{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T22:59:07Z","timestamp":1769554747971,"version":"3.49.0"},"reference-count":58,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,12,5]],"date-time":"2022-12-05T00:00:00Z","timestamp":1670198400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Bioeng. Biotechnol."],"abstract":"<jats:p>The most common primary malignant bone sarcoma is Osteogenic sarcoma (OS) which has a bimodal age distribution. Unfortunately, the treatment of OS was less effective for elderly patients than for younger ones. The study aimed to explore a new microRNA (miRNA) which can bind to combining engineered exosomes for treatment of older OS patients. Based on GSE65071 and miRNet 2.0, two up-regulated miRNAs (miR-328, miR-107) and seven down-regulated miRNAs (miR-133b, miR-206, miR-1-3p, miR-133a, miR-449a, miR-181daysay, miR-134) were selected. Next, we used FunRich software to predict the up-stream transcription factors (TFs) of differentially expressed miRNAs (DE-miRNAs). By comparing target genes predicted from DE-miRNAs with differentially expressed genes, we identified 12 down-regulated and 310 up-regulated mRNAs. For KEGG analysis, the most enriched KEGG pathway was Cell cycle, Spliceosome, and Protein digestion and absorption. By using protein-protein interactions network, topological analysis algorithm and GEPIA database, miR-449a \/CCNB1 axis was identified. Experiments <jats:italic>in vitro<\/jats:italic> were conducted to confirm the results too. MiRNA-449a is down-regulated in osteosarcoma and suppresses cell proliferation by targeting CCNB1. Our findings not only reveal a novel mechanism of miR-449a \/CCNB1 in OS but also had laid the groundwork for further investigation and analysis in the field of exosome engineering.<\/jats:p>","DOI":"10.3389\/fbioe.2022.1052252","type":"journal-article","created":{"date-parts":[[2022,12,5]],"date-time":"2022-12-05T07:21:55Z","timestamp":1670224915000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["Screening for MicroRNA combination with engineered exosomes as a new tool against osteosarcoma in elderly patients"],"prefix":"10.3389","volume":"10","author":[{"given":"Jiyu","family":"Han","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zitong","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanhong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tao","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daqian","family":"Wan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2022,12,5]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"977","DOI":"10.1002\/cam4.438","article-title":"Cross-species identification of a plasma microRNA signature for detection, therapeutic monitoring, and prognosis in osteosarcoma","volume":"4","author":"Allen-Rhoades","year":"2015","journal-title":"Cancer Med."},{"key":"B2","doi-asserted-by":"publisher","first-page":"W244","DOI":"10.1093\/nar\/gkaa467","article-title":"miRNet 2.0: network-based visual analytics for miRNA functional analysis and systems biology","volume":"48","author":"Chang","year":"2020","journal-title":"Nucleic Acids Res."},{"key":"B3","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1016\/j.tig.2018.12.005","article-title":"miRNA targeting: Growing beyond the seed","volume":"35","author":"Chipman","year":"2019","journal-title":"Trends Genet."},{"key":"B4","doi-asserted-by":"publisher","first-page":"153642","DOI":"10.1016\/j.phymed.2021.153642","article-title":"Dihydromyricetin suppresses cell metastasis in human osteosarcoma through SP-1- and NF-\u03baB-modulated urokinase plasminogen activator inhibition","volume":"90","author":"Chou","year":"2021","journal-title":"Phytomedicine"},{"key":"B5","doi-asserted-by":"publisher","first-page":"178","DOI":"10.1186\/s13046-020-01685-w","article-title":"The role of extracelluar matrix in osteosarcoma progression and metastasis","volume":"39","author":"Cui","year":"2020","journal-title":"J. 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