{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T17:47:23Z","timestamp":1791568043997,"version":"4.3.4"},"reference-count":77,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,10,4]],"date-time":"2022-10-04T00:00:00Z","timestamp":1664841600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Bioeng. Biotechnol."],"abstract":"<jats:p>N-acetylglucosamine (GlcNAc) is widely used in nutritional supplement and is generally produced from chitin using chitinases. While most GlcNAc is produced from colloidal chitin, it is essential that chitinases be acidic enzymes. Herein, we characterized an acidic, highly salinity tolerance and thermostable chitinase <jats:italic>Af<\/jats:italic>ChiJ, identified from the marine fungus <jats:italic>Aspergillus fumigatus<\/jats:italic> df673. Using AlphaFold2 structural prediction, a truncated \u039430<jats:italic>Af<\/jats:italic>ChiJ was heterologously expressed in <jats:italic>E. coli<\/jats:italic> and successfully purified. It was also found that it is active in colloidal chitin, with an optimal temperature of 45\u00b0C, an optimal pH of 4.0, and an optimal salt concentration of 3% NaCl. Below 45\u00b0C, it was sound over a wide pH range of 2.0\u20136.0 and maintained high activity (\u226597.96%) in 1\u20137% NaCl. A notable increase in chitinase activity was observed of \u039430<jats:italic>Af<\/jats:italic>ChiJ by the addition of Mg<jats:sup>2+<\/jats:sup>, Ba<jats:sup>2+<\/jats:sup>, urea, and chloroform. <jats:italic>Af<\/jats:italic>ChiJ first decomposed colloidal chitin to generate mainly N-acetyl chitobioase, which was successively converted to its monomer GlcNAc. This indicated that <jats:italic>Af<\/jats:italic>ChiJ is a bifunctional enzyme, composed of chitobiosidase and \u03b2-N-acetylglucosaminidase. Our result suggested that <jats:italic>Af<\/jats:italic>ChiJ likely has the potential to convert chitin-containing biomass into high-value added GlcNAc.<\/jats:p>","DOI":"10.3389\/fbioe.2022.1013313","type":"journal-article","created":{"date-parts":[[2022,10,4]],"date-time":"2022-10-04T07:53:56Z","timestamp":1664870036000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":24,"title":["Biochemical purification and characterization of a truncated acidic, thermostable chitinase from marine fungus for N-acetylglucosamine production"],"prefix":"10.3389","volume":"10","author":[{"given":"Bin","family":"He","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liyan","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dengfeng","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minguo","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengjin","family":"Ling","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hailan","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Ji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lixia","family":"Pan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,10,4]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"418","DOI":"10.1016\/j.fgb.2010.12.007","article-title":"Functional analysis of the fungal\/plant class chitinase family in Aspergillus fumigatus","volume":"48","author":"Alcazar-Fuoli","year":"2011","journal-title":"Fungal Genet. 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