{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T10:28:22Z","timestamp":1775816902362,"version":"3.50.1"},"reference-count":44,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,7,7]],"date-time":"2025-07-07T00:00:00Z","timestamp":1751846400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Bioinform."],"abstract":"<jats:p>Triterpenoid saponins are one of the main activities of roots and rhizomes of <jats:italic>Hylomecon japonica<\/jats:italic>, with various pharmacological activities such as antibacterial, anticancer, and anti-inflammatory. To elucidate the biosynthesis pathway of triterpenoid saponins in <jats:italic>H. japonica<\/jats:italic>, DNA nanoball sequencing technology was used to analyze the transcriptome of leaves, roots, and stems of <jats:italic>H. japonica<\/jats:italic>. Out of a total of 99,404 unigenes, 78,989 unigenes were annotated by seven major databases; 49 unigenes encoded 11 key enzymes in the biosynthesis pathway of triterpenoid saponins. Nine transcription factors were found to be involved in the metabolism of terpenoids and polyketides in <jats:italic>H<\/jats:italic>. <jats:italic>japonica<\/jats:italic> and a spatial structure model of squalene synthase in triterpenoid saponin biosynthesis was established. This study greatly enriched the transcriptome data of <jats:italic>H. japonica<\/jats:italic>, which is helpful for further analysis of the functions and regulatory mechanisms of key enzymes in the biosynthesis pathway of triterpenoid saponins.<\/jats:p>","DOI":"10.3389\/fbinf.2025.1625145","type":"journal-article","created":{"date-parts":[[2025,7,7]],"date-time":"2025-07-07T05:28:01Z","timestamp":1751866081000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Comparative transcriptome analysis of different tissues of Hylomecon japonica provides new insights into the biosynthesis pathway of triterpenoid saponins"],"prefix":"10.3389","volume":"5","author":[{"given":"Bing","family":"He","sequence":"first","affiliation":[]},{"given":"Teng","family":"Xu","sequence":"additional","affiliation":[]},{"given":"Shaowei","family":"Xu","sequence":"additional","affiliation":[]},{"given":"Huqiang","family":"Fang","sequence":"additional","affiliation":[]},{"given":"Qingshan","family":"Yang","sequence":"additional","affiliation":[]}],"member":"1965","published-online":{"date-parts":[[2025,7,7]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic local alignment search tool","volume":"215","author":"Altschul","year":"1990","journal-title":"J. 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