{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T10:21:04Z","timestamp":1787480464457,"version":"build-2736575974"},"reference-count":0,"publisher":"Cell Physiol Biochem Press GmbH and Co KG","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Physiol Biochem"],"abstract":"<jats:p>Background\/Aims: The aim of this research was to use Mebendazole as an anticancer candidate to reduce the dose of Flutamide and reduce its side effects. Method: In this in vitro study, we evaluated the effect of Mebendazole, Flutamide and Mebendazole-Flutamide combination therapy in LNCaP, DU145 and PC3 cell lines as representatives of human prostate cancer. The assessment includes scratch-wound assay, colony formation assay, flow cytometric analysis of apoptosis and DNA cell cycle, real-time PCR (BAX\/BCL2, E-cadherin, N-cadherin, Snail, HIF1\u03b1, VEGFC, KLK3, TUBB1 and TUBB3 genes). Results: To determine IC50 levels, cell lines were exposed to different concentration of the drugs. Our data indicated that IC50 values for Mebendazole (55\u03bcM) and for 3 cell lines and flutamide (12\u03bcM and 10\u03bcM) for PC3 and LNCaP\/DU145 respectively, with MTT were approved by flow cytometry in a dose and time-dependent manner which was as a consequence of cell cycle arrest at G1\/S phase. Furthermore, for the first time, we offered that combination of mebendazole and flutamide produced a greater inhibitory effect on cell viability, colony formation, and migration than either agent alone at the tested concentrations. The combination treatment also increased apoptotic cell populations and upregulated the BAX\/BCL2 mRNA ratio and E-cadherin expression in all three cell lines (P&lt;0.01) and downregulated the expression of TUBB1 in DU145 and TUBB3 genes in DU145 and PC3 cell lines (P&lt;0.01). Conclusion: Mebendazole in combination with flutamide reduced dose of flutamide and increased the sensitivity of prostate cancer cells to treatment. Therefore, this combination may represent a promising in vitro strategy that warrants further mechanistic and in vivo investigation.<\/jats:p>","DOI":"10.33594\/000000883","type":"journal-article","created":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T09:41:37Z","timestamp":1787478097000},"page":"458-472","source":"Crossref","is-referenced-by-count":0,"title":["Effects of Mebendazole and Flutamide Combination in Prostate Cancer Cell Lines: a Comprehensive in Vitro Analysis"],"prefix":"10.33594","volume":"60","author":[{"given":"Mohammad Reza Fattahi","family":"Fattahi","sequence":"first","affiliation":[{"name":"Students' Scientific Research Center, Tehran University of Medical Sciences, Tehran, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana","family":"Taheri","sequence":"additional","affiliation":[{"name":"Department of Pathology, Isfahan Kidney Disease Research Center, Isfahan University of Medical Sciences, Isfahan, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seyed Hassan","family":"Inanloo","sequence":"additional","affiliation":[{"name":"Department of Urology, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akram","family":"Mirzaei","sequence":"additional","affiliation":[{"name":"Urology Research Center, Tehran University of Medical Sciences, Tehran, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helia Azodian","family":"Ghajar","sequence":"additional","affiliation":[{"name":"Urology Research Center, Tehran University of Medical Sciences, Tehran, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonardo Oliveira","family":"Reis","sequence":"additional","affiliation":[{"name":"UroScience and Department of Surgery (Urology), School of Medical Sciences, University of Campinas, Unicamp, and Pontifical Catholic University of Campinas, PUC-Campinas, Campinas, Sao Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seyed Mohammad Kazem","family":"Aghamir","sequence":"additional","affiliation":[{"name":"Urology Research Center, Tehran University of Medical Sciences, Tehran, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"19235","published-online":{"date-parts":[[2026,8,23]]},"container-title":["Cellular Physiology and Biochemistry"],"original-title":[],"deposited":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T09:41:40Z","timestamp":1787478100000},"score":1,"resource":{"primary":{"URL":"https:\/\/cellphysiolbiochem.com\/Articles\/000883\/index.html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,23]]},"references-count":0,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2026,8,23]]}},"URL":"https:\/\/doi.org\/10.33594\/000000883","relation":{},"ISSN":["1015-8987","1421-9778"],"issn-type":[{"value":"1015-8987","type":"print"},{"value":"1421-9778","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,23]]}}}