{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T11:24:10Z","timestamp":1769167450777,"version":"3.49.0"},"reference-count":46,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,7,28]],"date-time":"2023-07-28T00:00:00Z","timestamp":1690502400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Bioeng. Biotechnol."],"abstract":"<jats:p>Current leading managements for diverticular disease cannot prevent the recurrence of diverticulitis, bleeding and\/or other complications. There is an immediate need for developing new minimal invasive therapeutic strategies to prevent and treat this disease. Through a biomechanical analysis of porcine colon with diverticular lesions, we proposed a novel adhesive patch concept aiming at mechanical reconstruction of the diseased colon wall. This study aims to evaluate the surgical feasibility (safety and efficacy) of pulmonary visceral pleura (PVP) patch therapy using a pig model of diverticulosis. Six female Yucatan miniature pigs underwent collagenase injection (CI) for the development of diverticular lesions. The lesions in each animal either received patch implantation (treated group, <jats:italic>n<\/jats:italic> = 40 for 6 pigs) or left intact (untreated group, <jats:italic>n<\/jats:italic> = 44 for 6 pigs). The normal colonic wall in each animal received patch implantation at two spots to serve as control (n = 12 for 6 pigs). After 3\u00a0months of observation, the performance and safety of the patch treatment were evaluated through macroscopic and histological examination. We found that 95% of pouch-like herniation of the mucosa was prevented from the colon wall with the treatment. The pouch diameter was significantly reduced in the treated group as compared to the untreated group (<jats:italic>p<\/jats:italic> &amp;lt; 0.001). The patch application caused a significant increase in the levels of collagen of the colon tissue as compared to the untreated and control groups (<jats:italic>p<\/jats:italic> &amp;lt; 0.001). No difference was found in the lymphocyte and macrophage inflammatory infiltrate between the groups. Our results suggest that patch treatment efficiently inhibits the diverticular pouch deformation and promotes the healing of the colon wall with a normal inflammatory response, which may minimize the risk of diverticulosis reoccurrence and complications over time.<\/jats:p>","DOI":"10.3389\/fbioe.2023.1215362","type":"journal-article","created":{"date-parts":[[2023,7,31]],"date-time":"2023-07-31T21:47:11Z","timestamp":1690840031000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Novel patch biomaterial treatment for colon diverticulosis in swine model"],"prefix":"10.3389","volume":"11","author":[{"given":"Xiaomei","family":"Guo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bhavesh","family":"Patel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling","family":"Han","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William G.","family":"Van Alstine","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jillian N.","family":"Noblet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sean D.","family":"Chambers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ghassan S.","family":"Kassab","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2023,7,28]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"178","DOI":"10.4103\/0974-2077.123395","article-title":"Cutaneous wound closure materials: An overview and update","volume":"6","author":"Al-Mubarak","year":"2013","journal-title":"J. Cutan. Aesthet. Surg."},{"key":"B2","doi-asserted-by":"publisher","first-page":"219","DOI":"10.4103\/1117-1936.169543","article-title":"Spectrum of colonoscopy findings in Ile-Ife Nigeria","volume":"19","author":"Alatise","year":"2012","journal-title":"Niger. Postgrad. Med. J."},{"key":"B3","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1016\/S1072-7515(99)00068-X","article-title":"Use of N-butyl-2-cyanoacrylate in elective surgical incisions\u2014Longterm outcomes11No competing interests declared","volume":"189","author":"Amiel","year":"1999","journal-title":"J. Am. Coll. Surg."},{"key":"B4","doi-asserted-by":"publisher","first-page":"490","DOI":"10.1007\/s11426-014-5086-y","article-title":"Synthetic biodegradable functional polymers for tissue engineering: A brief review","volume":"57","author":"BaoLin","year":"2014","journal-title":"Sci. China Chem."},{"key":"B5","doi-asserted-by":"publisher","first-page":"716","DOI":"10.1016\/j.transproceed.2017.02.017","article-title":"Electrospun PHEA-PLA\/PCL scaffold for vascular regeneration: A preliminary in vivo evaluation","volume":"49","author":"Buscemi","year":"2017","journal-title":"Transpl. Proc."},{"key":"B6","first-page":"14904","article-title":"A convenient method for quantifying collagen fibers in atherosclerotic lesions by ImageJ software","volume":"10","author":"Chen","year":"2017","journal-title":"Int. J. Clin. Exp. Med."},{"key":"B7","doi-asserted-by":"publisher","first-page":"19001","DOI":"10.1021\/la104206h","article-title":"Three-dimensional scaffolds for tissue engineering: The importance of uniformity in pore size and structure","volume":"26","author":"Choi","year":"2010","journal-title":"Langmuir"},{"key":"B8","doi-asserted-by":"publisher","first-page":"e1710","DOI":"10.1097\/MD.0000000000001710","article-title":"New trends in acute management of colonic diverticular bleeding: A systematic review","volume":"94","author":"Cirocchi","year":"2015","journal-title":"Medicine"},{"key":"B9","first-page":"24","article-title":"Recent advances in the treatment of colonic diverticular disease and prevention of acute diverticulitis","volume":"29","author":"Elisei","year":"2016","journal-title":"Ann. Gastroenterol."},{"key":"B10","doi-asserted-by":"publisher","first-page":"290","DOI":"10.1007\/s00464-003-9032-x","article-title":"Experience with the use of tissue adhesives in pediatric endoscopic surgery","volume":"18","author":"Esposito","year":"2004","journal-title":"Surg. Endosc."},{"key":"B11","doi-asserted-by":"publisher","first-page":"312","DOI":"10.1111\/j.1463-1318.2009.02121.x","article-title":"The changing trend of diverticular disease in a developing nation","volume":"13","author":"Fong","year":"2011","journal-title":"Colorectal. Dis."},{"key":"B12","doi-asserted-by":"publisher","first-page":"333","DOI":"10.1007\/s11894-013-0333-5","article-title":"Lower GI bleeding: Epidemiology and management","volume":"15","author":"Ghassemi","year":"2013","journal-title":"Curr. Gastroenterol. Rep."},{"key":"B13","doi-asserted-by":"publisher","first-page":"2149","DOI":"10.1007\/s00384-022-04248-x","article-title":"Recurrent diverticulitis after elective surgery","volume":"37","author":"Giulio","year":"2022","journal-title":"Int. J. Colorectal Dis."},{"key":"B14","doi-asserted-by":"publisher","first-page":"G51","DOI":"10.1152\/ajpgi.00408.2018","article-title":"Novel swine model of colonic diverticulosis","volume":"317","author":"Guo","year":"2019","journal-title":"Am. J. Physiol. Gastr. L."},{"key":"B15","doi-asserted-by":"publisher","first-page":"100862","DOI":"10.1016\/j.cpsurg.2020.100862","article-title":"Diverticulitis: An update from the age old paradigm","volume":"57","author":"Hawkins","year":"2020","journal-title":"Curr. Probl. Surg."},{"key":"B16","doi-asserted-by":"publisher","first-page":"4441","DOI":"10.3748\/wjg.v27.i27.4441","article-title":"Recent trends in the prevalence and distribution of colonic diverticula in Japan evaluated using computed tomography colonography","volume":"27","author":"Isohata","year":"2021","journal-title":"World J. Gastroenterol."},{"key":"B17","doi-asserted-by":"publisher","first-page":"409","DOI":"10.1097\/00002480-200007000-00007","article-title":"Use of a new elastin patch and glue for repair of a major duodenal injury","volume":"46","author":"Kajitani","year":"2000","journal-title":"ASAIO. J."},{"key":"B18","doi-asserted-by":"publisher","first-page":"663","DOI":"10.1007\/s10620-015-3924-1","article-title":"Minimally invasive management of complicated diverticular disease: Current status and review of literature","volume":"61","author":"Kaushik","year":"2016","journal-title":"Dig. Dis. Sci."},{"key":"B19","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1186\/1754-9493-4-5","article-title":"Complications in colorectal surgery: Risk factors and preventive strategies","volume":"4","author":"Kirchhoff","year":"2010","journal-title":"Patient. Saf. Surg."},{"key":"B20","doi-asserted-by":"publisher","first-page":"225","DOI":"10.4161\/org.6.4.12555","article-title":"Tissue repair: The hidden drama","volume":"6","author":"Krafts","year":"2010","journal-title":"Organogenesis"},{"key":"B21","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1007\/s10029-011-0887-9","article-title":"Efficiency and safety of mesh fixation in laparoscopic inguinal hernia repair using n-butyl cyanoacrylate: Long-term biocompatibility in over 1,300 mesh fixations","volume":"16","author":"Kukleta","year":"2012","journal-title":"Hernia"},{"key":"B22","first-page":"841","article-title":"Biocompatibility and biodegradability of electrospun PHEA-PLA scaffolds: Our preliminary experience in a murine animal model","volume":"7","author":"Lo Monte","year":"2012","journal-title":"Dig. J. Nanomat Biostruct"},{"key":"B23","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1007\/s00384-020-03762-0","article-title":"Surgical resection does not avoid the risk of diverticulitis recurrence-a systematic review of risk factors","volume":"36","author":"Longchamp","year":"2021","journal-title":"Int. J. Colorectal Dis."},{"key":"B24","first-page":"55","article-title":"A morphologic study of the submucosa of the large intestine","volume":"145","author":"Lord","year":"1977","journal-title":"Surg. Gyneco.l Obstet."},{"key":"B25","doi-asserted-by":"publisher","first-page":"458","DOI":"10.1016\/j.jvsv.2019.09.011","article-title":"Homologous and heterologous assessment of a novel biomaterial for venous patch","volume":"8","author":"Lu","year":"2020","journal-title":"J. Vasc. Surg. Venous. Lymphat. Disord."},{"key":"B26","doi-asserted-by":"publisher","first-page":"679466","DOI":"10.3389\/fbioe.2021.679466","article-title":"Novel biomaterial for artery patch in swine model with high-fat diet","volume":"9","author":"Lu","year":"2021","journal-title":"Front. Bioeng. Biotechnol."},{"key":"B27","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1016\/j.actbio.2018.11.001","article-title":"In vivo self-assembly of small diameter pulmonary visceral pleura artery graft","volume":"83","author":"Lu","year":"2019","journal-title":"Acta. Biomater."},{"key":"B28","doi-asserted-by":"publisher","first-page":"796076","DOI":"10.3389\/fbioe.2022.796076","article-title":"Pulmonary visceral pleura biomaterial: Elastin- and collagen-based extracellular matrix","volume":"10","author":"Lu","year":"2022","journal-title":"Front. Bioeng. Biotechnol."},{"key":"B29","doi-asserted-by":"publisher","first-page":"1867","DOI":"10.1016\/j.jpedsurg.2004.08.018","article-title":"Laparoscopic injection of dermabond tissue adhesive for the repair of inguinal hernia: Short- and long-term follow-up","volume":"39","author":"Miyano","year":"2004","journal-title":"J. Pediatr. Surg."},{"key":"B30","doi-asserted-by":"publisher","first-page":"6014","DOI":"10.1038\/s41598-020-63049-w","article-title":"Computational analysis of mechanical stress in colonic diverticulosis","volume":"10","author":"Patel","year":"2020","journal-title":"Sci. Rep."},{"key":"B31","doi-asserted-by":"publisher","first-page":"1409","DOI":"10.1007\/s10620-018-5071-y","article-title":"Animal models of diverticulosis: Review and recommendations","volume":"63","author":"Patel","year":"2018","journal-title":"Di.g Di.s Sci."},{"key":"B32","doi-asserted-by":"publisher","first-page":"980","DOI":"10.1016\/j.cgh.2016.01.020","article-title":"Distribution and characteristics of colonic diverticula in a United States screening population","volume":"14","author":"Peery","year":"2016","journal-title":"Clin. Gastroenterol. Hepatol."},{"key":"B33","doi-asserted-by":"publisher","first-page":"29","DOI":"10.3390\/medicina58010029","article-title":"Conservative management of complicated colonic diverticulitis in early and late elderly","volume":"58","author":"Perrone","year":"2021","journal-title":"Med."},{"key":"B34","doi-asserted-by":"publisher","first-page":"913","DOI":"10.1007\/s10620-022-07600-x","article-title":"Gut microbiota association with diverticular disease pathogenesis and progression: A systematic review","volume":"68","author":"Reitano","year":"2023","journal-title":"Dig. Dis. Sci."},{"key":"B35","doi-asserted-by":"publisher","first-page":"286ra68","DOI":"10.1126\/scitranslmed.3010658","article-title":"Collagen degradation and MMP9 activation byEnterococcus faecaliscontribute to intestinal anastomotic leak","volume":"7","author":"Shogan","year":"2015","journal-title":"Sci. Transl. Med."},{"key":"B36","doi-asserted-by":"publisher","first-page":"1282","DOI":"10.1053\/j.gastro.2018.12.033","article-title":"Epidemiology, pathophysiology, and treatment of diverticulitis","volume":"156","author":"Strate","year":"2019","journal-title":"Gastroenterology"},{"key":"B37","doi-asserted-by":"publisher","first-page":"340","DOI":"10.1039\/c7bm00545h","article-title":"Enhanced wound healing in diabetic rats by nanofibrous scaffolds mimicking the basketweave pattern of collagen fibrils in native skin","volume":"6","author":"Sun","year":"2018","journal-title":"Biomater. Sci."},{"key":"B38","doi-asserted-by":"publisher","first-page":"248","DOI":"10.1007\/s11427-013-4603-0","article-title":"Tissue engineered esophagus by copper-small intestinal submucosa graft for esophageal repair in a canine model","volume":"57","author":"Tan","year":"2014","journal-title":"Sci. China. Life. Sci."},{"key":"B39","doi-asserted-by":"publisher","first-page":"1555","DOI":"10.1007\/s10856-011-4316-5","article-title":"Fabrication of nano-structured electrospun collagen scaffold intended for nerve tissue engineering","volume":"22","author":"Timnak","year":"2011","journal-title":"J. Mater Sci. Mater Med."},{"key":"B40","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1002\/ags3.12035","article-title":"Management of complicated diverticulitis of the colon","volume":"2","author":"Tochigi","year":"2017","journal-title":"Ann. Gastroenterol. Surg."},{"key":"B41","doi-asserted-by":"publisher","first-page":"664","DOI":"10.1111\/apt.13322","article-title":"Review article: The pathophysiology and medical management of diverticulosis and diverticular disease of the colon","volume":"42","author":"Tursi","year":"2015","journal-title":"Aliment. Pharmacol. Ther."},{"key":"B42","doi-asserted-by":"publisher","first-page":"961","DOI":"10.1007\/s00384-017-2834-4","article-title":"Impact of tissue adhesives on the prevention of anastomotic leakage of colonic anastomoses: An in vivo study","volume":"32","author":"Vakalopoulos","year":"","journal-title":"Int. J. Colorectal. Dis."},{"key":"B43","doi-asserted-by":"publisher","first-page":"846","DOI":"10.1002\/jbm.b.33621","article-title":"Clinical, mechanical, and immunohistopathological effects of tissue adhesives on the colon: An in-vivo study: Effects of Tissue Adhesives on the Colon: An in Vivo Study","volume":"105","author":"Vakalopoulos","year":"","journal-title":"J. Biomed. Mater. Res. B. Appl. Biomater."},{"key":"B44","doi-asserted-by":"publisher","first-page":"157","DOI":"10.1007\/s00384-023-04454-1","article-title":"Incidence of diverticulitis recurrence after sigmoid colectomy: A retrospective cohort study from a tertiary center and systematic review","volume":"38","author":"Waser","year":"2023","journal-title":"Int. J. Colorectal Dis."},{"key":"B45","doi-asserted-by":"publisher","first-page":"203","DOI":"10.1159\/000450683","article-title":"Laparoscopic versus open surgery for diverticulitis: A systematic review and meta-analysis","volume":"34","author":"Wu","year":"2017","journal-title":"Dig. Surg."},{"key":"B46","doi-asserted-by":"publisher","first-page":"721","DOI":"10.1111\/den.12534","article-title":"Endoscopic management of colonic diverticular bleeding","volume":"27","author":"Yamada","year":"2015","journal-title":"Dig. Endosc."}],"container-title":["Frontiers in Bioengineering and Biotechnology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fbioe.2023.1215362\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,31]],"date-time":"2023-07-31T21:47:16Z","timestamp":1690840036000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fbioe.2023.1215362\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7,28]]},"references-count":46,"alternative-id":["10.3389\/fbioe.2023.1215362"],"URL":"https:\/\/doi.org\/10.3389\/fbioe.2023.1215362","relation":{},"ISSN":["2296-4185"],"issn-type":[{"value":"2296-4185","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,7,28]]},"article-number":"1215362"}}