{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T17:14:17Z","timestamp":1793294057358,"version":"4.5.0"},"reference-count":29,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,8,3]],"date-time":"2023-08-03T00:00:00Z","timestamp":1691020800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Bioeng. Biotechnol."],"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Tongue cancer is the most common type of oral cancer, and patients have a poor prognosis and quality of life after conventional surgical treatment. Honokiol (HNK) is a kind of lignan extracted from Chinese herbal medicine Houpu, many domestic and international experiments have demonstrated its anti-tumor effect. Titanium dioxide nanotube (TNTs) is a kind of nanomaterial which can be used as drug carrier. The purpose of this study is to explore the effects of HNK-loaded TNTs delivery system (HNK-TNTs) on anti-tumor.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> TNTs were prepared by anodic oxidation method, and HNK was loaded onto TNTs by physical adsorption. The effect of HNK-TNTs on the proliferation, migration and apoptosis of CAL-27 cells were explored by CCK-8 experiment, scratch assay, live and dead staining and cellular immunofluorescence analysis.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> The material characterization test results showed that we had successfully prepared HNK-TNTs. CCK-8 experiment, scratch assay showed that the proliferation and migration ability of CAL-27 cells were significantly weakened after treatment with HNK-TNTs, and their cell proliferation rates significantly decreased. Live\/dead staining, cell immunofluorescence analysis showed that HNK-TNTs could promote CAL-27 cells apoptosis by increasing the expression levels of the apoptosis-related protein Bax and Fas. Conclusion: In this experiment, we had successfully prepared Honokiol-loaded titanium dioxide nanotube drug delivery system (HNK-TNTs) and compared the effects of single drug HNK and HNK-TNTs on the proliferation, apoptosis and migration of tongue cancer CAL-27 cells. This experiment showed that HNK-TNTs had greater anti-proliferative, apoptosis-promoting and migration-inhibiting effects than the HNK as a single drug.<\/jats:p>","DOI":"10.3389\/fbioe.2023.1249349","type":"journal-article","created":{"date-parts":[[2023,8,3]],"date-time":"2023-08-03T16:47:12Z","timestamp":1691081232000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":6,"title":["Preparation of honokiol-loaded titanium dioxide nanotube drug delivery system and its effect on CAL-27 cells"],"prefix":"10.3389","volume":"11","author":[{"given":"Kaiqi","family":"Tang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han","family":"Su","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi","family":"Qu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,8,3]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"e07370","DOI":"10.1016\/j.heliyon.2021.e07370","article-title":"Novel greenly synthesized titanium dioxide nanoparticles compared to liposomes in drug delivery: In vivo investigation on ehrlich solid tumor model","volume":"7","author":"Abdel Fadeel","year":"2021","journal-title":"Heliyon"},{"key":"B2","doi-asserted-by":"publisher","first-page":"249","DOI":"10.3390\/molecules25020249","article-title":"UV\/Vis light induced degradation of oxytetracycline hydrochloride mediated byCo-TiO2 nanoparticles","volume":"25","author":"Akel","year":"2020","journal-title":"Molecules"},{"key":"B3","doi-asserted-by":"publisher","first-page":"1359","DOI":"10.3390\/pharmaceutics14071359","article-title":"Ciprofloxacin-loaded titanium nanotubes coated with chitosan: A promising formulation with sustained release and enhanced antibacterial properties","volume":"14","author":"Asadi","year":"2022","journal-title":"Pharmaceutics"},{"key":"B4","doi-asserted-by":"publisher","first-page":"718356","DOI":"10.3389\/fnut.2021.718356","article-title":"Inflammatory and microbiota-related regulation of the intestinal epithelial barrier","volume":"8","author":"Barbara","year":"2021","journal-title":"Front. 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