{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T02:04:03Z","timestamp":1789610643592,"version":"build-2803163510"},"reference-count":35,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,3,8]],"date-time":"2022-03-08T00:00:00Z","timestamp":1646697600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Bioeng. Biotechnol."],"abstract":"<jats:p>In the current study, we synthesized nanocellulose (NCF)-collagen (Col)-nano hydroxyapatite (NHA) organic-inorganic hybrid aerogels loaded with stromal cell derived factor-1 (SDF-1) and sclerostin monoclonal antibody (SOST McAb) and investigated their ability to repair steroid-induced osteonecrosis. Rabbit bone marrow mesenchymal stem cells (BMSCs) and human vascular endothelial cells (HUVECs) were used for the <jats:italic>in vitro<\/jats:italic> study. A rabbit steroid-induced osteonecrosis model was used for the <jats:italic>in vivo<\/jats:italic> study. The best elastic modulus reached 12.95 \u00b1 4.77\u00a0MPa with a mean compressive property of 0.4067 \u00b1 0.084\u00a0MPa for the scaffold containing 100% mass fraction. The average pore diameter of the aerogel was 75 \u00b1 18\u00a0\u00b5m with a porosity of more than 90% (96.4 \u00b1 1.6%). The aerogel-loaded SDF-1 and SOST were released at 40\u201350% from the material within the initial 3\u00a0h and maintained a stable release for more than 21\u00a0days. The <jats:italic>in vitro<\/jats:italic> study showed osteogenesis and vascularization capabilities of the scaffold. The <jats:italic>in vivo<\/jats:italic> study showed that rabbits received implantation of the scaffold with SOST McAb and SDF-1 showed the best osteogenesis of the osteonecrosis zone in the femoral head. Imaging examination revealed that most of the necrotic area of the femoral head was repaired. These results suggest that this hybrid aerogel scaffold could be used for future steroid-induced osteonecrosis repair.<\/jats:p>","DOI":"10.3389\/fbioe.2022.825231","type":"journal-article","created":{"date-parts":[[2022,3,8]],"date-time":"2022-03-08T10:04:26Z","timestamp":1646733866000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":8,"title":["In vitro and in vivo Repair Effects of the NCF-Col-NHA Aerogel Scaffold Loaded With SOST Monoclonal Antibody and SDF-1 in Steroid-Induced Osteonecrosis"],"prefix":"10.3389","volume":"10","author":[{"given":"Bing","family":"Xu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeyu","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeyu","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zongke","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haoyang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,3,8]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"726","DOI":"10.3390\/polym11040726","article-title":"Nanomaterials in Advanced, High-Performance Aerogel Composites: A Review","volume":"11","author":"Barrios","year":"2019","journal-title":"Polymers (Basel)"},{"key":"B2","doi-asserted-by":"publisher","first-page":"2021","DOI":"10.2147\/ijn.s124318","article-title":"Cellulose Nanofiber Aerogel as a Promising Biomaterial for Customized Oral Drug Delivery","author":"Bhandari","year":"2017","journal-title":"Ijn"},{"key":"B3","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1186\/s13036-020-0232-x","article-title":"The Long-Term Residual Effects of Low-Magnitude Mechanical Stimulation Therapy on Skeletal Health","volume":"14","author":"Bodnyk","year":"2020","journal-title":"J. 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