{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T22:19:00Z","timestamp":1793053140758,"version":"4.5.0"},"reference-count":63,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,2,11]],"date-time":"2022-02-11T00:00:00Z","timestamp":1644537600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Bioeng. Biotechnol."],"abstract":"<jats:p>In this study, <jats:italic>Pleurotus dryinus<\/jats:italic> was grown on municipal biosolids (BS) as the substrate to produce laccase for the removal of pesticides (fungicides, herbicides, and insecticides) from wastewater. Among the various types of BS tested, sterilized biosolids were the most promising substrate for laccase production by <jats:italic>P. dryinus<\/jats:italic> with a maximal laccase activity (162.1 \u00b1 21.1\u00a0U\/g dry substrate), followed by hygenized biosolids (96.7 \u00b1 17.6\u00a0U\/g dry substrate), unsterilized biosolids (UBS) (31.9 \u00b1 1.2\u00a0U\/g dry substrate), and alkali-treated biosolids (8.2 \u00b1 0.4\u00a0U\/g dry substrate). The ultrasound-assisted extraction of this enzyme from fermented UBS was carried out with 0.1\u00a0M phosphate buffer at pH 7.0, which increased the enzyme activity of the crude extract by 30%. To test the catalytic potential of the biocatalyst in real matrices, 1 U\/ml of recovered crude laccase extract was applied for 24\u00a0h for the removal of 29 pesticides (nine fungicides, 10 herbicides, and 10 insecticides) either separately or as a mixture from spiked biologically treated wastewater effluent. When treated with crude enzyme extract, high-priority herbicides metolachlor and atrazine were completely removed, while 93%\u201397% of the insecticides aldicarb, spinosad, and azinphos-methyl and up to 91% of kresoxim-methyl were removed. Promising results were obtained with BS-derived crude enzyme extract exhibiting improved pesticides removal, which may be due to the mediator effect resulting from the catalytic transformation of other molecules in the cocktail. The results demonstrated a promising integrated bioprocess for the removal of pesticides in wastewater using crude laccase obtained from BS.<\/jats:p>","DOI":"10.3389\/fbioe.2021.770435","type":"journal-article","created":{"date-parts":[[2022,2,11]],"date-time":"2022-02-11T08:57:20Z","timestamp":1644569840000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":33,"title":["Laccase-Driven Transformation of High Priority Pesticides Without Redox Mediators: Towards Bioremediation of Contaminated Wastewaters"],"prefix":"10.3389","volume":"9","author":[{"given":"Vasanth Kumar","family":"Vaithyanathan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vinoth Kumar","family":"Vaidyanathan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hubert","family":"Cabana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,2,11]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"224","DOI":"10.3390\/su9020224","article-title":"Solid-state Fermentation as a Novel Paradigm for Organic Waste Valorization: a Review","volume":"9","author":"Abu Yazid","year":"2017","journal-title":"Sustainability"},{"key":"B2","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1016\/s0014-5793(98)01300-3","article-title":"Oxidative Biodegradation of Phosphorothiolates by Fungal Laccase","volume":"438","author":"Amitai","year":"1998","journal-title":"FEBS Lett."},{"key":"B3","doi-asserted-by":"publisher","first-page":"119810","DOI":"10.1016\/j.jclepro.2019.119810","article-title":"Mycoremediation of Phenols and Polycyclic Aromatic Hydrocarbons from a Biorefinery Wastewater and Concomitant Production of Lignin Modifying Enzymes","volume":"253","author":"Ariste","year":"2020","journal-title":"J. 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