{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T10:05:54Z","timestamp":1771927554845,"version":"3.50.1"},"reference-count":24,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T00:00:00Z","timestamp":1769472000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Bioinform."],"abstract":"\n Background<\/jats:title>\n Lumbrokinase is a novel antithrombotic drug isolated and purified from earthworms and used in the treatment of stroke and cardiovascular disease. However, to date, there has been no systematic classification of lumbrokinase genes.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n In this study, a new member of the lumbrokinase gene family\n LUKA<\/jats:italic>\n , which has not yet been reported, was cloned from\n Eisenia andrei<\/jats:italic>\n by a homologous sequence method. The full-length cDNA sequence of\n LUKA<\/jats:italic>\n was obtained through second-generation sequencing. The gene and protein were analyzed using various bioinformatics software tools.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n The molecular formula of LUKA was C\n 1347<\/jats:sub>\n H\n 2068<\/jats:sub>\n N\n 360<\/jats:sub>\n O\n 419<\/jats:sub>\n S\n 18<\/jats:sub>\n , indicating it is a stable protein. The amino acid sequence of LUKA contained high proportion of valine (10.2%) and serine (9.2%) and possessed a peptide signal along with the corresponding cleavage site.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n Phylogenetic analysis showed that LUKA has multiple distinct amino acid sites compared with other lumbrokinases and represents a rare type of lumbrokinase. This study provides a new genetic material of rare lumbrokinase types.<\/jats:p>\n <\/jats:sec>","DOI":"10.3389\/fbinf.2026.1736746","type":"journal-article","created":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T06:42:26Z","timestamp":1769496146000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification and bioinformatics analysis of a novel member of the lumbrokinase gene family in earthworms"],"prefix":"10.3389","volume":"6","author":[{"given":"Xiong","family":"Rongchuan","sequence":"first","affiliation":[{"name":"School of Biological Science and Technology, Liupanshui Normal University","place":["Liupanshui, Guizhou, China"]}]},{"given":"Li","family":"Jianhong","sequence":"additional","affiliation":[{"name":"School of Biological Science and Technology, Liupanshui Normal University","place":["Liupanshui, Guizhou, China"]}]},{"given":"Liu","family":"Tingchang","sequence":"additional","affiliation":[{"name":"School of Biological Science and Technology, Liupanshui Normal University","place":["Liupanshui, Guizhou, China"]}]},{"given":"Su","family":"Chengyuan","sequence":"additional","affiliation":[{"name":"School of Biological Science and Technology, Liupanshui Normal University","place":["Liupanshui, Guizhou, China"]}]},{"given":"Chen","family":"Hong","sequence":"additional","affiliation":[{"name":"School of Biological Science and Technology, Liupanshui Normal University","place":["Liupanshui, Guizhou, China"]}]},{"given":"Chen","family":"Zhengpeng","sequence":"additional","affiliation":[{"name":"School of Biological Science and Technology, Liupanshui Normal University","place":["Liupanshui, Guizhou, China"]}]},{"given":"Tang","family":"Chaozhi","sequence":"additional","affiliation":[{"name":"School of Biological Science and Technology, Liupanshui Normal University","place":["Liupanshui, Guizhou, China"]}]},{"given":"Zhao","family":"Xinxia","sequence":"additional","affiliation":[{"name":"School of Biological Science and Technology, Liupanshui Normal University","place":["Liupanshui, Guizhou, China"]}]}],"member":"1965","published-online":{"date-parts":[[2026,1,27]]},"reference":[{"key":"B1","first-page":"1899","article-title":"Effects of earthworm ecotype and density on comprehensive quality of watered land soil","volume":"53","author":"Chen","year":"2022","journal-title":"J. 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