{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T07:20:10Z","timestamp":1776928810839,"version":"3.51.2"},"reference-count":38,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,12,22]],"date-time":"2021-12-22T00:00:00Z","timestamp":1640131200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2020YFA0907700 2018YFA0900702"],"award-info":[{"award-number":["2020YFA0907700 2018YFA0900702"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31620103901 31770098 21977067"],"award-info":[{"award-number":["31620103901 31770098 21977067"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Bioeng. Biotechnol."],"abstract":"<jats:p>Reductive aminases (RedAms) for the stereoselective amination of ketones represent an environmentally benign and economically viable alternative to transition metal\u2013catalyzed asymmetric chemical synthesis. Here, we report two RedAms from <jats:italic>Aspergillus calidoustus<\/jats:italic> (<jats:italic>Ac<\/jats:italic>RedAm) and bacteria (<jats:italic>Ba<\/jats:italic>RedAm) with NADPH-dependent features. The enzymes can synthesize a set of secondary amines using a broad range of ketone and amine substrates with up to 97% conversion. To synthesize the pharmaceutical ingredient (<jats:italic>R<\/jats:italic>)-rasagiline, we engineered <jats:italic>Ac<\/jats:italic>RedAm through rational design to obtain highly stereoselective mutants. The best mutant Q237A from <jats:italic>Ac<\/jats:italic>RedAm could synthesize (<jats:italic>R<\/jats:italic>)-rasagiline with &amp;gt;99% enantiomeric excess with moderate conversion. The features of <jats:italic>Ac<\/jats:italic>RedAm and <jats:italic>Ba<\/jats:italic>RedAm highlight their potential for further study and expand the biocatalytic toolbox for industrial applications.<\/jats:p>","DOI":"10.3389\/fbioe.2021.798147","type":"journal-article","created":{"date-parts":[[2021,12,22]],"date-time":"2021-12-22T07:44:21Z","timestamp":1640159061000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":6,"title":["Engineering of Reductive Aminases for Asymmetric Synthesis of Enantiopure Rasagiline"],"prefix":"10.3389","volume":"9","author":[{"given":"Kai","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanzhi","family":"He","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiawei","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luyao","family":"Tang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Cui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Feng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2021,12,22]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"11857","DOI":"10.1021\/acs.chemrev.9b00383","article-title":"Reductive Amination in the Synthesis of Pharmaceuticals","volume":"119","author":"Afanasyev","year":"2019","journal-title":"Chem. 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