{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T10:46:38Z","timestamp":1789987598583,"version":"4.0.1"},"reference-count":35,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2019,10,9]],"date-time":"2019-10-09T00:00:00Z","timestamp":1570579200000},"content-version":"vor","delay-in-days":2107,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Poultry Science"],"published-print":{"date-parts":[[2014,1]]},"DOI":"10.3382\/ps.2013-03372","type":"journal-article","created":{"date-parts":[[2014,1,17]],"date-time":"2014-01-17T08:50:55Z","timestamp":1389948655000},"page":"39-45","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":5,"title":["Immune response of broiler chickens immunized orally with the recombinant proteins flagellin and the subunit B of cholera toxin associated with Lactobacillus spp."],"prefix":"10.1016","volume":"93","author":[{"given":"A.A.S.","family":"Baptista","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.C.","family":"Donato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.C.O.D.","family":"Garcia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.A.M.","family":"Gon\u00e7alves","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.P.","family":"Coppola","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.S.","family":"Okamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.L.","family":"Sequeira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.L. Andreatti","family":"Filho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.3382\/ps.2013-03372_bib1","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/0022-1759(90)90029-U","article-title":"Removal of endotoxin from protein solutions by phase separation using Triton X-114","volume":"132","author":"Aida","year":"1990","journal-title":"J. Immunol. Methods"},{"key":"10.3382\/ps.2013-03372_bib2","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1016\/S1046-5928(02)00026-8","article-title":"Synthesis of cholera toxin B subunit gene: Cloning and expression of a functional 6XHis-tagged protein in Escherichia coli","volume":"25","author":"Ar\u00eaas","year":"2002","journal-title":"Protein Expr. Purif."},{"key":"10.3382\/ps.2013-03372_bib3","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.pep.2009.04.011","article-title":"Lincomycin-induced over-expression of mature recombinant cholera toxin B subunit and the holotoxin in Escherichia coli","volume":"67","author":"Arimitsu","year":"2009","journal-title":"Protein Expr. Purif."},{"key":"10.3382\/ps.2013-03372_bib4","doi-asserted-by":"crossref","first-page":"1373","DOI":"10.1016\/j.vaccine.2009.11.003","article-title":"CD8+ T cell adjuvant effects of Salmonella FliCd flagellin in live vaccine vectors oras purified protein","volume":"28","author":"Braga","year":"2010","journal-title":"Vaccine"},{"key":"10.3382\/ps.2013-03372_bib5","doi-asserted-by":"crossref","first-page":"1337","DOI":"10.1128\/CVI.00143-10","article-title":"Effects of Lactobacilli on cytokine expression by chicken spleen and cecal tonsil cells","volume":"17","author":"Brisbin","year":"2010","journal-title":"Clin. Vaccine Immunol."},{"key":"10.3382\/ps.2013-03372_bib6","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1017\/S146625230800145X","article-title":"Interactions between commensal bacteria and the gut-associated immune system of the chicken","volume":"9","author":"Brisbin","year":"2008","journal-title":"Anim. Health Res. Rev."},{"key":"10.3382\/ps.2013-03372_bib7","doi-asserted-by":"crossref","first-page":"852","DOI":"10.1128\/CVI.00382-07","article-title":"Comparison of serum humoral responses induced by oral immunization with the hepatitis b virus core antigen and the cholera toxin b subunit","volume":"15","author":"Broos","year":"2008","journal-title":"Clin. Vaccine Immunol."},{"key":"10.3382\/ps.2013-03372_bib8","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.vetmic.2011.12.014","article-title":"Salmonella flagellin enhances mucosal immunity of avian influenza vaccine in chickens","volume":"157","author":"Chaung","year":"2012","journal-title":"Vet. Microbiol."},{"key":"10.3382\/ps.2013-03372_bib9","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1051\/vetres:2006014","article-title":"Adjuvants modulating mucosal immune responsesor directing systemic responses towards the mucosa","volume":"37","author":"Cox","year":"2006","journal-title":"Vet. Res."},{"key":"10.3382\/ps.2013-03372_bib10","doi-asserted-by":"crossref","first-page":"7009","DOI":"10.4049\/jimmunol.167.12.7009","article-title":"Salmonella flagellin-dependent proinflammatory responses are localized to the conserved amino and carboxyl regions of the protein","volume":"167","author":"Eaves-Pyles","year":"2001","journal-title":"J. Immunol."},{"key":"10.3382\/ps.2013-03372_bib11","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1093\/ps\/83.4.580","article-title":"Cell-mediated immunity in poultry","volume":"83","author":"Erf","year":"2004","journal-title":"Poult. Sci."},{"key":"10.3382\/ps.2013-03372_bib12","doi-asserted-by":"crossref","first-page":"4685","DOI":"10.1016\/j.vaccine.2005.03.050","article-title":"Vaccine and adjuvant activity of recombinant subunit B of E.coli enterotoxin produced in yeast","volume":"23","author":"Fingerut","year":"2005","journal-title":"Vaccine"},{"key":"10.3382\/ps.2013-03372_bib13","doi-asserted-by":"crossref","first-page":"2954","DOI":"10.1128\/JCM.02347-10","article-title":"Rapid genoserotyping tool for classification of Salmonella serovars","volume":"49","author":"Franklin","year":"2011","journal-title":"J. Clin. Microbiol."},{"key":"10.3382\/ps.2013-03372_bib14","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1128\/CVI.13.2.219-226.2006","article-title":"The probiotic bacterium Lactobacillus casei induces activation of the gut mucosal immune system through innate immunity","volume":"13","author":"Galdeano","year":"2006","journal-title":"Clin. Vaccine Immunol."},{"key":"10.3382\/ps.2013-03372_bib15","doi-asserted-by":"crossref","first-page":"1516","DOI":"10.1016\/S0264-410X(98)00364-8","article-title":"Adjuvant effect of cholera toxin on systemic and mucosal immune responses in chickens infected with E. tenella or given recombinant parasitic antigen per os","volume":"17","author":"Girard","year":"1999","journal-title":"Vaccine"},{"key":"10.3382\/ps.2013-03372_bib16","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1128\/CVI.00161-06","article-title":"Probiotics stimulate production of natural antibodies in chickens","volume":"13","author":"Haghighi","year":"2006","journal-title":"Clin. Vaccine Immunol."},{"key":"10.3382\/ps.2013-03372_bib17","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/j.imlet.2004.11.009","article-title":"Mucosal adjuvants and anti-infection and anti-immunopathology vaccinesbased on cholera toxin, cholera toxin B subunit and CpG DNA","volume":"97","author":"Holmgren","year":"2005","journal-title":"Immunol. Lett."},{"key":"10.3382\/ps.2013-03372_bib18","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1007\/s00418-009-0635-8","article-title":"Selection of buffer pH by the isoelectric point of the antigen for the effcient heat-induced epitope retrieval: Re-appraisal for nuclear protein pathobiology","volume":"132","author":"Kajiya","year":"2009","journal-title":"Histochem. Cell Biol."},{"key":"10.3382\/ps.2013-03372_bib19","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.cyto.2010.12.014","article-title":"Sublingual mucosa: A new vaccination route for systemic and mucosal immunity","volume":"54","author":"Kweon","year":"2011","journal-title":"Cytokine"},{"key":"10.3382\/ps.2013-03372_bib20","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1016\/j.coi.2011.03.009","article-title":"Defending the mucosa: Adjuvant and carrier formulations for mucosal immunity","volume":"23","author":"Lawson","year":"2011","journal-title":"Curr. Opin. Immunol."},{"key":"10.3382\/ps.2013-03372_bib21","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1016\/j.bbrc.2010.01.077","article-title":"Recombinant flagellins with partial deletions of the hypervariable domain lose antigenicity but not mucosal adjuvancy","volume":"392","author":"Liu","year":"2010","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.3382\/ps.2013-03372_bib22","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/S0145-305X(99)00081-6","article-title":"Immunity, vaccination and the avian intestinal tract","volume":"24","author":"Muir","year":"2000","journal-title":"Dev. Comp. Immunol."},{"key":"10.3382\/ps.2013-03372_bib23","doi-asserted-by":"crossref","first-page":"2036","DOI":"10.4049\/jimmunol.181.3.2036","article-title":"Deletion of flagellins hypervariable region abrogates antibody-mediated neutralization and systemic activation of TLR5-dependent immunity","volume":"181","author":"Nempont","year":"2008","journal-title":"J. Immunol."},{"key":"10.3382\/ps.2013-03372_bib24","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1590\/S1516-635X2009000300008","article-title":"Detection of CD4+ and CD8+ lymphocytes in the intestine of broiler chicks treated with Lactobacillus spp. and challenged with Salmonella enteric serovar Enteritidis","volume":"11","author":"Noujaim","year":"2009","journal-title":"Braz. J. Poult. Sci."},{"key":"10.3382\/ps.2013-03372_bib25","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1128\/CMR.14.2.430-445.2001","article-title":"Vaccination strategies for mucosal immune responses","volume":"14","author":"Ogra","year":"2001","journal-title":"Clin. Microbiol. Rev."},{"key":"10.3382\/ps.2013-03372_bib26","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1590\/S0104-66322011000400003","article-title":"Production of native flagellin from Salmonella typhimurium in a bioreactor and purification by tangential ultrafiltration","volume":"28","author":"Oliveira","year":"2011","journal-title":"Braz. J. Chem. Eng."},{"key":"10.3382\/ps.2013-03372_bib27","doi-asserted-by":"crossref","first-page":"362","DOI":"10.3382\/ps.2011-01803","article-title":"Evaluation of in vitro and in vivo adhesion and immunomodulatory effect of Lactobacillus species strains isolated from chickens","volume":"91","author":"Rocha","year":"2012","journal-title":"Poult. Sci."},{"key":"10.3382\/ps.2013-03372_bib28","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.imlet.2005.05.004","article-title":"Salmonella flagellin, a microbial target of the innate and adaptive immune system","volume":"101","author":"Salazar-Gonzalez","year":"2005","journal-title":"Immunol. Lett."},{"key":"10.3382\/ps.2013-03372_bib29","first-page":"711","article-title":"Cholera toxin b-subunit gene enhances mucosal immunoglobulin A, Th1-type, and CD8- cytotoxic responses when coadministered intradermally with a DNA vaccine","volume":"11","author":"Sanchez","year":"2004","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"10.3382\/ps.2013-03372_bib30","doi-asserted-by":"crossref","first-page":"1347","DOI":"10.1007\/s00018-008-7496-5","article-title":"Cholera toxin structure, gene regulation and pathophysiological and immunological aspects","volume":"65","author":"S\u00e1nchez","year":"2008","journal-title":"Cell. Mol. Life Sci."},{"key":"10.3382\/ps.2013-03372_bib31","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1111\/j.1574-695X.2001.tb01571.x","article-title":"Flagellin expressed by live Salmonella vaccine strains induces distinct antibody responses following delivery via systemic or mucosal immunization routes","volume":"30","author":"Sbrogio-Almeida","year":"2001","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"10.3382\/ps.2013-03372_bib32","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1051\/vetres:2006010","article-title":"The IgA system: A comparison of structure and function in different species","volume":"37","author":"Snoeck","year":"2006","journal-title":"Vet. Res."},{"key":"10.3382\/ps.2013-03372_bib33","doi-asserted-by":"crossref","first-page":"3797","DOI":"10.1016\/j.vaccine.2003.12.035","article-title":"Mucosal immunization with purified flagellin from Salmonella induces systemic and mucosal immune responses in C3H\/HeJ mice","volume":"22","author":"Strindelius","year":"2004","journal-title":"Vaccine"},{"key":"10.3382\/ps.2013-03372_bib34","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1365-3083.2009.02321.x","article-title":"Mucosally induced immunological tolerance, regulatory T cells and the adjuvant effect by cholera toxin B subunit","volume":"71","author":"Sun","year":"2010","journal-title":"Scand. J. Immunol."},{"key":"10.3382\/ps.2013-03372_bib35","doi-asserted-by":"crossref","first-page":"4734","DOI":"10.1016\/j.vaccine.2005.05.004","article-title":"Effectiveness of the B subunit of cholera toxin in potentiating immune responses to the recombinant hemagglutinin\/adhesin domain of the gingipain Kgp from Porphyromonas gingivalis","volume":"23","author":"Zhang","year":"2005","journal-title":"Vaccine"}],"container-title":["Poultry Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0032579119359802?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0032579119359802?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/academic.oup.com\/ps\/article-pdf\/93\/1\/39\/4405796\/39.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,21]],"date-time":"2025-09-21T21:02:07Z","timestamp":1758488527000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0032579119359802"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,1]]},"references-count":35,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2014,1]]}},"alternative-id":["S0032579119359802"],"URL":"https:\/\/doi.org\/10.3382\/ps.2013-03372","relation":{},"ISSN":["0032-5791"],"issn-type":[{"value":"0032-5791","type":"print"}],"subject":[],"published":{"date-parts":[[2014,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Immune response of broiler chickens immunized orally with the recombinant proteins flagellin and the subunit B of cholera toxin associated with Lactobacillus spp.","name":"articletitle","label":"Article Title"},{"value":"Poultry Science","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.3382\/ps.2013-03372","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2014 Poultry Science Association Inc. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}