{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T14:50:25Z","timestamp":1788965425070,"version":"build-2803163510"},"reference-count":31,"publisher":"Elsevier BV","issue":"7","license":[{"start":{"date-parts":[[2017,7,1]],"date-time":"2017-07-01T00:00:00Z","timestamp":1498867200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2017,7,1]],"date-time":"2017-07-01T00:00:00Z","timestamp":1498867200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2019,9,27]],"date-time":"2019-09-27T00:00:00Z","timestamp":1569542400000},"content-version":"vor","delay-in-days":818,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Poultry Science"],"published-print":{"date-parts":[[2017,7]]},"DOI":"10.3382\/ps\/pex034","type":"journal-article","created":{"date-parts":[[2017,3,3]],"date-time":"2017-03-03T03:45:06Z","timestamp":1488512706000},"page":"2471-2479","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"title":["Phenamil, an amiloride derivative, restricts long bone growth and alters keeled-sternum bone architecture in growing chickens"],"prefix":"10.1016","volume":"96","author":[{"given":"Tara R.","family":"Price","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristin","family":"Moncada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hector","family":"Leyva-Jimenez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kye Won","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Tontonoz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosemary L.","family":"Walzem","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.3382\/ps\/pex034_bib1","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1590\/S1516-635X2006000200001","article-title":"Bone mineral density: review","volume":"8","author":"Almeida Paz","year":"2006","journal-title":"Braz. J. Poult. Sci."},{"key":"10.3382\/ps\/pex034_bib2","doi-asserted-by":"crossref","first-page":"1289","DOI":"10.1093\/ps\/81.9.1289","article-title":"Growth of the femur and tibia of a commercial broiler line","volume":"81","author":"Applegate","year":"2002","journal-title":"Poult. Sci."},{"key":"10.3382\/ps\/pex034_bib3","doi-asserted-by":"crossref","first-page":"77S","DOI":"10.1016\/8756-3282(95)00183-E","article-title":"Osteoblast and chondroblast differentiation","volume":"17","author":"Aubin","year":"1995","journal-title":"Bone"},{"key":"10.3382\/ps\/pex034_bib4","first-page":"48","article-title":"Mechanisms involved in the vascular actions of phenamil","volume":"27","author":"Beschea-Chiriac","year":"2001","journal-title":"Ann. Univ. Med. Vet. Agron. Iasi"},{"key":"10.3382\/ps\/pex034_bib5","doi-asserted-by":"crossref","first-page":"913","DOI":"10.1002\/dvdy.24425","article-title":"Pannexin 3 is required for late stage bone growth but not for initiation of ossification in avian embryos","volume":"245","author":"Bond","year":"2016","journal-title":"Devel. Dynam."},{"key":"10.3382\/ps\/pex034_bib6","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1080\/08977190412331279890","article-title":"Bone morphogenetic proteins","volume":"22","author":"Chen","year":"2004","journal-title":"Growth Factors"},{"key":"10.3382\/ps\/pex034_bib7","series-title":"Osteoimmunology: An Introduction","author":"Choi","year":"2010"},{"key":"10.3382\/ps\/pex034_bib8","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1007\/BF01870698","article-title":"Phenamil: an irreversible inhibitor of sodium channels in the toad urinary bladder","volume":"87","author":"Garvin","year":"1985","journal-title":"J. Membr. Biol."},{"key":"10.3382\/ps\/pex034_bib9","doi-asserted-by":"crossref","first-page":"162","DOI":"10.3382\/ps\/peu052","article-title":"Genetic analysis of leg problems and growth in a random mating broiler population","volume":"94","author":"Gonz\u00e1lez-Cer\u00f3n","year":"2015","journal-title":"Poult. Sci."},{"key":"10.3382\/ps\/pex034_bib10","doi-asserted-by":"crossref","first-page":"1103","DOI":"10.1016\/j.bone.2006.11.027","article-title":"PINP: A serum biomarker of bone formation in the rat","volume":"40","author":"Hale","year":"2007","journal-title":"Bone"},{"key":"10.3382\/ps\/pex034_bib11","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1038\/nature01660","article-title":"Control of osteoblast function and regulation of bone mass","volume":"423","author":"Harada","year":"2003","journal-title":"Nature"},{"key":"10.3382\/ps\/pex034_bib12","doi-asserted-by":"crossref","first-page":"969","DOI":"10.1080\/00103628009367096","article-title":"A nitric acid plant tissue digest method for use with inductively coupled plasma spectrometry","volume":"11","author":"Havlin","year":"2008","journal-title":"Comm. Soil. Sci. Plan."},{"key":"10.3382\/ps\/pex034_bib13","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1093\/jaoac\/58.3.436","article-title":"Collaborative study of wet and dry ashing techniques for the elemental analysis of plant tissue by atomic absorption spectrophotometry","volume":"58","author":"Isaac","year":"1975","journal-title":"J. AOAC."},{"key":"10.3382\/ps\/pex034_bib14","doi-asserted-by":"crossref","first-page":"1951","DOI":"10.3382\/ps.2012-02909","article-title":"Development of an enzyme-linked immunosorbent assay for detection of chicken osteocalcin and its use in evaluation of perch effects on bone remodeling in caged White Leghorns","volume":"92","author":"Jiang","year":"2013","journal-title":"Poult. Sci."},{"key":"10.3382\/ps\/pex034_bib15","doi-asserted-by":"crossref","first-page":"e1005250","DOI":"10.1371\/journal.pgen.1005250","article-title":"Genetic regulation of bone metabolism in the chicken: similarities and differences to Mammalian systems","volume":"11","author":"Johnsson","year":"2015","journal-title":"PLoS Gene"},{"key":"10.3382\/ps\/pex034_bib16","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1080\/03079450020016751","article-title":"Physiological, management and environmental triggers of the ascites syndrome: a review","volume":"29","author":"Julian","year":"2000","journal-title":"Avian Pathol."},{"key":"10.3382\/ps\/pex034_bib17","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/S8756-3282(97)00279-2","article-title":"Collagen cross-links in mineralizing tissues: A review of their chemistry, function, and clinical relevance","volume":"22","author":"Knott","year":"1998","journal-title":"Bone"},{"key":"10.3382\/ps\/pex034_bib18","doi-asserted-by":"crossref","first-page":"2053","DOI":"10.1093\/ps\/86.10.2053","article-title":"Tibial dyschondroplasia 40 years later","volume":"86","author":"Leach","year":"2007","journal-title":"Poult. Sci."},{"key":"10.3382\/ps\/pex034_bib19","doi-asserted-by":"crossref","first-page":"897","DOI":"10.3382\/ps.0730897","article-title":"Skeletal growth of commercial poultry species","volume":"73","author":"Lilburn","year":"1994","journal-title":"Poult. Sci."},{"key":"10.3382\/ps\/pex034_bib20","doi-asserted-by":"crossref","first-page":"1787","DOI":"10.1093\/ps\/82.11.1787","article-title":"Bone densitometry as an indicator of percentage tibia ash in broiler chicks fed varying dietary calcium and phosphorus levels","volume":"82","author":"Onyango","year":"2003","journal-title":"Poult. Sci."},{"key":"10.3382\/ps\/pex034_bib21","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/0012-1606(81)90324-9","article-title":"Characterization of a bone-specific alkaline phosphatase in chick limb mesenchymal cell cultures","volume":"86","author":"Osdoby","year":"1981","journal-title":"Dev. Biol."},{"key":"10.3382\/ps\/pex034_bib22","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/0012-1606(81)90325-0","article-title":"First bone formation in the developing chick limb","volume":"86","author":"Osdoby","year":"1981","journal-title":"Dev. Biol."},{"key":"10.3382\/ps\/pex034_bib23","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1161\/01.ATV.17.4.680","article-title":"Lipid oxidation products have opposite effects on calcifying vascular cell and bone cell differentiation. A possible explanation for the paradox of arterial calcification in osteoporotic patients","volume":"17","author":"Parhami","year":"1997","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"10.3382\/ps\/pex034_bib24","doi-asserted-by":"crossref","first-page":"3905","DOI":"10.1128\/MCB.00002-09","article-title":"The small molecule phenamil induces osteoblast differentiation and mineralization","volume":"29","author":"Park","year":"2009","journal-title":"Mol. Cell. Biol."},{"key":"10.3382\/ps\/pex034_bib25","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1111\/j.1440-1797.1998.tb00369.x","article-title":"Osteocalcin as a biochemical marker of bone turnover","volume":"4","author":"Polak-Jonkisz","year":"1998","journal-title":"Nephrol"},{"key":"10.3382\/ps\/pex034_bib26","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/nmeth.2089","article-title":"NIH image to image J: 25 years of image analysis","volume":"9","author":"Rasband","year":"2012","journal-title":"Nat. Meth."},{"key":"10.3382\/ps\/pex034_bib27","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1016\/0012-1606(85)90319-7","article-title":"Developmental acquisition of type X collagen in the embryonic chick tibiotarsus","volume":"107","author":"Schmid","year":"1985","journal-title":"Dev. Biol."},{"key":"10.3382\/ps\/pex034_bib28","doi-asserted-by":"crossref","first-page":"62","DOI":"10.3382\/ps.2011-01599","article-title":"The effects of broiler chicken growth rate on valgus, varus, and tibial dyschondroplasia","volume":"91","author":"Shim","year":"2012","journal-title":"Poult. Sci."},{"key":"10.3382\/ps\/pex034_bib29","doi-asserted-by":"crossref","first-page":"1790","DOI":"10.3382\/ps.2011-01968","article-title":"The effects of growth rate on leg morphology and tibia breaking strength, mineral density, mineral content, and bone ash in broilers","volume":"91","author":"Shim","year":"2012","journal-title":"Poult. Sci"},{"key":"10.3382\/ps\/pex034_bib30","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1359\/jbmr.071025","article-title":"Murine and chicken chondrocytes regulate osteoclastogenesis by producing RANKL in response to BMP2","volume":"23","author":"Usui","year":"2008","journal-title":"J. Bone Miner. Res."},{"key":"10.3382\/ps\/pex034_bib31","first-page":"122","article-title":"Causes and prevention of bone fractures","volume":"19","author":"Whitehead","year":"2007","journal-title":"Austr. Poult. Sci. Symp"}],"container-title":["Poultry Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0032579119314427?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0032579119314427?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/academic.oup.com\/ps\/article-pdf\/96\/7\/2471\/24194516\/pex034.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,21]],"date-time":"2025-09-21T20:43:51Z","timestamp":1758487431000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0032579119314427"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,7]]},"references-count":31,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2017,7]]}},"alternative-id":["S0032579119314427"],"URL":"https:\/\/doi.org\/10.3382\/ps\/pex034","relation":{},"ISSN":["0032-5791"],"issn-type":[{"value":"0032-5791","type":"print"}],"subject":[],"published":{"date-parts":[[2017,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Phenamil, an amiloride derivative, restricts long bone growth and alters keeled-sternum bone architecture in growing chickens","name":"articletitle","label":"Article Title"},{"value":"Poultry Science","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.3382\/ps\/pex034","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2017 Poultry Science Association Inc. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}