{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T13:59:55Z","timestamp":1774619995768,"version":"3.50.1"},"reference-count":38,"publisher":"Elsevier BV","issue":"9","license":[{"start":{"date-parts":[[2016,9,1]],"date-time":"2016-09-01T00:00:00Z","timestamp":1472688000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2016,9,1]],"date-time":"2016-09-01T00:00:00Z","timestamp":1472688000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2019,9,28]],"date-time":"2019-09-28T00:00:00Z","timestamp":1569628800000},"content-version":"vor","delay-in-days":1122,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Poultry Science"],"published-print":{"date-parts":[[2016,9]]},"DOI":"10.3382\/ps\/pew147","type":"journal-article","created":{"date-parts":[[2016,4,27]],"date-time":"2016-04-27T01:10:01Z","timestamp":1461719401000},"page":"2023-2029","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":31,"title":["Development of a real-time quantitative RT-PCR to detect REV contamination in live vaccine"],"prefix":"10.1016","volume":"95","author":[{"given":"Huaibiao","family":"Luan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yixin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhizhong","family":"Cui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuang","family":"Chang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peng","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.3382\/ps\/pew147_bib1","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1080\/03079459308418942","article-title":"Detection of reticuloendotheliosis virus infection using the polymerase chain reaction","volume":"22","author":"Aly","year":"1993","journal-title":"Avian Pathol"},{"key":"10.3382\/ps\/pew147_bib2","doi-asserted-by":"crossref","first-page":"2389","DOI":"10.3382\/ps.2010-00899","article-title":"Detection of reticuloendotheliosis virus as a contaminant of fowl pox vaccines","volume":"89","author":"Awad","year":"2010","journal-title":"Poult. Sci"},{"key":"10.3382\/ps\/pew147_bib3","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1111\/j.1751-0813.1979.tb15261.x","article-title":"Infection studies on a reticuloendotheliosis virus contaminant of a commercial Marek's disease vaccine","volume":"55","author":"Bagust","year":"1979","journal-title":"Aust. Vet. J"},{"key":"10.3382\/ps\/pew147_bib4","first-page":"147","article-title":"Detection of fowl poxvirus integrated with reticuloendotheliosis virus sequences from an outbreak in backyard chickens in India","volume":"47","author":"Biswas","year":"2011","journal-title":"Vet. Ital."},{"key":"10.3382\/ps\/pew147_bib5","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1080\/03079450903349188","article-title":"Simultaneous endemic infections with subgroup J avian leukosis virus and reticuloendotheliosis virus in commercial and local breeds of chickens","volume":"38","author":"Cui","year":"2009","journal-title":"Avian Pathol"},{"key":"10.3382\/ps\/pew147_bib6","doi-asserted-by":"crossref","first-page":"1295","DOI":"10.1292\/jvms.69.1295","article-title":"Occurrence of reticuloendotheliosis in Chinese partridge","volume":"69","author":"Cheng","year":"2007","journal-title":"J. Vet. Med. Sci"},{"key":"10.3382\/ps\/pew147_bib7","doi-asserted-by":"crossref","first-page":"4237","DOI":"10.4049\/jimmunol.136.11.4237","article-title":"Monoclonal antibodies against avian reticuloendotheliosis virus: Identification of strain-specific and strain-common epitopes","volume":"136","author":"Cui","year":"1986","journal-title":"J. Immunol."},{"key":"10.3382\/ps\/pew147_bib8","doi-asserted-by":"crossref","first-page":"32","DOI":"10.2307\/1590945","article-title":"Monoclonal-antibody-mediated enzyme-linked immunosorbent assay for detection of reticuloendotheliosis viruses","volume":"32","author":"Cui","year":"1988","journal-title":"Avian Dis"},{"key":"10.3382\/ps\/pew147_bib9","doi-asserted-by":"crossref","first-page":"102","DOI":"10.2307\/1593017","article-title":"In vivo events of retroiral long terminal repeat integration into Marek's disease virus in commerial poultry: Detection of chimeric molecules as a marker","volume":"45","author":"Davidson","year":"2001","journal-title":"Avian Dis"},{"key":"10.3382\/ps\/pew147_bib10","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1080\/03079459808419275","article-title":"Field isolates of fowlpox virus contaminated with reticuloendotheliosis virus","volume":"27","author":"Diallo","year":"1998","journal-title":"Avian pathol"},{"key":"10.3382\/ps\/pew147_bib11","first-page":"588","article-title":"Study on the natural recombinant fowlpox virus containing genomic fragment from REV","volume":"44","author":"Ding","year":"2004","journal-title":"Acta Microbiologica Sinica"},{"key":"10.3382\/ps\/pew147_bib12","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1080\/03079459608419118","article-title":"An outbreak of lymphomas in commercial broiler breeder chickens vaccinated with a fowlpox vaccine contaminated with reticuloendotheliosis virus","volume":"25","author":"Fadly","year":"1996","journal-title":"Avian Pathol"},{"key":"10.3382\/ps\/pew147_bib13","first-page":"301","article-title":"Detection of reticuloendotheliosis virus in live virus vaccines of poultry","volume":"126","author":"Fadly","year":"2006","journal-title":"Dev Biol"},{"key":"10.3382\/ps\/pew147_bib14","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1637\/0005-2086(2003)047[0343:MCORVI]2.0.CO;2","article-title":"Molecular characterization of reticuloendotheliosis virus insertions in the genome of field and vaccine strains of fowl poxvirus","volume":"47","author":"Garcia","year":"2003","journal-title":"Avian Dis"},{"key":"10.3382\/ps\/pew147_bib15","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1006\/viro.1997.8691","article-title":"Field and vaccine strains of fowlpox virus carry integrated sequences from the avian retrovirus, reticuloendotheliosis virus","volume":"235","author":"Hertig","year":"1997","journal-title":"Virology"},{"key":"10.3382\/ps\/pew147_bib16","doi-asserted-by":"crossref","first-page":"991","DOI":"10.1073\/pnas.89.3.991","article-title":"Retrovirus insertion into herpesvirus in vitro and in vivo","volume":"89","author":"Isfort","year":"1992","journal-title":"Proc. Natl. Acad. Sci. U S A"},{"key":"10.3382\/ps\/pew147_bib17","doi-asserted-by":"crossref","first-page":"3855","DOI":"10.1073\/pnas.90.9.3855","article-title":"Retroviral insertions into a herpesvirus are clustered at the junctions of the short repeat and short unique sequences","volume":"90","author":"Jones","year":"1993","journal-title":"Proc. Natl. Acad. Sci."},{"key":"10.3382\/ps\/pew147_bib18","doi-asserted-by":"crossref","first-page":"2460","DOI":"10.1128\/jvi.70.4.2460-2467.1996","article-title":"Retroviral insertional activation in a herpesvirus: Transcriptional activation of US genes by an integrated long terminal repeat in a Marek's disease virus clone","volume":"70","author":"Jones","year":"1996","journal-title":"J. Virol."},{"key":"10.3382\/ps\/pew147_bib19","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1006\/viro.1993.1018","article-title":"Retrovirus insertion into herpesvirus: Characterization of a Marek's disease virus harboring a solo LTR","volume":"192","author":"Kost","year":"1993","journal-title":"Virology."},{"key":"10.3382\/ps\/pew147_bib20","doi-asserted-by":"crossref","first-page":"643","DOI":"10.3382\/ps\/pev034","article-title":"Isolation, identification, and whole genome sequencing of reticuloendotheliosis virus from a vaccine against Marek's disease","volume":"94","author":"Li","year":"2015","journal-title":"Poult. Sci"},{"key":"10.3382\/ps\/pew147_bib21","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1016\/j.jviromet.2011.12.001","article-title":"Development of TaqMan real-time PCR assay for detection and quantitation of reticuloendotheliosis virus","volume":"179","author":"Li","year":"2012","journal-title":"J. Virol. Methods"},{"key":"10.3382\/ps\/pew147_bib22","first-page":"1","article-title":"Development and preliminary application of a SYBR GREEN I based multiple real-time PCR assay for detection of reticuloendotheliosis virus in chicken embryo fibrolasts","volume":"23","author":"Miao","year":"2015","journal-title":"Chin. J. Anim. Infect. Dis."},{"key":"10.3382\/ps\/pew147_bib23","doi-asserted-by":"crossref","first-page":"827","DOI":"10.2307\/1593055","article-title":"Reticuloendotheliosis virus (REV) long terminal repeats incorporated in the genomes of commercial fowl poxvirus vaccines and pigeon poxviruses without indication of the presence of infectious REV","volume":"44","author":"Moore","year":"2000","journal-title":"Avian Dis"},{"key":"10.3382\/ps\/pew147_bib24","doi-asserted-by":"crossref","first-page":"5855","DOI":"10.1128\/JVI.77.10.5855-5862.2003","article-title":"Reticuloendotheliosis virus sequences within the genomes of field strains of fowlpox virus display variability","volume":"77","author":"Pratik","year":"2003","journal-title":"J. Virol"},{"key":"10.3382\/ps\/pew147_bib25","first-page":"75","article-title":"Use of the polymerase chain reaction for the detection of reticuloendotheliosis virus in Marek's disease vaccines and chicken tissues","volume":"43","author":"Reimann","year":"1996","journal-title":"Zentralbl Veterinarmed B"},{"key":"10.3382\/ps\/pew147_bib26","doi-asserted-by":"crossref","first-page":"5855","DOI":"10.1128\/JVI.77.10.5855-5862.2003","article-title":"Reticuloendotheliosis virus sequences within the genomes of field strains of fowlpox virus display variability","volume":"77","author":"Singh","year":"2003","journal-title":"J. Virol"},{"key":"10.3382\/ps\/pew147_bib27","doi-asserted-by":"crossref","first-page":"596","DOI":"10.1016\/j.vaccine.2014.12.057","article-title":"A recombinant field strain of Marek's disease (MD) virus with reticuloendotheliosis virus long terminal repeat insert lacking the meq gene as a vaccine against MD","volume":"33","author":"Su","year":"2015","journal-title":"Vaccine"},{"key":"10.3382\/ps\/pew147_bib28","doi-asserted-by":"crossref","first-page":"2641","DOI":"10.1007\/s11434-009-0364-3","article-title":"A BAC clone of MDV strain GX0101 with REV-LTR integration retained its pathogenicity","volume":"54","author":"Sun","year":"2009","journal-title":"Chinese Science Bulletin."},{"key":"10.3382\/ps\/pew147_bib29","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1016\/j.virol.2009.11.017","article-title":"Functional evaluation of the role of reticuloendotheliosis virus long terminal repeat (LTR) integrated into the genome of a field strain of Marek's disease virus","volume":"397","author":"Sun","year":"2010","journal-title":"Virology"},{"key":"10.3382\/ps\/pew147_bib30","first-page":"35","article-title":"Isolation of Reticuloendotheliosis Virus from a fowlpox live vaccine and env gene sequence analysis","volume":"18","author":"Wang","year":"2010","journal-title":"Chin. J. Anim. Infect. Dis."},{"key":"10.3382\/ps\/pew147_bib31","first-page":"340","article-title":"Sequencing and analysis of whole genome nucleotide sequence of Chinese REV isolate HA9901","volume":"35","author":"Wang","year":"2005","journal-title":"SCI China Ser. C"},{"key":"10.3382\/ps\/pew147_bib32","doi-asserted-by":"crossref","first-page":"e43422","DOI":"10.1371\/journal.pone.0043422","article-title":"Probable congenital transmission of reticuloendotheliosis virus caused by vaccination with contaminated vaccines","volume":"7","author":"Wei","year":"2012","journal-title":"PLoS One"},{"key":"10.3382\/ps\/pew147_bib33","doi-asserted-by":"crossref","first-page":"226","DOI":"10.2307\/1590836","article-title":"Vertical transmission of reticuloendotheliosis virus in breeder turkeys","volume":"33","author":"Witter","year":"1989","journal-title":"Avian Dis"},{"key":"10.3382\/ps\/pew147_bib34","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1128\/iai.26.1.90-98.1979","article-title":"Depression of vaccinal immunity to Marek's disease by infection with reticuloendotheliosis virus","volume":"26","author":"Witter","year":"1979","journal-title":"Infect. Immun"},{"key":"10.3382\/ps\/pew147_bib35","first-page":"141","article-title":"Isolation of a reticuloendotheliosis virus from chickens inoculated with Marek's disease vaccine","volume":"16","author":"Yuasa","year":"1976","journal-title":"Natl. Inst. Anim. Health Q"},{"key":"10.3382\/ps\/pew147_bib36","first-page":"39","article-title":"Coinfection of reticuloendotheliosis virus and marek's disease virus in broiler flock","volume":"27","author":"Zhang","year":"2005","journal-title":"Chin. J. Pre. Vet. Med."},{"key":"10.3382\/ps\/pew147_bib37","first-page":"621","article-title":"Serological survey of the Reticuloendotheliosis virus infection in China native chicken flocks","volume":"32","author":"Zhao","year":"2012","journal-title":"Pak. Vet. J"},{"key":"10.3382\/ps\/pew147_bib38","first-page":"795","article-title":"Research of detection method of REV using real-time PCR","volume":"27","author":"Zhao","year":"2007","journal-title":"Chin. J. Vet. Sci"}],"container-title":["Poultry Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0032579119317225?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0032579119317225?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/academic.oup.com\/ps\/article-pdf\/95\/9\/2023\/7995091\/pew147.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,21]],"date-time":"2025-09-21T20:44:33Z","timestamp":1758487473000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0032579119317225"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,9]]},"references-count":38,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2016,9]]}},"alternative-id":["S0032579119317225"],"URL":"https:\/\/doi.org\/10.3382\/ps\/pew147","relation":{},"ISSN":["0032-5791"],"issn-type":[{"value":"0032-5791","type":"print"}],"subject":[],"published":{"date-parts":[[2016,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development of a real-time quantitative RT-PCR to detect REV contamination in live vaccine","name":"articletitle","label":"Article Title"},{"value":"Poultry Science","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.3382\/ps\/pew147","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2016 Poultry Science Association Inc. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}