{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T01:45:49Z","timestamp":1790646349517,"version":"4.1.0"},"reference-count":42,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2019,12,30]],"date-time":"2019-12-30T00:00:00Z","timestamp":1577664000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"Mediterranean Agronomic Institute of Zaragoza"},{"name":"IAMZ"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Poultry Science"],"published-print":{"date-parts":[[2020,1]]},"DOI":"10.3382\/ps\/pez466","type":"journal-article","created":{"date-parts":[[2019,8,9]],"date-time":"2019-08-09T20:45:04Z","timestamp":1565383504000},"page":"235-245","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":22,"title":["Evaluation of dietary supplementation of a novel microbial muramidase on gastrointestinal functionality and growth performance in broiler chickens"],"prefix":"10.1016","volume":"99","author":[{"given":"Mounira","family":"Sais","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana C.","family":"Barroeta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"L\u00f3pez-Colom","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miquel","family":"Nofrar\u00edas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nat\u00e0lia","family":"Maj\u00f3","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rual","family":"Lopez-Ulibarri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Estefan\u00eda","family":"P\u00e9rez Calvo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susana M.","family":"Mart\u00edn-Or\u00fae","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.3382\/ps\/pez466_bib1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0185153","article-title":"Exogenous dietary lysozyme improves the growth performance and gut microbiota in broiler chickens targeting the antioxidant and non-specific immunity mRNA expression","volume":"12","author":"Abdel-Latif","year":"2017","journal-title":"PLoS One"},{"key":"10.3382\/ps\/pez466_bib2","doi-asserted-by":"crossref","first-page":"4084","DOI":"10.1128\/AEM.64.10.4084-4088.1998","article-title":"Effective recovery of bacterial DNA and percent-guanine-plus-cytosine-based analysis of community structure in the gastrointestinal tract of broiler chickens","volume":"64","author":"Apajalahti","year":"1998","journal-title":"Appl. Environ. Microbiol."},{"key":"10.3382\/ps\/pez466_bib3","doi-asserted-by":"crossref","first-page":"2","DOI":"10.3389\/fvets.2016.00002","article-title":"Development of the chick microbiome: how early exposure influences future microbial diversity","volume":"3","author":"Ballou","year":"2016","journal-title":"Front. Vet. Sci."},{"key":"10.3382\/ps\/pez466_bib4","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1038\/nmeth.2276","article-title":"Quality-filtering vastly improves diversity estimates from Illumina amplicon sequencing","volume":"10","author":"Bokulich","year":"2013","journal-title":"Nat. Methods"},{"key":"10.3382\/ps\/pez466_bib5","doi-asserted-by":"crossref","first-page":"921","DOI":"10.1093\/jn\/138.5.921","article-title":"Lysozyme transgenic goats' milk influences gastrointestinal morphology in young pigs","volume":"138","author":"Brundige","year":"2008","journal-title":"J. Nutr."},{"key":"10.3382\/ps\/pez466_bib6","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.anifeedsci.2017.09.012","article-title":"Gastrointestinal functionality in animal nutrition and health: New opportunities for sustainable animal production","volume":"234","author":"Celi","year":"2017","journal-title":"Anim. Feed Sci. Technol."},{"key":"10.3382\/ps\/pez466_bib7","doi-asserted-by":"crossref","first-page":"1155","DOI":"10.1093\/ps\/83.7.1155","article-title":"Fatty acid content in chicken thigh and breast as affected by dietary polyunsaturation level","volume":"83","author":"Cortinas","year":"2004","journal-title":"Poult. Sci."},{"key":"10.3382\/ps\/pez466_bib8","doi-asserted-by":"crossref","first-page":"2755","DOI":"10.1128\/IAI.01375-10","article-title":"Immune response of chicken gut to natural colonization by gut microflora and to Salmonella enterica serovar Enteritidis infection","volume":"79","author":"Crhanova","year":"2011","journal-title":"Infect. Immun"},{"key":"10.3382\/ps\/pez466_bib9","doi-asserted-by":"crossref","first-page":"482","DOI":"10.3390\/ijms17040482","article-title":"Gastrointestinal endogenous protein-derived bioactive peptides: an in vitro study of their gut modulatory potential","volume":"17","author":"Dave","year":"2016","journal-title":"Int. J. Mol. Sci."},{"key":"10.3382\/ps\/pez466_bib10","doi-asserted-by":"crossref","first-page":"1131S","DOI":"10.1093\/ajcn\/73.6.1131S","article-title":"Microbial modulation of innate defense: goblet cells and the intestinal mucus layer","volume":"73","author":"Deplancke","year":"2001","journal-title":"J. Clin. Nutr."},{"key":"10.3382\/ps\/pez466_bib12","series-title":"Necesidades nutricionales para avicultura: pollos de carne y aves de puesta","author":"Fundaci\u00f3n Espa\u00f1ola para el Desarrollo de la Nutrici\u00f3n Animal (FEDNA)","year":"2008"},{"key":"10.3382\/ps\/pez466_bib13","doi-asserted-by":"crossref","first-page":"309","DOI":"10.20870\/productions-animales.2005.18.5.3535","article-title":"La microflore digestive des volailles: facteurs de variation et cons\u00e9quences pour l'animal","volume":"18","author":"Gabriel","year":"2005","journal-title":"INRA Prod. Anim."},{"key":"10.3382\/ps\/pez466_bib14","first-page":"1","article-title":"The effect of dietary lysozyme with EDTA on growth performance and intestinal microbiota of broiler chickens in each period of the growth cycle","volume":"26","author":"Gong","year":"2016","journal-title":"J. Appl. Poult."},{"key":"10.3382\/ps\/pez466_bib15","doi-asserted-by":"crossref","first-page":"2080","DOI":"10.3382\/ps\/pey556","article-title":"Evaluation of a microbial muramidase supplementation on growth performance, apparent ileal digestibility, and intestinal histology of broiler chickens","volume":"98","author":"Goodarzi Boroojeni","year":"2019","journal-title":"Poult. Sci."},{"key":"10.3382\/ps\/pez466_bib16","doi-asserted-by":"crossref","first-page":"75","DOI":"10.2174\/187153007780832091","article-title":"The role and potential therapeutical applications of antimicrobial proteins in infectious and inflammatory diseases","volume":"7","author":"Harder","year":"2007","journal-title":"Endocr. Metab. Immune. Disord. Drug Targets"},{"key":"10.3382\/ps\/pez466_bib17","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1159\/000181181","article-title":"Bacterial peptidoglycan-degrading enzymes and their impact on host muropeptide detection","volume":"1","author":"Humann","year":"2009","journal-title":"J. Innate Immun."},{"key":"10.3382\/ps\/pez466_bib18","doi-asserted-by":"crossref","first-page":"1214","DOI":"10.1093\/jn\/132.6.1214","article-title":"Rice expressing lactoferrin and lysozyme has antibiotic-like properties when fed to chicks","volume":"132","author":"Humphrey","year":"2002","journal-title":"J. Nutr."},{"key":"10.3382\/ps\/pez466_bib19","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1111\/j.1749-6632.2012.06813.x","article-title":"Bacterial cell-wall recycling","volume":"1277","author":"Johnson","year":"2013","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"10.3382\/ps\/pez466_bib20","doi-asserted-by":"crossref","first-page":"565","DOI":"10.3382\/ps.2011-01934","article-title":"Genetic parameters of foot-pad dermatitis and body weight in purebred broiler lines in 2 contrasting environments","volume":"91","author":"Kapell","year":"2012","journal-title":"Poult. Sci."},{"key":"10.3382\/ps\/pez466_bib21","doi-asserted-by":"crossref","first-page":"1745","DOI":"10.3382\/ps.2013-03739","article-title":"The effect of protease, amylase, and nonstarch polysaccharide-degrading enzyme supplementation on nutrient utilization and growth performance of broiler chickens fed corn-soybean meal-based diets","volume":"93","author":"Kaczmarek","year":"2014","journal-title":"Poult. Sci."},{"key":"10.3382\/ps\/pez466_bib22","doi-asserted-by":"crossref","first-page":"235","DOI":"10.3389\/fmicb.2018.00235","article-title":"Host and environmental factors affecting the intestinal microbiota in chickens","volume":"16","author":"Kers","year":"2018","journal-title":"Front. Microbiol."},{"key":"10.3382\/ps\/pez466_bib23","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1051\/animres:19820408","article-title":"The effect of gut microflora on the digestibility of starch and proteins in young chicks","volume":"31","author":"Kussaibati","year":"1982","journal-title":"Ann. Zootech."},{"key":"10.3382\/ps\/pez466_bib24","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.yrtph.2017.07.014","article-title":"Safety evaluation of a novel muramidase for feed application","volume":"89","author":"Lichtenberg","year":"2017","journal-title":"Regul. Toxicol. Pharmacol."},{"key":"10.3382\/ps\/pez466_bib26","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1080\/03079450903447404","article-title":"Exogenous lysozyme influences Clostridium perfringens colonization and intestinal barrier function in broiler chickens","volume":"39","author":"Liu","year":"2010","journal-title":"Avian Pathol"},{"key":"10.3382\/ps\/pez466_bib27","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1111\/asj.12444","article-title":"Effects of dietary lysozyme levels on growth performance, intestinal morphology, non-specific immunity and mRNA expression in weanling piglets","volume":"87","author":"Long","year":"2016","journal-title":"Anim. Sci. J."},{"key":"10.3382\/ps\/pez466_bib28","doi-asserted-by":"crossref","first-page":"6816","DOI":"10.1128\/AEM.69.11.6816-6824.2003","article-title":"Diversity and succession of the intestinal bacterial community of the maturing broiler chicken","volume":"69","author":"Lu","year":"2003","journal-title":"Appl. Environ. Microbiol."},{"key":"10.3382\/ps\/pez466_bib29","first-page":"191","article-title":"Antimicrobial properties of lysozyme in relation to foodborne vegetative bacteria","volume":"29","author":"Masschalck","year":"2003","journal-title":"Microbiol"},{"key":"10.3382\/ps\/pez466_bib30","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1111\/j.1753-4887.2004.tb00031.x","article-title":"Effect of diet on mucin kinetics and composition: nutrition and health implications","volume":"62","author":"Montagne","year":"2004","journal-title":"Nutr. Rev."},{"key":"10.3382\/ps\/pez466_bib31","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1111\/j.1439-0388.2010.00866.x","article-title":"Striking the balance","volume":"127","author":"Neeteson","year":"2010","journal-title":"J. Anim. Breed. Genet."},{"key":"10.3382\/ps\/pez466_bib32","doi-asserted-by":"crossref","first-page":"366","DOI":"10.3382\/ps.0740366","article-title":"Digestion and absorption in the young chick","volume":"74","author":"Noy","year":"1995","journal-title":"Poult. Sci."},{"key":"10.3382\/ps\/pez466_bib33","doi-asserted-by":"crossref","first-page":"3129","DOI":"10.2527\/jas.2012-5782","article-title":"Lysozyme as an alternative to antibiotics improves growth performance and small intestinal morphology in nursery pigs","volume":"91","author":"Oliver","year":"2013","journal-title":"J. Anim. Sci."},{"key":"10.3382\/ps\/pez466_bib34","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1186\/s40104-015-0034-z","article-title":"Lysozyme as an alternative to growth promoting antibiotics in swine production","volume":"6","author":"Oliver","year":"2015","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"10.3382\/ps\/pez466_bib35","doi-asserted-by":"crossref","first-page":"G327","DOI":"10.1152\/ajpgi.00422.2010","article-title":"Importance and regulation of the colonic mucus barrier in a mouse model of colitis","volume":"300","author":"Petersson","year":"2011","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol."},{"key":"10.3382\/ps\/pez466_bib37","series-title":"R: A language and environment for statistical computing","author":"R Core Team","year":"2013"},{"key":"10.3382\/ps\/pez466_bib38","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.anifeedsci.2016.01.012","article-title":"Fats in poultry nutrition: Digestive physiology and factors influencing their utilization","volume":"213","author":"Ravindrana","year":"2016","journal-title":"Anim. Feed Sci. Technol."},{"key":"10.3382\/ps\/pez466_bib40","doi-asserted-by":"crossref","first-page":"e545","DOI":"10.7717\/peerj.545","article-title":"Subsampled open-reference clustering creates consistent, comprehensive OTU definitions and scales to billions of sequences","volume":"2","author":"Rideout","year":"2014","journal-title":"Peer. J."},{"key":"10.3382\/ps\/pez466_bib41","first-page":"347","article-title":"Lysozyme in livestock: A guide to selection for disease resistance","volume":"2","author":"Sahoo","year":"2012","journal-title":"J. Anim. Sci. Adv."},{"key":"10.3382\/ps\/pez466_bib42","doi-asserted-by":"crossref","first-page":"1415","DOI":"10.3382\/ps.2006-00462","article-title":"Change in the ileal bacterial population of turkeys fed different diets and after infection with Salmonella as determined with denaturing gradient gel electrophoresis of amplified 16S ribosomal DNA","volume":"87","author":"Santos","year":"2008","journal-title":"Poult. Sci."},{"key":"10.3382\/ps\/pez466_bib44","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1038\/ni.2029","article-title":"Regional and mucosal memory T cells","volume":"12","author":"Sheridan","year":"2011","journal-title":"Nat. Immunol."},{"key":"10.3382\/ps\/pez466_bib45","doi-asserted-by":"crossref","first-page":"387","DOI":"10.3389\/fcimb.2017.00387","article-title":"Interactions of intestinal bacteria with components of the intestinal mucus","volume":"5","author":"Sicard","year":"2017","journal-title":"Front. Cell Infect. Microbiol."},{"key":"10.3382\/ps\/pez466_bib46","doi-asserted-by":"crossref","first-page":"1202","DOI":"10.1021\/jf00084a019","article-title":"Rapid method for determination of total fatty acid content and composition of feedstuffs and feces","volume":"36","author":"Sukhija","year":"1988","journal-title":"J. Agric. Food Chem."},{"key":"10.3382\/ps\/pez466_bib47","doi-asserted-by":"crossref","first-page":"2052","DOI":"10.3382\/ps.2008-00091","article-title":"Factors affecting intestinal health in poultry","volume":"87","author":"Yegani","year":"2008","journal-title":"Poult. Sci."}],"container-title":["Poultry Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0032579119578683?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0032579119578683?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/academic.oup.com\/ps\/advance-article-pdf\/doi\/10.3382\/ps\/pez466\/30795528\/pez466.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/ps\/advance-article-pdf\/doi\/10.3382\/ps\/pez466\/30795528\/pez466.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,21]],"date-time":"2025-09-21T20:27:45Z","timestamp":1758486465000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0032579119578683"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1]]},"references-count":42,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,1]]}},"alternative-id":["S0032579119578683"],"URL":"https:\/\/doi.org\/10.3382\/ps\/pez466","relation":{},"ISSN":["0032-5791"],"issn-type":[{"value":"0032-5791","type":"print"}],"subject":[],"published":{"date-parts":[[2020,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Evaluation of dietary supplementation of a novel microbial muramidase on gastrointestinal functionality and growth performance in broiler chickens","name":"articletitle","label":"Article Title"},{"value":"Poultry Science","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.3382\/ps\/pez466","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2019 The Author(s). Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}