibited excellent repeatability and specificity, with minimal interference from other triglycerides. Long-term stability studies showed that the immobilized enzyme retained approximately 90% of its activity over 260 days and could be reused up to 27 times without significant performance loss. Conclusion: This biosensor represents a robust, rapid, and environmentally friendly analytical platform for triglyceride detection, offering a practical alternative to conventional techniques and strong potential for implementation in routine industrial quality control.<\/jats:p>","DOI":"10.33594\/000000860","type":"journal-article","created":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T07:45:57Z","timestamp":1776066357000},"page":"189-198","source":"Crossref","is-referenced-by-count":0,"title":["Characterization of a Biosensor For the Detection of Triglycerides in Vegetable Oils Using Lipase from Candida Rugosa"],"prefix":"10.33594","volume":"60","author":[{"given":"Danny Isa\u00edas Vera","family":"Guerrero","sequence":"first","affiliation":[{"name":"Carrera de Ingenieria Industrial. Facultad de Ciencia e Ingenieria. Universidad Estatal de Milagro"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Grether Luc\u00eda","family":"Real P\u00e9rez","sequence":"additional","affiliation":[{"name":"Dpto de Ingenier\u00eda Ambiental Agroalimentaria. Calidad y Seguridad y Salud en el Trabajo. Escuela Superior de Ingenier\u00eda y Tecnolog\u00eda. Universidad Internacional de la Rioja (UNIR), Logro\u00f1o, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank Guillermo","family":"Intriago Flor","sequence":"additional","affiliation":[{"name":"Departamento de Posgrado, Facultad de Posgrado, Universidad T\u00e9cnica de Manab\u00ed, Ecuador"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Patricio","family":"Mu\u00f1oz Murillo","sequence":"additional","affiliation":[{"name":"Departamento de Procesos Agroindustriales. Facultad de Agrociencias. Universidad T\u00e9cnica de Manab\u00ed"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miguel Andr\u00e9s","family":"Falcon\u00ed V\u00e9lez","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica, Facultad de Ciencias B\u00e1sicas. Universidad T\u00e9cnica de Manab\u00ed. Portoviejo, Ecuador, fUniversidad Agraria del Ecuador. Guayaquil, Ecuador"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmed","family":"El Salous","sequence":"additional","affiliation":[{"name":"Universidad Agraria del Ecuador. Guayaquil, Ecuador"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis Gerardo","family":"Merino Jaramillo","sequence":"additional","affiliation":[{"name":"Unidad Academica de Ciencias Agropecuarias. Universidad Cat\u00f3lica de Cuenca. Campus la Troncal. Ca\u00f1ar, Ecuador"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Denis Joaqu\u00edn","family":"Zambrano Ortiz","sequence":"additional","affiliation":[{"name":"Departamento de Ingenieria Industrial. Facultad de Ingenier\u00eda y Ciencias Aplicadas. Universidad T\u00e9cnica de Manab\u00ed, Portoviejo, Ecuador"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wagner Antonio","family":"Gorozabel Mu\u00f1oz","sequence":"additional","affiliation":[{"name":"Departamento de Procesos Agroindustriales. Facultad de Agrociencias. Universidad T\u00e9cnica de Manab\u00ed"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mauricio","family":"Valdiviezo Pe\u00f1a","sequence":"additional","affiliation":[{"name":"Departamento de Procesos Qu\u00edmicos, Alimentos y Biotecnolog\u00eda, Grupo de Investigaci\u00f3n: Seguridad Conservaci\u00f3n e Innovaci\u00f3n de Alimentos y Procesos. Facultad de Ingenier\u00eda y Ciencias Aplicadas. Universidad T\u00e9cnica de Manab\u00ed, Portoviejo, Ecuador"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Felipe Jad\u00e1n","family":"Piedra","sequence":"additional","affiliation":[{"name":"Departamento de Procesos Qu\u00edmicos, Alimentos y Biotecnolog\u00eda. Facultad de Ingenier\u00eda y Ciencias Aplicadas. Universidad T\u00e9cnica de Manab\u00ed, Portoviejo, Ecuador"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"19235","published-online":{"date-parts":[[2026,4,12]]},"container-title":["Cellular Physiology and Biochemistry"],"original-title":[],"deposited":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T07:45:57Z","timestamp":1776066357000},"score":1,"resource":{"primary":{"URL":"https:\/\/cellphysiolbiochem.com\/Articles\/000860\/index.html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,12]]},"references-count":0,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2026,4,12]]}},"URL":"https:\/\/doi.org\/10.33594\/000000860","relation":{},"ISSN":["1015-8987","1421-9778"],"issn-type":[{"value":"1015-8987","type":"print"},{"value":"1421-9778","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,12]]}}}