{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T00:53:32Z","timestamp":1768265612436,"version":"3.49.0"},"reference-count":33,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,3,9]],"date-time":"2022-03-09T00:00:00Z","timestamp":1646784000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004281","name":"Narodowe Centrum Nauki","doi-asserted-by":"publisher","award":["2018\/31\/B\/ST8\/03170"],"award-info":[{"award-number":["2018\/31\/B\/ST8\/03170"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Bioeng. Biotechnol."],"abstract":"<jats:p><jats:italic>Pseudomonas aeruginosa<\/jats:italic> is a producer of desired secondary metabolites, including pyocyanin. Potential uses of this pigment urge a search for improved production methods. Recent trends in bioprocessing show the potential of the use of electromagnetic fields (EMFs) to influence the growth of microorganisms and even modulate the concentration of bioproducts. Here, we aimed at assessing the influence of rotating magnetic field (RMF) and static magnetic field (SMF) on pyocyanin production, growth rate, and respiration of <jats:italic>P. aeruginosa.<\/jats:italic> Moreover, exposure time to EMFs (2, 6, and 12\u00a0h) and culture volume (10 and 50\u00a0ml) were initially assessed. <jats:italic>P. aeruginosa<\/jats:italic> was cultivated in magnetically assisted reactors with 5 and 50\u00a0Hz RMF (magnetic induction of 24.32 and 42.64\u00a0mT, respectively) and SMF (\u221217.37\u00a0mT). Growth kinetics was assessed with Gompertz equation. The viability was tested using resazurin assay, whereas pyocyanin production by chloroform-HCl methodology. The growth of <jats:italic>P. aeruginosa<\/jats:italic> was slightly stimulated by exposure to a RMF with 50\u00a0Hz (108% related to the control) and significantly by SMF (132% related to the control), while RMF 5\u00a0Hz exposure prolonged the time of inflection (in comparison to RMF 50\u00a0Hz and SMF). The 6-h exposure to EMFs resulted in the highest pyocyanin production in comparison to the control, indicating a relationship between exposure time and product concentration. Moreover, cultures led in smaller volumes produced more pyocyanin. Our findings show that the use of different EMF types, frequency, and exposition time and volume could be used interchangeably to obtain different bioprocess aims.<\/jats:p>","DOI":"10.3389\/fbioe.2022.795871","type":"journal-article","created":{"date-parts":[[2022,3,9]],"date-time":"2022-03-09T10:50:02Z","timestamp":1646823002000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":8,"title":["Application of Magnetically Assisted Reactors for Modulation of Growth and Pyocyanin Production by Pseudomonas aeruginosa"],"prefix":"10.3389","volume":"10","author":[{"given":"Joanna","family":"Jab\u0142o\u0144ska","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kamila","family":"Dubrowska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adrian","family":"Augustyniak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marian","family":"Kordas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafa\u0142","family":"Rakoczy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2022,3,9]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"a","DOI":"10.1002\/BEM.20523","article-title":"Exposure to High Static or Pulsed Magnetic fields Does Not Affect Cellular Processes in the yeastSaccharomyces Cerevisiae","volume":"31","author":"Anton-Leberre","year":"2009","journal-title":"Bioelectromagnetics"},{"key":"B2","doi-asserted-by":"publisher","first-page":"1","DOI":"10.7573\/dic.212527","article-title":"How to Manage Pseudomonas aeruginosa Infections","volume":"7","author":"Bassetti","year":"2018","journal-title":"Dic"},{"key":"B3","doi-asserted-by":"publisher","first-page":"5299","DOI":"10.3390\/app11115299","article-title":"Static Magnetic Fields Effects on Polysaccharides Production by Different Microalgae Strains","volume":"11","author":"Deamici","year":"2021","journal-title":"Appl. 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