{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T12:42:49Z","timestamp":1791895369991,"version":"4.3.4"},"reference-count":58,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2020,6,16]],"date-time":"2020-06-16T00:00:00Z","timestamp":1592265600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010665","name":"H2020 Marie Sk\u00c5,odowska-Curie Actions","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010665","name":"H2020 Marie Sk\u00c5,odowska-Curie Actions","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Bioeng. Biotechnol."],"DOI":"10.3389\/fbioe.2020.00629","type":"journal-article","created":{"date-parts":[[2020,6,16]],"date-time":"2020-06-16T04:33:33Z","timestamp":1592282013000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":16,"title":["Graphene Oxide Improves in vitro Fertilization in Mice With No Impact on Embryo Development and Preserves the Membrane Microdomains Architecture"],"prefix":"10.3389","volume":"8","author":[{"given":"Nicola","family":"Bernab\u00f2","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luca","family":"Valbonetti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcello","family":"Raspa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonella","family":"Fontana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Palestini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Botto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renata","family":"Paoletti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Fray","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan","family":"Allen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana","family":"Machado-Simoes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Ramal-Sanchez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serena","family":"Pilato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ferdinando","family":"Scavizzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Barboni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2020,6,16]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1038\/srep30270","article-title":"Toxicology study of single-walled carbon nanotubes and reduced graphene Oxide in human sperm.","volume":"6","author":"Asghar","year":"2016","journal-title":"Sci. Rep."},{"key":"B2","doi-asserted-by":"publisher","first-page":"660","DOI":"10.1093\/humupd\/dmx021","article-title":"The diagnosis of male infertility: an analysis of the evidence to support the development of global WHO guidance\u2014challenges and future research opportunities.","volume":"23","author":"Barratt","year":"2017","journal-title":"Hum. Reprod. Update"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.4155\/fso.15.63","article-title":"Animal models are essential to biological research: issues and perspectives.","volume":"1","author":"Barr\u00e9-Sinoussi","year":"2015","journal-title":"Futur. Sci. OA"},{"key":"B4","author":"Behringer","year":"2014","journal-title":"Manipulating the Mouse Embryo A Laboratory Manual"},{"key":"B5","doi-asserted-by":"publisher","first-page":"428","DOI":"10.1016\/j.carbon.2017.12.042","article-title":"Graphene Oxide affects in vitro fertilization outcome by interacting with sperm membrane in an animal model.","volume":"129","author":"Bernab\u00f2","year":"2018","journal-title":"Carbon N. Y."},{"key":"B6","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41598-019-44702-44705","article-title":"Graphene Oxide increases mammalian spermatozoa fertilizing ability by extracting cholesterol from their membranes and promoting capacitation.","volume":"9","author":"Bernab\u00f2","year":"2019","journal-title":"Sci. Rep."},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.112.101253","article-title":"Involvement of bicarbonate-induced radical signaling in oxysterol formation and sterol depletion of capacitating mammalian sperm during in vitro fertilization.","volume":"88","author":"Boerke","year":"2013","journal-title":"Biol. Reprod."},{"key":"B8","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1007\/s00232-010-9316-9318","article-title":"Bicarbonate induces membrane reorganization and CBR1 and TRPV1 endocannabinoid receptor migration in lipid microdomains in capacitating boar spermatozoa.","volume":"238","author":"Botto","year":"2010","journal-title":"J. Membr. Biol."},{"key":"B9","doi-asserted-by":"publisher","first-page":"7154","DOI":"10.1021\/acsnano.6b03438","article-title":"Graphene Oxide nanosheets disrupt lipid composition, ca 2+ homeostasis, and synaptic transmission in primary cortical neurons.","volume":"10","author":"Bramini","year":"2016","journal-title":"ACS Nano"},{"key":"B10","doi-asserted-by":"publisher","first-page":"668","DOI":"10.1034\/j.1600-0854.2001.20909.x","article-title":"Microdomains, lipid rafts and caveolae (San Feliu de Guixols, Spain, 19-24 May 2001).","volume":"2","author":"Brown","year":"2001","journal-title":"Traffic"},{"key":"B11","doi-asserted-by":"publisher","first-page":"1716","DOI":"10.1016\/j.theriogenology.2005.09.016","article-title":"Performance of ten inbred mouse strains following assisted reproductive technologies (ARTs).","volume":"65","author":"Byers","year":"2006","journal-title":"Theriogenology"},{"key":"B12","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/s0014-5793(04)00050-x","article-title":"Detergents as tools for the purification and classification of lipid rafts.","volume":"559","author":"Chamberlain","year":"2004","journal-title":"FEBS Lett."},{"key":"B13","doi-asserted-by":"publisher","first-page":"1328","DOI":"10.1095\/biolreprod59.6.1328","article-title":"Cyclodextrin removes cholesterol from mouse sperm and induces capacitation in a protein-free medium1.","volume":"59","author":"Choi","year":"1998","journal-title":"Biol. Reprod."},{"key":"B14","doi-asserted-by":"publisher","first-page":"649","DOI":"10.1530\/REP-12-0279","article-title":"Roles of the oviduct in mammalian fertilization.","volume":"144","author":"Coy","year":"2012","journal-title":"Reproduction"},{"key":"B15","doi-asserted-by":"publisher","first-page":"1586","DOI":"10.1093\/humrep\/dey242","article-title":"The European IVF-monitoring Consortium (EIM) \u2021 for the European society of human reproduction and embryology (ESHRE).","volume":"33","author":"De Geyter","year":"2018","journal-title":"Hum. Reprod."},{"key":"B16","doi-asserted-by":"publisher","first-page":"1586","DOI":"10.1093\/HROPEN\/HOZ038","article-title":"ART in Europe, 2015: results generated from European registries by ESHRE.","volume":"33","author":"De Geyter","year":"2020","journal-title":"Hum. Reprod. Open"},{"key":"B17","doi-asserted-by":"publisher","first-page":"348","DOI":"10.1002\/mrd.22970","article-title":"Long term effects of ART: what do animals tell us?","volume":"85","author":"Duranthon","year":"2018","journal-title":"Mol. Reprod. Dev."},{"key":"B18","doi-asserted-by":"publisher","first-page":"1091","DOI":"10.1038\/nm1001-1091","article-title":"The bumpy road to human in vitro fertilization.","volume":"7","author":"Edwards","year":"2001","journal-title":"Nature Med"},{"key":"B19","doi-asserted-by":"publisher","first-page":"733","DOI":"10.1002\/pc.22198","article-title":"Mechanical, thermal, and rheological properties of graphene-based polypropylene nanocomposites prepared by melt mixing.","volume":"33","author":"El Achaby","year":"2012","journal-title":"Polym. Compos"},{"key":"B20","doi-asserted-by":"publisher","first-page":"615","DOI":"10.1021\/acsnano.5b05647","article-title":"Graphene-based interfaces do not alter target nerve cells.","volume":"10","author":"Fabbro","year":"2016","journal-title":"ACS Nano"},{"key":"B21","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1002\/9780470942390.mo140059","article-title":"In vitro fertilization in mice using the MBCD-GSH protocol.","volume":"4","author":"Guan","year":"2014","journal-title":"Curr. Protoc. Mouse Biol."},{"key":"B22","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1002\/dvg.20598","article-title":"Genetic vasectomy - overexpression of Prm1-EGFP fusion protein in elongating spermatids causes dominant male sterility in mice.","volume":"48","author":"Haueter","year":"2010","journal-title":"Genesis"},{"key":"B23","doi-asserted-by":"publisher","first-page":"232","DOI":"10.1002\/mrd.1080410214","article-title":"Preimplantation development of mouse embryos in KSOM: augmentation by amino acids and analysis of gene expression.","volume":"41","author":"Ho","year":"1995","journal-title":"Mol. Reprod. Dev."},{"key":"B24","doi-asserted-by":"publisher","first-page":"694","DOI":"10.1093\/oxfordjournals.aje.a009511","article-title":"Comparison of population-averaged and subject-specific approaches for analyzing repeated binary outcomes.","volume":"147","author":"Hu","year":"1998","journal-title":"Am. J. Epidemiol."},{"key":"B25","doi-asserted-by":"publisher","first-page":"1339","DOI":"10.1021\/ja01539a017","article-title":"Preparation of graphitic Oxide.","volume":"80","author":"Hummers","year":"1958","journal-title":"J. Am. Chem. Soc."},{"key":"B26","doi-asserted-by":"publisher","first-page":"1135","DOI":"10.1095\/biolreprod21.5.1135","article-title":"Regulation of guinea pig sperm adenylate cyclase by calcium1.","volume":"21","author":"Hyne","year":"1979","journal-title":"Biol. Reprod."},{"key":"B27","doi-asserted-by":"publisher","first-page":"454","DOI":"10.1262\/jrd.2014-2045","article-title":"Prolonged exposure to hyaluronidase decreases the fertilization and development rates of fresh and cryopreserved mouse oocytes.","volume":"60","author":"Ishizuka","year":"2014","journal-title":"J. Reprod. Dev."},{"key":"B28","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1038\/nrmicro2314","article-title":"Regulation of the replication cycle: conserved and diverse regulatory systems for DnaA and oriC.","volume":"8","author":"Katayama","year":"2010","journal-title":"Nat. Rev. Microbiol."},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1000412","article-title":"Improving bioscience research reporting: the ARRIVE guidelines for reporting animal research.","volume":"8","author":"Kilkenny","year":"2010","journal-title":"PLoS Biol."},{"key":"B30","doi-asserted-by":"publisher","first-page":"5824","DOI":"10.3168\/jds.2016-12484","article-title":"Sperm viability, reactive oxygen species, and DNA fragmentation index combined can discriminate between above- and below-average fertility bulls.","volume":"100","author":"Kumaresan","year":"2017","journal-title":"J. Dairy Sci."},{"key":"B31","doi-asserted-by":"publisher","first-page":"561","DOI":"10.4103\/1008-682X.153309","article-title":"New insights into the regulation of cholesterol efflux from the sperm membrane.","volume":"17","author":"Leahy","year":"2015","journal-title":"Asian J. Androl."},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.4172\/2375-4508.1000175","article-title":"Cryorecovery of mouse sperm by different IVF methods using MBCD and GSH.","volume":"2016","author":"Li","year":"2016","journal-title":"J. Fertil. Vitr. IVF Worldwide Reprod. Med. Genet. Stem Cell Biol."},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1001356","article-title":"National, regional, and global trends in infertility prevalence since 1990: a systematic analysis of 277 health surveys.","volume":"9","author":"Mascarenhas","year":"2012","journal-title":"PLoS Med."},{"key":"B34","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1258\/jrsm.2007.070164","article-title":"Medical progress depends on animal models - Doesn\u2019t it?","volume":"101","author":"Matthews","year":"2008","journal-title":"J. R. Soc. Med."},{"key":"B35","doi-asserted-by":"publisher","first-page":"373","DOI":"10.1016\/0093-691X(96)81099-81095","article-title":"Identification of capacitation in boar spermatozoa by chlortetracycline staining.","volume":"45","author":"Mattioli","year":"1996","journal-title":"Theriogenology"},{"key":"B36","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1111\/j.1471-4159.2007.04720.x","article-title":"Lipid raft heterogeneity: an enigma.","author":"Mishra","year":"2007","journal-title":"J. Neurochem."},{"key":"B37","doi-asserted-by":"publisher","first-page":"122","DOI":"10.1002\/jcp.21575","article-title":"Composition and significance of detergent resistant membranes in mouse spermatozoa.","volume":"218","author":"Nixon","year":"2009","journal-title":"J. Cell. Physiol."},{"key":"B38","doi-asserted-by":"publisher","first-page":"192","DOI":"10.1038\/nature11458","article-title":"A roadmap for graphene.","volume":"490","author":"Novoselov","year":"2012","journal-title":"Nature"},{"key":"B39","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1201\/b12876-5","article-title":"Conserving, distributing and managing genetically modified mouse lines by sperm cryopreservation","author":"Ostermeier","year":"2011","journal-title":"Genetic Engineering: Recent Developments in Applications"},{"key":"B40","doi-asserted-by":"publisher","first-page":"170","DOI":"10.1093\/emph\/eow014","article-title":"Mouse models of human disease: an evolutionary perspective.","volume":"2016","author":"Perlman","year":"2016","journal-title":"Evol. Med. Public Heal"},{"key":"B41","doi-asserted-by":"publisher","first-page":"1597","DOI":"10.1194\/jlr.E600002-JLR200","article-title":"Rafts defined: a report on the keystone symposium on lipid rafts and cell function.","volume":"47","author":"Pike","year":"2006","journal-title":"J. Lipid Res."},{"key":"B42","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1111\/j.1471-4159.1980.tb06263.x","article-title":"Occurrence of sialyltransferase activity in the synaptosomal membranes prepared from calf brain cortex.","volume":"35","author":"Preti","year":"1980","journal-title":"J. Neurochem."},{"key":"B43","doi-asserted-by":"publisher","first-page":"518","DOI":"10.1016\/j.carbon.2019.05.055","article-title":"Graphene Oxide: a glimmer of hope for assisted reproductive technology.","volume":"150","author":"Ramal-Sanchez","year":"2019","journal-title":"Carbon N. Y."},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-08387-8388","article-title":"Pluripotency and X chromosome dynamics revealed in pig pre-gastrulating embryos by single cell analysis.","volume":"10","author":"Ramos-Ibeas","year":"2019","journal-title":"Nat. Commun."},{"key":"B45","doi-asserted-by":"publisher","first-page":"4459","DOI":"10.1021\/acsnano.6b00130","article-title":"Graphene Oxide nanosheets reshape synaptic function in cultured brain networks.","volume":"10","author":"Rauti","year":"2016","journal-title":"ACS Nano"},{"key":"B46","doi-asserted-by":"publisher","first-page":"902","DOI":"10.1136\/jmg.2006.042135","article-title":"The epigenetic imprinting defect of patients with Beckwitn-Wiedemann syndrome born after assisted reproductive technology is not restricted to the 11p15 region.","volume":"43","author":"Rossignol","year":"2006","journal-title":"J. Med. Genet."},{"key":"B47","doi-asserted-by":"publisher","first-page":"1909","DOI":"10.1007\/s00018-018-2765-2764","article-title":"Lipids in the cell: organisation regulates function.","volume":"75","author":"Santos","year":"2018","journal-title":"Cell. Mol. Life Sci."},{"key":"B48","doi-asserted-by":"publisher","first-page":"253","DOI":"10.1095\/biolreprod.103.026435","article-title":"Cholesterol efflux alters lipid raft stability and distribution during capacitation of boar spermatozoa1.","volume":"71","author":"Shadan","year":"2004","journal-title":"Biol. Reprod."},{"key":"B49","doi-asserted-by":"publisher","first-page":"3187","DOI":"10.1091\/mbc.10.10.3187","article-title":"Exogenous administration of gangliosides displaces GPI-anchored proteins from lipid microdomains in living cells.","volume":"10","author":"Simons","year":"1999","journal-title":"Mol. Biol. Cell"},{"key":"B50","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1177\/0023677217724823","article-title":"PREPARE: guidelines for planning animal research and testing.","volume":"52","author":"Smith","year":"2018","journal-title":"Lab. Anim."},{"key":"B51","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1038\/jhg.2013.51","article-title":"Epigenetic and genetic alterations of the imprinting disorder beckwith-wiedemann syndrome and related disorders.","volume":"58","author":"Soejima","year":"2013","journal-title":"J. Hum. Genet."},{"key":"B52","doi-asserted-by":"publisher","first-page":"2520","DOI":"10.1093\/humrep\/15.12.2520","article-title":"Selection of patients suitable for one-embryo transfer may reduce the rate of multiple births by half without impairment of overall birth rates.","volume":"15","author":"Strandell","year":"2000","journal-title":"Hum. Reprod."},{"key":"B53","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1007\/s00441-015-2244-2242","article-title":"Mammalian sperm interactions with the female reproductive tract.","volume":"363","author":"Suarez","year":"2016","journal-title":"Cell Tissue Res."},{"key":"B54","doi-asserted-by":"publisher","first-page":"546","DOI":"10.1095\/biolreprod.107.065359","article-title":"Methyl-beta-cyclodextrin improves fertilizing ability of C57BL\/6 mouse sperm after freezing and thawing by facilitating cholesterol efflux from the cells1.","volume":"78","author":"Takeo","year":"2008","journal-title":"Biol. Reprod."},{"key":"B55","doi-asserted-by":"publisher","first-page":"132","DOI":"10.1258\/la.2009.009074","article-title":"Combination medium of cryoprotective agents containing L-glutamine and methyl-\u03b2-cyclodextrin in a preincubation medium yields a high fertilization rate for cryopreserved C57BL\/6J mouse sperm.","volume":"44","author":"Takeo","year":"2010","journal-title":"Lab. Anim."},{"key":"B56","doi-asserted-by":"publisher","first-page":"160","DOI":"10.1016\/0005-2760(73)90055-90056","article-title":"A new procedure for the extraction, purification and fractionation of brain gangliosides.","volume":"296","author":"Tettamanti","year":"1973","journal-title":"Biochim. Biophys. Acta Lipids Lipid Metab."},{"key":"B57","doi-asserted-by":"publisher","first-page":"3906","DOI":"10.1002\/adma.201001068","article-title":"Graphene and graphene Oxide: synthesis. properties, and applications.","volume":"22","author":"Zhu","year":"2010","journal-title":"Adv. Mater."},{"key":"B58","author":"Zurutuza Elorza","year":"2016","journal-title":"Method for Obtaining Graphene Oxide."}],"container-title":["Frontiers in Bioengineering and Biotechnology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fbioe.2020.00629\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,6,16]],"date-time":"2020-06-16T04:33:36Z","timestamp":1592282016000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fbioe.2020.00629\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6,16]]},"references-count":58,"alternative-id":["10.3389\/fbioe.2020.00629"],"URL":"https:\/\/doi.org\/10.3389\/fbioe.2020.00629","relation":{},"ISSN":["2296-4185"],"issn-type":[{"value":"2296-4185","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,6,16]]},"article-number":"629"}}