{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T13:34:02Z","timestamp":1791120842124,"version":"4.1.0"},"reference-count":34,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,6,5]],"date-time":"2023-06-05T00:00:00Z","timestamp":1685923200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Bioeng. Biotechnol."],"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Tissue welding is an electrosurgical technique that can fuse tissue for small intestine anastomosis. However, limited knowledge exists on its application in mucosa-mucosa end-to-end anastomosis. This study investigates the effects of initial compression pressure, out-put power, and duration time on anastomosis strength <jats:italic>ex vivo<\/jats:italic> in mucosa-mucosa end-to-end anastomosis.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold><jats:italic>Ex vivo<\/jats:italic> porcine bowel segments were used to create 140 mucosa-mucosa end-to-end fusions. Different experimental parameters were employed for fusion, including initial com-pression pressure (50kPa\u2013400\u00a0kPa), output power (90W, 110W, and 140W), and fusion time (5, 10, 15, 20\u00a0s). The fusion quality was measured by burst pressure and optical microscopes.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> The best fusion quality was achieved with an initial compressive pressure between 200 and 250\u00a0kPa, an output power of 140W, and a fusion time of 15\u00a0s. However, an increase in output power and duration time resulted in a wider range of thermal damage. There was no significant difference between the burst pressure at 15 and 20\u00a0s (<jats:italic>p<\/jats:italic> &amp;gt; 0.05). However, a substantial increase in thermal damage was observed with longer fusion times of 15 and 20\u00a0s (<jats:italic>p<\/jats:italic> &amp;lt; 0.05).<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> The best fusion quality for mucosa-mucosa end-to-end anastomosis <jats:italic>ex vivo<\/jats:italic> is achieved when the initial compressive pressure is between 200 and 250\u00a0kPa, the output power is approximately 140W, and the fusion time is approximately 15\u00a0s. These findings can serve as a valuable theoretical foundation and technical guidance for conducting animal experiments <jats:italic>in vivo<\/jats:italic> and subsequent tissue regeneration.<\/jats:p>","DOI":"10.3389\/fbioe.2023.1200239","type":"journal-article","created":{"date-parts":[[2023,6,5]],"date-time":"2023-06-05T05:12:16Z","timestamp":1685941936000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":9,"title":["An ex vivo preliminary investigation into the impact of parameters on tissue welding strength in small intestine mucosa-mucosa end-to-end anastomosis"],"prefix":"10.3389","volume":"11","author":[{"given":"Caihui","family":"Zhu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Yin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianzhi","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haotian","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaowei","family":"Xiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Qiu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kefu","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,6,5]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"3485","DOI":"10.1007\/s00464-013-2935-2","article-title":"Radiofrequency-induced small bowel thermofusion: An ex vivo study of intestinal seal adequacy using mechanical and imaging modalities","volume":"27","author":"Arya","year":"2013","journal-title":"Surg. 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