{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T06:04:41Z","timestamp":1784268281280,"version":"3.55.0"},"reference-count":44,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T00:00:00Z","timestamp":1784246400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontierspartnerships.org"],"crossmark-restriction":true},"short-container-title":["Adv. Drug Alcohol Res."],"abstract":"<jats:p>\n                    Binge alcohol drinking is significantly associated with an increased risk of cerebrovascular dysfunction. Within cerebral artery smooth muscle, alcohol inhibits calcium- and voltage-gated potassium (BK) channels of large-conductance, leading to cerebrovascular constriction. Administration of high-cholesterol diet or docosahexaenoic acid (DHA) supplementation independently protects against alcohol-induced constriction of cerebral arteries. However, whether these interventions act additively or through a shared mechanism(s) remains unclear. Here, we examined the combined effects of cholesterol and DHA dietary supplementation\n                    <jats:italic>in vivo<\/jats:italic>\n                    and their combined administration\n                    <jats:italic>ex vivo<\/jats:italic>\n                    on alcohol-induced changes in rat cerebral artery diameter. DHA dietary supplementation\n                    <jats:italic>in vivo<\/jats:italic>\n                    and application to rat cerebral arteries\n                    <jats:italic>ex vivo<\/jats:italic>\n                    did not further modify protective action of high-cholesterol diet and cholesterol-enrichment, respectively. Lack of additivity may point to a shared mechanism(s) that drives cholesterol- and DHA-driven protection against alcohol constriction of cerebral arteries. Considering a prior report of BK channel \u03b1-subunit (slo1) residue tyrosine 450 mediating cholesterol protection against alcohol cerebrovascular action, we probed slo1Y450 role in DHA protection against alcohol effect on cerebral artery diameter. DHA protection against alcohol-induced constriction vanished in cerebral arteries harvested from male slo1Y450F knock-in mice. Yet DHA protection against alcohol was still present in arteries harvested from female slo1Y450F knock-in mice. Combined with previous observations, our current findings identify slo1Y450 as a potentially shared target involved in a sexually dimorphic pathway between alcohol effect on cerebral artery diameter and its sensitivity to cholesterol- and DHA-driven interventions.\n                  <\/jats:p>","DOI":"10.3389\/adar.2026.16302","type":"journal-article","created":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T05:10:09Z","timestamp":1784265009000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Cholesterol and docosahexaenoic acid likely protect against alcohol-induced constriction of cerebral arteries via a common pathway"],"prefix":"10.3389","volume":"6","author":[{"given":"Shiwani","family":"Thapa","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Addiction Science, and Toxicology College of Medicine, The University of Tennessee Health Science Center","place":["Memphis, TN, United 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