{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T18:50:22Z","timestamp":1785523822584,"version":"3.56.0"},"reference-count":44,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,9,12]],"date-time":"2022-09-12T00:00:00Z","timestamp":1662940800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Bioeng. Biotechnol."],"abstract":"<jats:p>The developmental stage of the mosquito is one of the main factors that affect its response to ionizing radiation. Irradiation of adults has been reported to have beneficial effects. However, the main challenge is to immobilize and compact a large number of adult male mosquitoes for homogenous irradiation with minimal deleterious effects on their quality. The present study investigates the use of nitrogen in the irradiation of adult <jats:italic>Aedes albopictus<\/jats:italic> and <jats:italic>Ae. aegypti<\/jats:italic>. Irradiation in nitrogen (N<jats:sub>2<\/jats:sub>) and in air after being treated with nitrogen (PreN<jats:sub>2<\/jats:sub>) were compared with irradiation in air at gamma radiation doses of 0, 55, 70, 90, 110, and 125\u00a0Gy. In both species, approximately 0% egg hatch rate was observed following doses above 55\u00a0Gy in air versus 70\u00a0Gy in PreN<jats:sub>2<\/jats:sub> and 90\u00a0Gy in N<jats:sub>2<\/jats:sub>. Males irradiated at a high mosquito density showed similar egg hatch rates as those irradiated at a low density. Nitrogen treatments showed beneficial effects on the longevity of irradiated males for a given dose, revealing the radioprotective effect of anoxia. However, irradiation in N<jats:sub>2<\/jats:sub> or PreN<jats:sub>2<\/jats:sub> slightly reduced the male flight ability. Nitrogen treatment was found to be a reliable method for adult mosquito immobilization. Overall, our results demonstrated that nitrogen may be useful in adult <jats:italic>Aedes<\/jats:italic> mass irradiation. The best option seems to be PreN<jats:sub>2<\/jats:sub> since it reduces the immobilization duration and requires a lower dose than that required in the N<jats:sub>2<\/jats:sub> environment to achieve full sterility but with similar effects on male quality. However, further studies are necessary to develop standardized procedures including containers, time and pressure for flushing with nitrogen, immobilization duration considering mosquito species, age, and density.<\/jats:p>","DOI":"10.3389\/fbioe.2022.942654","type":"journal-article","created":{"date-parts":[[2022,9,12]],"date-time":"2022-09-12T06:21:31Z","timestamp":1662963691000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":10,"title":["Response of male adult Aedes mosquitoes to gamma radiation in different nitrogen environments"],"prefix":"10.3389","volume":"10","author":[{"given":"Nanwintoum S\u00e9verin","family":"Bimbil\u00e9 Somda","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanano","family":"Yamada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carina","family":"Kraupa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wadaka","family":"Mamai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamidou","family":"Maiga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simran Singh","family":"Kotla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Wallner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Martina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeremy","family":"Bouyer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,9,12]]},"reference":[{"key":"B1","first-page":"22","article-title":"Guidelines for mark-release-recapture procedures of Aedes mosquitoes. 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