{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T06:04:15Z","timestamp":1780639455578,"version":"3.54.1"},"reference-count":26,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T00:00:00Z","timestamp":1780617600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Allergy"],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Aspergillus fumigatus<\/jats:italic>\n                      is a major cause of allergic bronchopulmonary aspergillosis (ABPA), yet diagnosis remains challenging due to overlap with other atopic conditions. Asp f 10, a secreted aspartic protease, is listed as an allergen but remains poorly characterized in terms of biochemical properties and diagnostic relevance.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We profiled the\n                      <jats:italic>A. fumigatus<\/jats:italic>\n                      secretome under host-mimicking conditions to identify IgG-reactive proteins, followed by cloning and recombinant expression of full-length and mature Asp f 10. Biochemical stability, enzymatic activity, and serological reactivity (IgE and IgG) were evaluated using sera from patients with ABPA, steroid-unresponsive severe asthma with\n                      <jats:italic>Aspergillus<\/jats:italic>\n                      sensitization (SAAS), and other allergic and non-allergic controls. Diagnostic performance was assessed by ROC and linear discriminant analyses.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Asp f 10 was identified as an extracellular IgG-reactive protease. Recombinant mature Asp f 10 exhibited folded conformation, structural stability, and gelatinolytic activity, whereas the full-length protein lacked defined structure. Both isoforms bound patient IgE and IgG; however, only Asp f 10\u2013specific IgG responses were significantly elevated in ABPA and SAAS. ROC analysis demonstrated excellent diagnostic capacity for IgG (AUC\u2009=\u20090.90), far surpassing IgE (AUC\u2009=\u20090.54). Despite IgE recognition, mature Asp f 10 failed to induce histamine release, suggesting limited effector activity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Asp f 10 is a biologically active protease, and Asp f 10\u2013specific IgG responses provide a robust biomarker for ABPA, outperforming IgE in diagnostic accuracy. Incorporating Asp f 10 into serological panels may improve differential diagnosis of\n                      <jats:italic>Aspergillus<\/jats:italic>\n                      -associated respiratory diseases.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/falgy.2026.1733299","type":"journal-article","created":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T05:36:48Z","timestamp":1780637808000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Serological and biochemical characterization of Aspergillus fumigatus Asp f 10 as a potential diagnostic marker in ABPA and related respiratory diseases"],"prefix":"10.3389","volume":"7","author":[{"given":"Shramana","family":"Ghosh","sequence":"first","affiliation":[{"name":"Department of Botany, Visva-Bharati","place":["Santiniketan, West Bengal, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angira","family":"Dasgupta","sequence":"additional","affiliation":[{"name":"Department of Chest Medicines, B. 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