{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,10]],"date-time":"2025-11-10T06:15:04Z","timestamp":1762755304379,"version":"build-2065373602"},"reference-count":32,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,11,10]],"date-time":"2025-11-10T00:00:00Z","timestamp":1762732800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontierspartnerships.org"],"crossmark-restriction":true},"short-container-title":["Acta Biochim. Pol."],"abstract":"<jats:p>\n                    The aim of our work was to analyze new functionalized analogues of 5-substituted-2(1\n                    <jats:italic>H<\/jats:italic>\n                    )-pyridone containing of natural amino acids derivatives as a potential drugs in idiopathic pulmonary fibrosis (IPF). The creation of connections with natural amino acids was aimed at obtaining anti-fibrotic compounds with better water solubility, increased hydrophilicity, lower toxicity and better pharmacokinetic properties. For the docking studies the corresponding grid box parameters were used: PARP\u03b3, ALK5 andp38. During our initial research we have synthesized and performed biological\n                    <jats:italic>in vitro<\/jats:italic>\n                    studies for two analogues selected on the basis of molecular modeling:\n                    <jats:bold>6b<\/jats:bold>\n                    and\n                    <jats:bold>6f<\/jats:bold>\n                    . MTT test have been performed to select concentrations of PFD derivatives for subsequent analysis. We have analyzed HLA-DR and CXCR4 expression on fibroblasts and 24\u00a0h migration of TGF-\u03b21-stimulated fibroblasts. We have also explored proliferation and production of TGF-\u03b21 as well as IL-17 by CD3\/CD28 beads-stimulated PBMCs. Preliminary studies show that the designed compounds exhibit promising potential as anti-fibrotic therapeutics. Therefore, their activity is worth further exploring.\n                  <\/jats:p>","DOI":"10.3389\/abp.2025.15218","type":"journal-article","created":{"date-parts":[[2025,11,10]],"date-time":"2025-11-10T06:10:16Z","timestamp":1762755016000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["New analogs of 5-substituted-2(1H)-pyridone containing of natural amino acids as potential drugs in idiopathic pulmonary fibrosis. Investigation in silico and preliminary in vitro"],"prefix":"10.3389","volume":"72","author":[{"given":"Krystyna","family":"Dzierzbicka","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Skrzypkowska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monika","family":"Gensicka-Kowalewska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mateusz","family":"Da\u015bko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bartosz","family":"S\u0142omi\u0144ski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2025,11,10]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"577","DOI":"10.1007\/s00441-017-2748-z","article-title":"Neutrophils in the lung: \u201cthe first responders\u201d","volume":"371","author":"Aulakh","year":"2018","journal-title":"Cell Tissue Res."},{"key":"B2","doi-asserted-by":"publisher","first-page":"100002","DOI":"10.1016\/j.yrmex.2019.100002","article-title":"T cell co-stimulatory molecules ICOS and CD28 stratify idiopathic pulmonary fibrosis survival","volume":"1","author":"Bonham","year":"2019","journal-title":"Respir. 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