{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T21:51:52Z","timestamp":1782942712841,"version":"3.54.5"},"reference-count":56,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T00:00:00Z","timestamp":1697068800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Bioeng. Biotechnol."],"abstract":"<jats:p><jats:bold>Introduction:<\/jats:bold> Mechanical loading is known to determine the course of bone fracture healing. We hypothesise that lower limb long bone loading differs with knee flexion angle during walking and frontal knee alignment, which affects fracture healing success.<\/jats:p><jats:p><jats:bold>Materials and methods:<\/jats:bold> Using our musculoskeletal <jats:italic>in silico<\/jats:italic> modelling constrained against <jats:italic>in vivo<\/jats:italic> data from patients with instrumented knee implants allowed us to assess internal loads in femur and tibia. These internal forces were associated with the clinical outcome of fracture healing in a relevant cohort of 178 extra-articular femur and tibia fractures in patients using a retrospective approach.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> Mean peak forces differed with femoral compression (1,330\u20131,936\u00a0N at mid-shaft) amounting to about half of tibial compression (2,299\u20135,224\u00a0N). Mean peak bending moments in the frontal plane were greater in the femur (71\u2013130\u00a0Nm) than in the tibia (from 26 to 43\u00a0Nm), each increasing proximally. Bending in the sagittal plane showed smaller mean peak bending moments in the femur (\u221238 to 43\u00a0Nm) reaching substantially higher values in the tibia (\u221263 to \u2212175\u00a0Nm) with a peak proximally. Peak torsional moments had opposite directions for the femur (\u221213 to \u221240\u00a0Nm) versus tibia (15\u201348\u00a0Nm) with an increase towards the proximal end in both. Femoral fractures showed significantly lower scores in the modified Radiological Union Scale for Tibia (mRUST) at last follow-up (<jats:italic>p<\/jats:italic> &amp;lt; 0.001) compared to tibial fractures. Specifically, compression (<jats:italic>r<\/jats:italic> = 0.304), sagittal bending (<jats:italic>r<\/jats:italic> = 0.259), and frontal bending (<jats:italic>r<\/jats:italic> = \u22120.318) showed strong associations (<jats:italic>p<\/jats:italic> &amp;lt; 0.001) to mRUST at last follow-up. This was not the case for age, body weight, or localisation alone.<\/jats:p><jats:p><jats:bold>Discussion:<\/jats:bold> This study showed that moments in femur and tibia tend to decrease towards their distal ends. Tibial load components were influenced by knee flexion angle, especially at push-off, while static frontal alignment played a smaller role. Our results indicate that femur and tibia are loaded differently and thus require adapted fracture fixation considering load components rather than just overall load level.<\/jats:p>","DOI":"10.3389\/fbioe.2023.1284091","type":"journal-article","created":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T15:31:16Z","timestamp":1697124676000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":12,"title":["Lower-limb internal loading and potential consequences for fracture healing"],"prefix":"10.3389","volume":"11","author":[{"given":"Mark","family":"Heyland","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominik","family":"Deppe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie Jacqueline","family":"Reisener","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philipp","family":"Damm","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William R.","family":"Taylor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon","family":"Reinke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georg N.","family":"Duda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam","family":"Trepczynski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,10,12]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"911058","DOI":"10.3389\/fendo.2022.911058","article-title":"Validation of the modified radiographic union score for tibia fractures (mRUST) in murine femoral fractures","volume":"13","author":"Alentado","year":"2022","journal-title":"Front. 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