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Biotechnol."],"abstract":"<jats:p>Prosthetic implants of expanded polytetrafluoroethylene (ePTFE) in the cardiovascular system have a high failure rate over the long term because of thrombosis and intimal hyperplasia. Although multiple surface modification methods have been applied to improve the anti-thrombotic and <jats:italic>in situ<\/jats:italic> endothelialization abilities of ePTFE, none have delivered outstanding results <jats:italic>in vivo<\/jats:italic>. Our previous study combined heparin\/collagen multilayers and REDV peptides to modify ePTFE, and the <jats:italic>in-vitro<\/jats:italic> results showed that modification ePTFE with heparin\/collagen-REDV can promote the cytocompatibility and antiplatelet property. This study illustrated the physical change, selective endothelial cells capture ability, and <jats:italic>in vivo<\/jats:italic> performance in further. The physical test demonstrated that this modification improved the hydrophilicity, flexibility and strength of ePTFE. A competition experiment of co-cultured endothelial cells and vascular smooth muscle cells verified that the heparin\/collagen-REDV modification had high specificity for endothelial cell capture. A rabbit animal model was constructed to evaluate the <jats:italic>in vivo<\/jats:italic> performance of modified ePTFE implanted in the right ventricular outflow tract. The results showed that heparin\/collagen-REDV modification was safe, promoted endothelialization, and successfully achieved regional anti-thrombosis without influencing body-wide coagulation function. The pathologic manifestations and mRNA expression pattern in tissues in contact with modified ePTFE indicated that this modification method may reduce M2-type macrophage infiltration and the expression of genes related to immune and inflammatory responses. The heparin\/collagen-REDV modification may lower the incidence of complications related to ePTFE implantation and has good prospects for clinical use.<\/jats:p>","DOI":"10.3389\/fbioe.2022.916931","type":"journal-article","created":{"date-parts":[[2022,8,4]],"date-time":"2022-08-04T05:10:49Z","timestamp":1659589849000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":5,"title":["Heparin\/Collagen-REDV Modification of Expanded Polytetrafluoroethylene Improves Regional Anti-thrombosis and Reduces Foreign Body Reactions in Local Tissues"],"prefix":"10.3389","volume":"10","author":[{"given":"Yaping","family":"Shan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiqi","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaxi","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaping","family":"Mi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenbo","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huifeng","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing","family":"Jia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,8,4]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"Cd001487","DOI":"10.1002\/14651858.CD001487.pub3","article-title":"Graft Type for Femoro-Popliteal Bypass Surgery","volume":"2018","author":"Ambler","year":"2018","journal-title":"Cochrane Database Syst. 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