{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T13:48:14Z","timestamp":1785937694866,"version":"3.56.0"},"reference-count":39,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:00:00Z","timestamp":1785888000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Allergy"],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Diagnosis and allergen-specific immunotherapy for Mediterranean allergy rely on extracts standardized to group-1 allergens, such as Cup a 1 from\n                      <jats:italic>Cupressus arizonica<\/jats:italic>\n                      , potentially overlooking interspecies variability. The native species\n                      <jats:italic>Juniperus oxycedrus<\/jats:italic>\n                      , widespread in Spain, may contribute to sensitization, but its clinical relevance and allergen repertoire remain poorly characterized.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      To characterize and compare the allergen and IgE-binding protein composition of\n                      <jats:italic>J. oxycedrus<\/jats:italic>\n                      and\n                      <jats:italic>C. arizonica<\/jats:italic>\n                      pollen extracts and assess their sensitizing relevance in Spain.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Pollen extracts were analyzed using SDS-PAGE, two-dimensional electrophoresis (2-DE), Western blotting (WB), and mass spectrometry (MS). 241 patients with allergic rhinitis and\/or conjunctivitis from seven Spanish regions underwent skin prick testing and specific IgE for extracts and Jun o 1\/Cup a 1, by ELISA. Cross-reactivity was assessed by competitive ELISA and WB inhibition.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Proteomics and 2-DE showed a more complex protein profile in\n                      <jats:italic>J. oxycedrus<\/jats:italic>\n                      than in\n                      <jats:italic>C. arizonica<\/jats:italic>\n                      pollen extract (423 vs. 251 proteins, 146 shared). Among patients, 35% were sensitized to Cupressaceae, with more frequent monosensitization to\n                      <jats:italic>J. oxycedrus<\/jats:italic>\n                      . 2-DE\/WB showed \u226560 IgE-binding spots in\n                      <jats:italic>J. oxycedrus<\/jats:italic>\n                      vs. 5 in\n                      <jats:italic>C. arizonica<\/jats:italic>\n                      , including group-1 allergen isoforms and IgE-reactive components (calreticulin, superoxide dismutase, aconitate hydratase, aldose 1-epimerase, and enolase) with lower abundance in\n                      <jats:italic>C. arizonica<\/jats:italic>\n                      . Inhibition assays indicated high but asymmetric cross-reactivity, consistent with the differences in IgE-binding protein abundance.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      <jats:italic>J. oxycedrus<\/jats:italic>\n                      and\n                      <jats:italic>C. arizonica<\/jats:italic>\n                      pollen extracts display broadly similar IgE-reactive repertoires but differ in the relative abundance. Such differences may affect regional sensitization and limit the effectiveness of group 1\u2013based diagnosis and therapy.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/falgy.2026.1885294","type":"journal-article","created":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T13:19:34Z","timestamp":1785935974000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Proteomic profiling of Juniperus oxycedrus and Cupressus arizonica pollen reveals quantitative interspecies differences in the IgE-binding protein repertoire associated with Mediterranean Cupressaceae allergy"],"prefix":"10.3389","volume":"7","author":[{"given":"Sandra","family":"Sivill","sequence":"first","affiliation":[{"name":"Inmunotek, S.L., R&D Department, Alcal\u00e1 de Henares","place":["Madrid, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose Carlos","family":"Hern\u00e1ndez-Walias","sequence":"additional","affiliation":[{"name":"Inmunotek, S.L., R&D Department, Alcal\u00e1 de Henares","place":["Madrid, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Manuel","family":"Igea","sequence":"additional","affiliation":[{"name":"Allergy Unit, Cl\u00ednica Alergoasma","place":["Salamanca, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose Alejandro","family":"Lemus Calderon","sequence":"additional","affiliation":[{"name":"Allergy Unit, Hospital General de Villarobledo","place":["Albacete, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Maria","family":"Navarro-Pulido","sequence":"additional","affiliation":[{"name":"Allergy Unit, Hospital Universitario Virgen Macarena","place":["Sevilla, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Rondon","sequence":"additional","affiliation":[{"name":"Allergy Unit, Hospital Regional Universitario de Malaga","place":["Malaga, Spain"]},{"name":"Allergy Group. Instituto de Investigaci\u00f3n Biom\u00e9dica de M\u00e1laga y Plataforma en Nanomedicina - IBIMA Plataforma BIONAND. RICORS Inflammatory Diseases","place":["Malaga, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatriz","family":"Fernandez Parra","sequence":"additional","affiliation":[{"name":"Allergy Unit, Hospital Universitario El Bierzo","place":["Ponferrada\/Le\u00f3n, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magdalena","family":"Lluch-Bernal","sequence":"additional","affiliation":[{"name":"Allergy Unit, Hospital Universitario La Paz","place":["Madrid, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Teresa","family":"Dordal Culla","sequence":"additional","affiliation":[{"name":"Allergy Unit, Hospital Universitari de Bellvitge, L'Hospitalet de Llobregat","place":["Barcelona, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"Pineda","sequence":"additional","affiliation":[{"name":"Inmunotek, S.L., R&D Department, Alcal\u00e1 de Henares","place":["Madrid, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose Luis","family":"Subiza","sequence":"additional","affiliation":[{"name":"Inmunotek, S.L., R&D Department, Alcal\u00e1 de Henares","place":["Madrid, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salvador","family":"Iborra","sequence":"additional","affiliation":[{"name":"Inmunotek, S.L., R&D Department, Alcal\u00e1 de Henares","place":["Madrid, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose 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