{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T03:46:32Z","timestamp":1792122392344,"version":"4.4.0"},"reference-count":52,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,10,21]],"date-time":"2022-10-21T00:00:00Z","timestamp":1666310400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Bioeng. Biotechnol."],"abstract":"<jats:p><jats:bold>Purpose:<\/jats:bold> Spinal cord injury (SCI) has a damaging impact on patients, amid being a worldwide problem with no effective treatment. Herein, we reported a method for functional therapy of SCI in rats, wherein we combined thermo-sensitive hydrogel with Sonic Hedgehog (SHH) expressed in rat bone-marrow derived mesenchymal stem cells (RMSCs).<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Bone marrow-derived mesenchymal stem cells (BMSCs) were isolated from Sprague-Dawley (SD) female rats. The SHH was optimized and transferred into RMSCs <jats:italic>via<\/jats:italic> cationic liposomes, while thermo-sensitive hydrogel was reformed with hyaluronate (HA) and Pluronic F127. Then, a rat model with SCI was established accordingly by male SD rats and randomized into sham, model, RMSCs with hydrogel and SHH-RMSCs with hydrogel. The evaluation of SCI repair based on Basso, Beattie Bresnahanlocomotor rating scale (BBB scale) and inclined plate score. Immunofluorescence, immunohistochemistry and hematoxylin-eosin were utilized to explore the expression of protein (GFAP, GAP43, NF200 and MBP) and histopathology.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> It was demonstrated that transfection of SHH with cationic liposomes exhibited more effect in RMSCs than lipofectamine 2000. As shown in SEM, 3.5% HA-F127 demonstrated porous structure. In the MTT and dead\/live assay, 3.5% HA-F127 showed good biocompatibility for RMSCs. Both RMSCs and SHH-RMSCs groups could significantly promote BBB and inclined plate scores (<jats:italic>p<\/jats:italic> &amp;lt; 0.01) compared with the model. Furthermore, the SHH-RMSC group was significantly improved than RMSC with the expression of related proteins, where NF200, MBP, and GAP43 were principally enhanced with the GFAP expression being virtually down-regulated.<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> All in all, the results suggested that transplantation of RMSCs with SHH could improve the function of SCI and promote nerve regeneration.<\/jats:p>","DOI":"10.3389\/fbioe.2022.1001396","type":"journal-article","created":{"date-parts":[[2022,10,21]],"date-time":"2022-10-21T06:21:02Z","timestamp":1666333262000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":10,"title":["Thermo-sensitive hydrogel combined with SHH expressed RMSCs for rat spinal cord regeneration"],"prefix":"10.3389","volume":"10","author":[{"given":"Jun","family":"Gu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biao","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hajra","family":"Zafar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Chu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojun","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinjie","family":"Ni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Bao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,10,21]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"346","DOI":"10.1002\/bies.10251","article-title":"Neurofilaments and neurological disease","volume":"25","author":"Al-Chalabi","year":"2003","journal-title":"Bioessays"},{"key":"B2","doi-asserted-by":"publisher","first-page":"6380","DOI":"10.1021\/la0606704","article-title":"Thermosensitive pluronic micelles stabilized by shell cross-linking with gold nanoparticles","volume":"22","author":"Bae","year":"2006","journal-title":"Langmuir"},{"key":"B3","doi-asserted-by":"publisher","first-page":"650","DOI":"10.1021\/bm0608939","article-title":"Oil-encapsulating PEO-PPO-PEO\/PEG shell cross-linked nanocapsules for target-specific delivery of paclitaxel","volume":"8","author":"Bae","year":"2007","journal-title":"Biomacromolecules"},{"key":"B4","doi-asserted-by":"publisher","first-page":"101842","DOI":"10.1016\/j.jddst.2020.101842","article-title":"Improved oral bioavailability and target delivery of 6-shogaol via vitamin E TPGS-modified liposomes: Preparation, in-vitro and in-vivo characterizations","volume":"59","author":"Bao","year":"2020","journal-title":"J. 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