{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T23:07:54Z","timestamp":1787958474352,"version":"build-2784847793"},"reference-count":38,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,11,7]],"date-time":"2022-11-07T00:00:00Z","timestamp":1667779200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Bioeng. Biotechnol."],"abstract":"<jats:p><jats:italic>Candida albicans<\/jats:italic> is an opportunistic pathogenic yeast that predominantly causes invasive candidiasis. Conventional methods for detecting <jats:italic>Candida<\/jats:italic> species are costly, take 3\u20135 days, and require skilled technicians. Rapid pathogen identification is important in managing invasive candidiasis infection. Here, a novel molecular diagnostic assay termed loop-mediated isothermal amplification combined with nanoparticles-based lateral flow biosensor (LAMP-LFB) was developed for <jats:italic>C. albicans<\/jats:italic> rapid detection<jats:italic>.<\/jats:italic> A set of six primers was designed based on the <jats:italic>C. albicans<\/jats:italic> species-specific internal transcribed spacer 2 (ITS2) gene. The <jats:italic>C. albicans<\/jats:italic>-LAMP results were visually reported by LFB within 2\u00a0min. Various fungal strains representing <jats:italic>Candida<\/jats:italic> species, as well as several Gram-negative and Gram-positive bacterial species, were used to determine the analytical sensitivity and specificity of the assay. The optimal LAMP conditions were 64 \u00b0C for 40 min, with a sensitivity of 1\u00a0fg of genomic DNA template from <jats:italic>C. albicans<\/jats:italic> pure cultures. No cross-reactions were obtained with non-<jats:italic>albicans<\/jats:italic> strains. Thus, the analytical specificity of the LAMP-LFB assay was 100%. The entire procedure could be completed within 85 min, including specimen processing (40\u00a0min), isothermal reaction (40\u00a0min), and result reporting (within 2\u00a0min). In 330 clinical samples (including 30 whole blood, 100 middle segment urine, and 200 sputum samples), all <jats:italic>C. albicans-<\/jats:italic>positive (62\/330) samples were identified by LAMP-LFB assay, and the diagnostic accuracy was 100% when compared to the traditional clinical cultural-based methods. Thus, this assay can be used as a diagnostic tool for the rapid, accurate, sensitive, low-cost and specific detection of <jats:italic>C. albicans<\/jats:italic> strains, especially in resource-limited settings.<\/jats:p>","DOI":"10.3389\/fbioe.2022.1025083","type":"journal-article","created":{"date-parts":[[2022,11,7]],"date-time":"2022-11-07T05:34:55Z","timestamp":1667799295000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":18,"title":["Establishment and application of loop-mediated isothermal amplification coupled with nanoparticle-based lateral flow biosensor (LAMP-LFB) for visual and rapid diagnosis of Candida albicans in clinical samples"],"prefix":"10.3389","volume":"10","author":[{"given":"Yu","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhong","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingrun","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Honglan","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shijun","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,11,7]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1816","DOI":"10.3201\/eid2410.180649","article-title":"Candida auris investigation workgroup Candida auris in healthcare facilities, New York, USA, 2013-2017","volume":"24","author":"Adams","year":"2018","journal-title":"Emerg. 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