{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T20:08:43Z","timestamp":1789157323579,"version":"build-2803163510"},"reference-count":35,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,6,23]],"date-time":"2022-06-23T00:00:00Z","timestamp":1655942400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontierspartnerships.org"],"crossmark-restriction":true},"short-container-title":["Br J Biomed Sci"],"abstract":"<jats:p><jats:bold>Introduction:<\/jats:bold> Long non-coding RNAs (lncRNAs) exhibit crucial roles in human tumors. However, the role of lncRNA CARD8-AS1 in lung adenocarcinoma remains elusive. This study investigated the role of CARD8-AS1 in lung adenocarcinoma.<\/jats:p><jats:p><jats:bold>Materials and Methods:<\/jats:bold> The expression of CARD8-AS1 was detected by RT-qPCR analysis and confirmed using an online database. The clinical value of CARD8-AS1 was evaluated using the Kaplan-Meier curve and multivariate Cox regression analyses. The effects of CARD8-AS1 on cancer cell proliferation, migration, and invasion potential were assessed through several cellular experiments. Western blot assay was used to measure Bcl-2 and Bax protein levels. The interaction among CARD8-AS1, miR-650, and Bax, was assessed using a dual-luciferase reporter assay.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> The expression of CARD8-AS1 was decreased in lung adenocarcinoma tissues and cell lines (<jats:italic>p<\/jats:italic> &amp;lt; 0.001). Low expression of CARD8-AS1 was related to tumor size (<jats:italic>p<\/jats:italic> = 0.042), TNM stage (<jats:italic>p<\/jats:italic> = 0.021), lymph node metastasis (<jats:italic>p<\/jats:italic> = 0.025), and poor overall survival (<jats:italic>p<\/jats:italic> &amp;lt; 0.05). Elevated expression of CARD8-AS1 could suppress cellular viability, migration potential, and invasion ability (<jats:italic>p<\/jats:italic> &amp;lt; 0.05). The Bcl-2 protein levels were decreased while Bax levels were increased by overexpression of CARD8-AS1 (<jats:italic>p<\/jats:italic> &amp;lt; 0.001). miR-650 may thus be a direct target of CARD8-AS1 and Bax may be a direct target of miR-650.<\/jats:p><jats:p><jats:bold>Discussion:<\/jats:bold> CARD8-AS1 expression was downregulated in lung adenocarcinoma and associated with several clinical parameters. CARD8-AS1 exerted tumor-suppressive effects by targeting the miR-650 and then regulating Bax expression. CARD8-AS1\/miR-650 may serve as novel prognostic biomarkers and potential therapeutic targets for the treatment of lung adenocarcinoma.<\/jats:p>","DOI":"10.3389\/bjbs.2022.10498","type":"journal-article","created":{"date-parts":[[2022,6,23]],"date-time":"2022-06-23T09:46:37Z","timestamp":1655977597000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["Identification of LncRNA CARD8-AS1 as a Potential Prognostic Biomarker Associated With Progression of Lung 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