{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T06:52:00Z","timestamp":1787986320546,"version":"build-2784847793"},"reference-count":22,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,2,7]],"date-time":"2023-02-07T00:00:00Z","timestamp":1675728000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Bioeng. Biotechnol."],"abstract":"<jats:p>Tuberculosis (TB) poses a serious threat to human health and social development. Accurate diagnosis of <jats:italic>mycobacterium tuberculosis<\/jats:italic> infection plays a critical role in the prevention and treatment of tuberculosis. Interferon-\u03b3 (INF-\u03b3) release assay (IGRA) is currently the only quantitative tuberculosis infection diagnosis method. An accurate, fast, and easily handled INF-\u03b3 detection method is the key to obtaining accurate results. Herein, we report a novel paper-based electrochemiluminescence (ECL) method based on lateral flow immunosensors that combines the easy handling characteristics of immunochromatography and the high sensitivity of electrochemiluminescence to detect IFN-\u03b3. To our knowledge this is the first INF-\u03b3 detection method that combines immunochromatography with electrochemiluminescence. The paper-based ECL-LFI test consists of a sample pad, conjugation pad (with binding antibody IFN-\u03b3-Ab1 conjugated with ruthenium tripyridine), detection pad (with capture antibody IFN-\u03b3-Ab2 immobilized on nanospheres), absorbent pad, and electrode for signal activation. The ECL signal is obtained by cyclic voltammetry scanning at a speed of 0.1\u00a0V\/s in the detection area of the paper-based ECL-LFI test. In our experiments, the paper-based ECL-LFI test exhibited a minimum detection limit of 2.57\u00a0pg\/mL within 12 min, and a broad detection range of 2.57\u20135,000\u00a0pg\/mL, with repeatability of 8.10% and stability of 4.97%. With the advantage of high accuracy and sensitivity, easy handling, and low user training requirements, this ECL-LFI test might be used as point-of-care testing (POCT) in the IGRA for tuberculosis diagnosis.<\/jats:p>","DOI":"10.3389\/fbioe.2023.1131840","type":"journal-article","created":{"date-parts":[[2023,2,7]],"date-time":"2023-02-07T05:22:05Z","timestamp":1675747325000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":15,"title":["Portable paper-based electrochemiluminescence test incorporating lateral-flow immunosensors for detection of interferon-\u03b3 levels"],"prefix":"10.3389","volume":"11","author":[{"given":"Shichao","family":"Yuan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guihua","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongbin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,2,7]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"296","DOI":"10.1007\/s00604-021-04959-y","article-title":"Electrochemiluminescence paper-based screen-printed electrode for HbA1c detection using two-dimensional zirconium metal-organic framework\/Fe3O4 nanosheet composites decorated with Au nanoclusters","volume":"188","author":"Ahmadi","year":"2021","journal-title":"Microchim. 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